Browsing by Author "Wang, Chien-Chia"

Now showing 1 - 3 of 3

Divergent Alanyl-tRNA Synthetase Genes of Vanderwaltozyma polyspora Descended from a Common Ancestor through Whole-Genome Duplication Followed by Asymmetric Evolution
(2015-07-01)
Chang, Chia-Pei
;
Chang, Chih-Yao
;
Lee, Yi-Hsueh
;
Lin, Yeong-Shin
;
Wang, Chien-Chia
Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. However, in most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. Here, we present a novel scenario of AlaRS evolution in the yeast Vanderwaltozyma polyspora. Vanderwaltozyma polyspora possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Clever selection of transcription and translation initiation sites enables the two isoforms to be localized and thus functional in their respective cellular compartments. However, the two isoforms can also be stably expressed and function in the reciprocal compartments by insertion or removal of a mitochondrial targeting signal. Synteny and phylogeny analyses revealed that the AlaRS homologues of Vanderwaltozyma polyspora arose from a dual-functional common ancestor through whole-genome duplication (WGD). Moreover, the mitochondrial form had higher synonymous (1.6-fold) and nonsynonymous (2.8-fold) substitution rates than did its cytoplasmic counterpart, presumably due to a lesser constraint imposed on components of the mitochondrial translational apparatus. Our study suggests that asymmetric evolution confers the divergence between the AlaRS paralogues of Vanderwaltozyma polyspora.
Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase
(2017-07-01)
Lee, Yi-Hsueh
;
Chang, Chia-Pei
;
Cheng, Yu-Ju
;
Kuo, Yi-Yi
;
Lin, Yeong-Shin
The discriminator base N73 is a key identity element of tRNA(His). In eukaryotes, N73 is an "A" in cytoplasmic tRNA(His) and a "C" in mitochondrial tRNA(His). We present evidence herein that yeast histidyl-tRNA synthetase (HisRS) recognizes both A73 and C73, but somewhat prefers A73 even within the context of mitochondrial tRNA(His). In contrast, humans possess two distinct yet closely related HisRS homologues, with one encoding the cytoplasmic form (with an extra N-terminal WHEP domain) and the other encoding its mitochondrial counterpart (with an extra N-terminal mitochondrial targeting signal). Despite these two isoforms sharing high sequence similarities (81% identity), they strongly preferred different discriminator bases (A73 or C73). Moreover, only the mitochondrial form recognized the anticodon as a strong identity element. Most intriguingly, swapping the discriminator base between the cytoplasmic and mitochondrial tRNA(His) isoacceptors conveniently switched their enzyme preferences. Similarly, swapping seven residues in the active site between the two isoforms readily switched their N73 preferences. This study suggests that the human HisRS genes, while descending from a common ancestor with dual function for both types of tRNA(His), have acquired highly specialized tRNA recognition properties through evolution.
Low Cost Facile Synthesis of Large-Area Cobalt Hydroxide Nanorods with Remarkable Pseudocapacitance
(2015-05-06)
Deng, Ming-Jay
;
Song, Cheng-Zhao
;
Wang, Chien-Chia
;
Tseng, Yuan-Chieh
;
Chen, Jin-Ming
Large-area Co(OH)(2)-based supercapacitor electrodes composed of nanotube arrays grown on a 3D nickel-foam (CONTA) electrode and sucker-like nanoporous films grown on a 3D nickel-foam (COSNP) electrode were prepared with a facile electrochemical method for applications in energy storage. These nanoporous Co(OH)(2) electrodes were fabricated with the codeposition of Cu/Ni film on the nickel foam, then etching of Cu from the Cu/Ni layer to form Ni nanotube arrays and sucker-like Ni nanoporous layers, and further cathodic deposition of Co(OH)(2) on the prepared nanoporous Ni substrates. The CONTA and COSNP electrodes exhibited specific capacitances of 2500 and 2900 F/g in a voltage range of 0.65 V (capacitance of the substrates deducted from the total) at 1 A/g in a three electrode cell, respectively. The COSNP electrode demonstrated an excellent supercapacitive performance with specific capacitances 1100 F/g at 1 A/g and 850 F/g at 20 A/g in a voltage range of 1.2 V in a two electrode cell. The remarkable performance of COSNP electrodes correlated with a large conversion of the Co oxidation state during the charge/discharge cycling were examined by in situ X-ray absorption near edge structure (XANES).