Browsing by Author "Wu, HT"

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A CMOS 33-mW 100-MHz 80-dB SFDR sample-and-hold amplifier
(2003-01-01)
Hsu, CC
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Wu, HT
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電子工程學系及電子研究所
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Department of Electronics Engineering and Institute of Electronics
A high-speed high-resolution sample-and-hold amplifier (SHA) is designed for time-interleaved analog-to-digital converter applications. Using the techniques of precharging and output capacitor coupling can mitigate the stringent performance requirements for the opamp, resulting in low power dissipation. Implemented in a standard 0.25 mum CMOS technology, the SHA achieves 80 dB spurious-free dynamic range (SFDR) for a 1.8 Vpp output at 100 MHz Nyquist sampling rate. The SHA occupies a die area of 0.35 mm(2) and dissipates 33 mW from a single 2.5 V supply.
Effects of glucose concentration on in vitro fertilization in BALB/c mice
(2003-12-01)
Wu, HT
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Chou, CK
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Lin, CS
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Huang, MC
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生物科技學系
BALB/c mice are widely used in genetic, tumour and immunological studies. However, the mice demonstrate a lower reproduction rate, low fertility and small litters, because of their highly genetic homozygoisty. Based on in vitro fertilization (IVF), a routine technique for biomedical studies, it is worth to evaluate the effects to BALB/c mice on IVF efficiency. In order to test the genetic factor affecting the IVF efficiency of BALB/c, four reciprocal IVF tests of BALB/cByJ and FVB/NCrl mice were performed. The results showed that the average fertility of IVF sponsored by FVB/NCrl spermatozoa was 69.6%, but only 12.1% was obtained from BALB/cByJ strain. Effect of glucose contained in the culture medium to the IVF efficiency of BALB/cByJ was also evaluated. The results showed that the fertility of BALB/cByJ spermatozoa incubated with 0, 2.7, 5.5, 11.1 and 22.2 mM of glucose in the TYH medium were 6.8, 9.9, 13.9, 32.7 and 22.2%, respectively. It is showed that IVF efficiency of BALB/cByJ spermatozoa could be improved depending on the concentration of glucose in the IVF medium. According to the results, it is beleived that lower IVF of BALB/cByJ mice might be due to the genetic defect in spermatozoa and increasing glucose in the IVF medium which significantly affect the IVF efficiency of BALB/cByl via activating the spermatozoa.
In vitro and ex vivo green fluorescent protein expression in alveolar mammary epithelial cells and mammary glands driven by the distal 5 '-regulative sequence and intron 1 of the goat beta-casein gene
(2003)
Wu, HT
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Lin, CS
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Huang, MC
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生物科技學系
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Department of Biological Science and Technology
The 5'-regulative sequence and intron 1 of the goat beta-casein gene from -4044 to +2123 by was cloned and fused with the reporter gene of green fluorescent protein (GFP) to create a plasmid termed pGB562/GFP To detect GFP expression, pGB562/GFP was transfected in vitro via liposomes into the mammary epithelial cell line NMuMG. Cells could not express GFP unless the transfected NMuMG cells lined up to create functional alveoli. These functional cells were cultured with lactogenic hormones, including insulin, dexamethasone and prolactin, and were grown on a layer of the extracellular matrix Matrigel. Green fluorescent protein expression levels in NMuMG cells were 25-, 55- and 42-fold those in the control group at 24, 48, and 72 h after pGB562/GFP transfection respectively. In addition, pGB562/GFP was transfected ex vivo by electroporation into mammary gland fragments and cells were then cultured in vitro with a supplement of lactogenic hormones. Strong GFP expression localized in fragments of the mammary gland was observed 24 h after gene transfer. The novel strategy of ex vivo gene transfer into mammary tissue using GFP as a reporter gene to detect the function of a tissue-specific promoter is efficient and convenient. The data obtained herein reveal that the 5'-regulative sequence and intron 1 of the 6.2 kb goat beta-casein gene can enhance the efficiency of transgene expression. Thus, the GB562 sequence may act as a good promoter and effectively elevate the production of exogenous protein in mammary glands.
Ping-pong flow control for ATM ABR traffic
(2003-09-01)
Ku, CY
;
Hwang, LC
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Wu, HT
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電信工程研究所
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Institute of Communications Engineering
In this paper, we propose an improved technique for congestion control, named as ping-pong flow control (PPFC), for asynchronous transfer mode (ATM) available bit rate (ABR) traffic. This is a rate-based flow control scheme, in which the rate regulation is achieved by directly adjusting the transmission rate in the source end station. The proposed algorithm uses a bipolar feedback strategy, which employs positive and negative feedbacks to control the transmission rate for different switch states. These states are determined using the traditional threshold-based method. We also introduce state early detection (SED), which enables the PPFC to control traffic flows more precisely and accurately at critical moments. The simulation results show that the proposed algorithm provides a higher throughput and lower cell loss ratio when compared to the well-known backward explicit congestion notification (BECN). Furthermore, these results also show that PPFC is robust against feedback losses. (C) 2003 Elsevier Science B.V. All rights reserved.