Browsing by Author "Wu, Kun-Yu"

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Envelope Structure of Human eNOS Protein Revealed by Small-Angle X-ray Scattering
(2012-04-01)
Chen, Chun-Yu
;
Chen, Pei-Feng
;
Hwu, Yeukuang
;
Jeng, U-Ser
;
Wu, Kun-Yu
We investigate the solution structure of human eNOS protein by using synchrotron small-angle X-ray scattering (SAXS). The pair-correlation analysis of the profile shows the radius of gyration (R-g) and maxima dimension (D-max) of 6.87 +/- 0.03 nm and 22 nm, respectively. The ratio of D-max and R-g revealed that the protein was an extended conformation. The ab initio shape determination and rigid-body calculations were performed to reconstruct the real-space structure of eNOS in solution. The result shows that human eNOS form homodimer form in solution with the closed contact of two oxygenase domains.