Browsing by Author "Wu, Ming-Fang"

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Integrated multiple multi-photon imaging and Raman spectroscopy for characterizing structure-constituent correlation of tissues
(2008-10-13)
Jhan, Jhen-Wei
;
Chang, Wei-Tien
;
Chen, Hung-Che
;
Lee, Yu-Tsung
;
Wu, Ming-Fang
Histopathological imaging of tissues often requires extensive sample preparation including fixation and staining in order to highlight characteristic alterations associated with diseases. Herein, we report an integrated spectro-microscopy approach based on a combination of multimodal multi-photon imaging and Raman micro-spectroscopy and demonstrate label-free characterization of the structure-constituent correlation of porcine skin. The multi-modal imaging allows the visualization of dermatological features whereas Raman micro-spectroscopy enables the identification of their 'molecular fingerprints'. By obtaining both structural and molecular-level information of tissue constituents, this integrated approach can offer new insight into the patho-physiological status of tissues. (c) 2008 Optical Society of America