Browsing by Author "Tsai, Meng-Zhe"

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Integration of a guided-mode resonance filter with microposts for in-cell protein detection
(2016)
Tu, Yi-Kai
;
Tsai, Meng-Zhe
;
Lee, I-Chin
;
Hsu, Hsin-Yun
;
Huang, Cheng-Sheng
We present an integrated microfluidic system consisting of a label-free biosensor of a guided-mode resonance filter (GMRF) and a microfluidic channel with a micropost filter. The GMRF was fabricated through replica molding using an ultraviolet-curable polymer and a plastic substrate. An array of microposts (a diameter and height of 26.5 and 56 mu m, respectively, and a spacing between 7.5 and 9.5 mu m), fabricated on a silicon substrate through photolithography, was used as the filter. A double-sided tape was used to laminate the GMRF and a microfluidic chip such that the integrated device provides two functions: filtration of the cell debris and quantification of the in-cell protein concentration. By measuring the changes in the resonant wavelength from the GMRF, the detection of beta-actin in an unprocessed lysed cell sample was demonstrated; the cell debris was separated using the micropost filter to prevent false measurement.
Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor
(2018-01-21)
Tsai, Meng-Zhe
;
Hsiung, Chan-Te
;
Chen, Yang
;
Huang, Cheng-Sheng
;
Hsu, Hsin-Yun
We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU-1, which supports a limit of detection of 3.2 ng mL(-1) for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.