Browsing by Author "Wang, Yu-Liang"

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A water soluble and fast response fluorescent turn-on copper complex probe for H2S detection in zebra fish
(2016-01-15)
Palanisamy, Sathyadevi
;
Lee, Lu-Ying
;
Wang, Yu-Liang
;
Chen, Yu-Jen
;
Chen, Chiao-Yun
According to the displacement method, herein we reported a water soluble copper complex [Cu(MaT-cyclen)(2)] as a fluorescent probe for the detection of H2S. For this, 1((1-(10-methylanthracen-9-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-1,4,7,10-tetraazacyclododecane (MaT-cyclen) was synthesized first To improve its solubility in aqueous media, sodium acetate group was introduced into 8-hydroxy-2-quinoline successfully. MaT-cyclen was chelated with Cu(II) to form [Cu(MaT-cyclen)2] complex, which displayed high sensitivity and selectivity for H2S over the other possible competitive substances on the basis of forming CuS. Meanwhile, [Cu(MaT-cyclen)(2)] displayed rapid response (< 1 min), well reversibility, lowest detection limit (205 nM), and high sensitivity for recognizing H2S in aqueous solution. Furthermore, its potential utility for biological applications was confirmed by fluorescence imaging of H2S in live cells as well as in zebra fish. (C) 2015 Elsevier B.V. All rights reserved.
In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish
(2018-10-01)
Palanisamy, Sathyadevi
;
Wang, Yu-Liang
;
Chen, Yu-Jen
;
Chen, Chiao-Yun
;
Tsai, Fu-Te
Nitroxyl (HNO) plays a critical role in many physiological processes which includes vasorelaxation in heart failure, neuroregulation, and myocardial contractility. Powerful imaging tools are required to obtain information for understanding the mechanisms involved in these in vivo processes. In order to develop a rapid and high sensitive probe for HNO detection in living cells and the zebrafish model organism, 2-((2-(benzothiazole-2yl)benzylidene) amino)benzoic acid (AbTCA) as a ligand, and its corresponding copper(II) complex Cu(II)-AbTCA were synthesized. The reaction results of Cu(II)-AbTCA with Angeli's salt showed that Cu(II)-AbTCA could detect HNO quantitatively in a range of 40-360 mu M with a detection limit of 9.05 mu M. Furthermore, Cu(II)-AbTCA is more selective towards HNO over other biological species including thiols, reactive nitrogen, and reactive oxygen species. Importantly, Cu(II)-AbTCA was successfully applied to detect HNO in living cells and zebrafish. The collective data reveals that Cu(II)-AbTCA could be used as a potential probe for HNO detection in living systems.