Browsing by Author "Vedamalai, Mani"

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A BODIPY-based colorimetric and fluorometric chemosensor for Hg(II) ions and its application to living cell imaging
(2012)
Vedamalai, Mani
;
Wu, Shu-Pao
;
應用化學系
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Department of Applied Chemistry
A new monostyryl boron dipyrromethene derivative (MS1) appended with two triazole units indicates the presence of Hg2+ among other metal ions with high selectivity by color change and red emission. Upon Hg2+ binding, the absorption band of MS1 is blue-shifted by 29 nm due to the inhibition of the intramolecular charge transfer from the nitrogen to the BODIPY, resulting in a color change from blue to purple. Significant fluorescence enhancement is observed with MS1 in the presence of Hg2+; the metal ions Ag+, Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Fe3+, K+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+ cause only minor changes in the fluorescence of the system. The apparent association constant (K-a) of Hg2+ binding in MS1 is found to be 1.864 x 10(5) M-1. In addition, fluorescence microscopy experiments show that MS1 can be used as a fluorescent probe for detecting Hg2+ in living cells.
A BODIPY-Based Highly Selective Fluorescent Chemosensor for Hg2+ Ions and Its Application in Living Cell Imaging
(2012-02-01)
Vedamalai, Mani
;
Wu, Shu-Pao
;
應用化學系
;
Department of Applied Chemistry
A new borondipyrromethene (BODIPY) derivative (FS1) containing two triazole units exhibits an enhanced fluorescence in the presence of Hg2+ ions and a high selectivity for Hg2+ ions over competing metal ions in methanol: Ag+, Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Fe3+, K+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+ produced only minor changes in the fluorescence of FS1. The apparent dissociation constant (Kd) of FS1Hg2+ was found to be 62 mu M. Moreover, fluorescence microscopy experiments showed that FS1 can be used as a fluorescent probe for detecting Hg2+ ions in living cells.
Hypochlorous acid turn-on boron dipyrromethene probe based on oxidation of methyl phenyl sulfide
(2013-10-24)
Liu, Shi-Rong
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Vedamalai, Mani
;
Wu, Shu-Pao
;
應用化學系
;
Department of Applied Chemistry
A boron dipyrromethene (BODIPY)-based fluorometric probe, HCS, has been successfully developed for the highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of methyl phenyl sulfide. The reaction is accompanied by a 160-fold increase in the fluorescent quantum yield (from 0.003 to 0.480). The fluorescent turn-on mechanism is accomplished by suppression of photoinduced electron transfer (PET) from the methyl phenyl sulfide group to BODIPY. The fluorescence intensity of the reaction between HOCl and HCS shows a good linearity in the HOCl concentration range 1-10 mu M. The detection limit is 23.7 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HCS probe could be an efficient fluorescent detector for HOCl in living cells. (C) 2013 Published by Elsevier B.V.