Browsing by Author "Tsai, May-Jywan"

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Accurate real-time sensing tip for aqueous NO with optical fibers embedded in active hydrogel waveguide
(2018-02-01)
Chung, Chieh-Wen
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Tsai, May-Jywan
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Lin, Peng-Wei
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Huang, Ding-Wen
;
Wang, Kuan-Hsun
A NO sensing tip is made by inserting two parallel optical fibers inside a poly 2-hydroxyethyl methacrylate (PolyHEMA) hydrogel waveguide mixed with the probe molecule 1, 2-Diaminoanthraquinone (DAQ). There is a length difference of 1 mm between the two fibers, and the light has to propagate through the difference from the short fiber to the long fiber. The total cross section area of the active hydrogel waveguide embedded with the fibers is only 3mm x 1.2 mm. For practical use the tip is housed in a needle for mechanical protection and the sensing tip is able to detect aqueous NO concentration around 1 mu M with time resolution about 5 minutes. Such a sensing tip can be used to monitor the medical conditions inside the brain after a stroke or a brain injury. (C) 2018 Author(s).
Conversion of absorption to fluorescence probe in solid-state sensor for nitric oxide and nitrite
(2013-04-01)
Hong, Wun-Yan
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Yang, Tsung-Wei
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Wang, Chun-Ming
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Syu, Jia-Hao
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Lin, Yu-Chao
A hydrogel bi-layer structure is used to convert an absorption type to a more convenient fluorescent type probe film for aqueous nitric oxide (NO) and nitrite (NO2-). In the bottom layer the commercial probe molecule 1,2-diaminoanthraquinone (DAQ) is dispersed in poly 2-hydroxyethyl methacrylate (poly HEMA), in the top layer the red inorganic phosphor is dispersed in poly HEMA. Both optical excitation and detection is from the bottom. This bi-layer is integrated with organic light-emitting diode (OLED) and organic photo-detector to form a real-time solid-state sensor. The sensor is responsive to NO bubbling, and remains stable in acid condition. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
Detecting Breath Ammonia for Non-invasive Diagnostic based on Low-Cost Organic Diode with Vertical Nanojunctions
(2012)
Dai, Ming-Zhi
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Lin, Yi-Lo
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Lin, Hong-Cheng
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Zan, Hsiao-Wen
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Chang, Kai-Ting
We propose a novel solid-state electrical ammonia sensor based on a vertical organic diode with high-density nanopores in the top electrode. The proposed ammonia sensor has a detection limit of 20 ppb, a real-time response, sufficient selectivity, simple structure, high reproducibility, and a low production cost. In addition, we used the proposed sensor to detect the nasal breath of rats directly. After controlling the relative humidity and filtering out the CO2, we successfully obtained repeated real-time, reliable responses. This is the first demonstration of a solid-state sensor having reliable responses to the breath ammonia of an animal. The sensing response between normal and acute groups is significantly different (p < 0.05). These results demonstrated that the proposed ammonia sensor can detect liver that undergoes moderate hepatopathy.
Electrospun Fibers as a Solid-State Real-Time Zinc Ion Sensor with High Sensitivity and Cell Medium Compatibility
(2013-03-25)
Syu, Jia-Hao
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Cheng, Yi-Kai
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Hong, Wun-Yan
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Wang, Hsing-Ping
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Lin, Yu-Chao
meso-2,6-Dichlorophenyltripyrrinone (TPN-Cl2), a probe molecule for zinc II ions, is dispersed in a polymer host. The red fluorescence peak at 620 nm appears when the molecule forms a complex with zinc at its center. TPN-Cl2 has a high selectivity for zinc II and tolerates many common metal ions present in the human body. The probe molecules are blended with a hydrogel polymer, poly(2-hydroxyethyl methacrylate) (poly HEMA), with 30 wt% dimethylformamide (DMF). The fiber structure with 1 m diameter is made by electrospinning in DMF solution of the probe and poly HEMA mixture. The fibrous film detects zinc ions with concentrations as low as 106 M in real-time both in water and in the commonly used cell culture liquid media Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS), which contain many metal ions and proteins. The time-resolution is 5 min for 106 M and 1 min for 105 M. This sensitivity and response speed satisfy the requirements for non-invasive biomedical studies.
