Browsing by Author "Wu, Ming-Chun"

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A turn-on fluorescent sensor for cysteine based on BODIPY functionalized Au nanoparticles and its application in living cell imaging
(2015-12-31)
Lo, Shu-Hua
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Wu, Ming-Chun
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Wu, Shu-Pao
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應用化學系
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Department of Applied Chemistry
BODIPY functionalized Au nanoparticles (PyBDP-AuNPs) have been developed for detecting cysteine. Gold nanoparticles were prepared by reducing HAuCl4 with sodium citrate, using PyBDP as the capping agent. Upon addition of cysteine, PyBDP molecules are liberated from the AuNP surface through ligand-exchange displacement based on the strong affinity of Au with the thiol group and result in green fluorescence from PyBDP molecules. This cysteine-induced displacement of PyBDP-AuNPs was monitored by fluorescent spectroscopy with a detection limit of 1.2 mu M. Optimal detection of cysteine was achieved over a pH range from 5 to 12. In addition, PyBDP-AuNPs can be used as a fluorescent probe for detecting cysteine in living cells. (C) 2015 Elsevier B.V. All rights reserved.
Colorimetric detection of chromium(III) using O-phospho-L-serine dithiocarbamic acid functionalized gold nanoparticles
(2015-12-01)
Lo, Shu-Hua
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Wu, Ming-Chun
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Venkatesan, Parthiban
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Wu, Shu-Pao
;
應用化學系
A sensitive and selective colorimetric Cr3+ detection method was developed by using O-phospho-L-serine dithiocarbamic acid (PSDTC) functionalized gold nanoparticles (PSDTC-AuNPs). The presence of Cr3+ immediately induced aggregation of PSDTC-AuNPs, yielding a color change from red to blue. This Cr3+-induced aggregation of PSDTC-AuNPs was monitored by bare eye and UV-vis spectroscopy. The SPR absorbance (651 nm) of the reaction between PSDTC-AuNPs and Cr3+ shows a good linearity in the Cr3+ concentration with a low detection limit 4.2 nM (S/N = 3). PSDTC-AuNPs showed excellent selectivity toward Cr3+ compared to other metal ions Ag+, Al3+, Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Fe3+, Hg2+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+. Optimal detection of Cr3+ was achieved over a pH range from 5 to 10. In addition, PSDTC-AuNPs were applied to detect Cr3+ in lake water, showing low interference. (C) 2015 Elsevier B.V. All rights reserved.