Highly Sensitive Ammonia Sensor with Organic Vertical Nanojunctions for Noninvasive Detection of Hepatic Injury
(2013-03-19)
Dai, Ming-Zhi
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Lin, Yi-Lo
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Lin, Hung-Cheng
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Zan, Hsiao-Wen
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Chang, Kai-Ting
We successfully demonstrate the first solid-state sensor to have reliable responses to breath ammonia of rat. For thioacetamide (TAA)-induced hepatopathy rats, we observe that the proposed sensor can detect liver that undergoes acute-moderate hepatopathy with a p-value less than 0.05. The proposed sensor is an organic diode with vertical nanojunctions produced by using low-cost colloidal lithography. Its simple structure and low production cost facilitates the development of point-of-care technology. We also anticipate that the study is a starting point for investigating sophisticated breath-ammonia-related disease models.
Hydrogel-based zinc ion sensor on optical fiber with high resolution and application to neural cells
(2020-08-15)
Tsai, May-Jywan
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Cheng, Henrich
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Ho, Hsin-Hsien
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Lin, Peng-Wei
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Liou, Dann-Ying
Solid-state zinc ion sensor is developed with high enough resolution and reproducibility for the potential application in brain injury monitoring. An optical diffuser is incorporated into the zinc ion sensor based on optical fiber and hydrogel doped with the fluorescent zinc ion probe molecule meso-2,6-Dichlomphenyltripyrrinone (TPN-Cl-2). The diffuser transforms the high-peak-intensity excitation light near the fiber end into a broad light with moderate local intensity to reduce the degradation of the probe molecule. Reversible detection can be reached for 1, 2, and 5 mu m (10(-6) Molar), with slopes 0.3, 0.6, and 0.8 respectively. This is the pathophysiological concentration range after brain injury. The sensor is applied to neuron-glial cultures and macrophage under the stimulation of lipopolysaccharide (LPS), KCl and oxygen/glucose deprivation (OGD) that reflect inflammation, depolarization and ischemia respectively, mimicking events after brain injury. The zinc ion level is raised to 4-5 mu m after LPS treatment, and then reduced to <3 mu m after the co-treatment with the herbal drug silymarin. The results suggest the conditions of the neural cells under stress can be monitored.
Solid-state sensing tip for zinc ion with double parallel optical fibers embedded in fluorescent hydrogel
(2015-11-01)
Su, Heng-Tsan
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Tsai, May-Jywan
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Chang, Gao-Fong
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Hung, Chen-Hsiung
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Lin, Hung-Cheng
A tip-shaped zinc ion solid-state sensor is made by two parallel optical fibers embedded closely in a sensing hydrogel film. The film is made of poly(2-hydroxyethyl methacrylate) (poly HEMA) hydrogel mixed with the selective fluorescent probe meso-2,6-Dichlorophenyltripyrrinone (TPN-Cl-2) with weight ratio of 0.025 wt%. A 405 nm laser output is sent from one fiber and the 622 nm fluorescence of the doped hydrogel is collected by the second fiber. Each fiber diameter is 370 mu m (core is 300 mu m), whose sum is roughly the tip diameter. The 0.4 cm by 0.5 cm tip has real-time response for zinc ion concentration over 10(-6) M, with marginal signal for 10(-7) M. The tip is inserted inside an oyster and successfully detects the zinc ions, showing that the sensor works in complex body fluid and tolerates certain mechanical stress. To show the potential application for medicine, the sensing film is applied for primary neuronal cultures. We report for the first time zinc ions release at concentration levels 10(-6)-10(-7) M to the medium under stress conditions of ischemia, inflammation, and intoxication. Furthermore, this correlates with the zinc levels detected by biochemical assay. Such sensing tip has great potential for biomedical monitoring ex vivo or in vivo. (C) 2015 Elsevier B.V. All rights reserved.
Solid-state sensing tip for zinc ion with double parallel optical fibers embedded in fluorescent hydrogel (vol 26, pg 429, 2015)
(2016-08)
Su, Heng-Tsan
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Tsai, May-Jywan
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Chang, Gao-Fong
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Hung, Chen-Hsiung
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Lin, Hung-Cheng