Browsing by Author "Yang, Jyh-Yuan"

Now showing 1 - 4 of 4

Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy
(2013-07-19)
Li, Hsing-Yuan
;
Tseng, Shing-Hua
;
Cheng, Tsai-Mu
;
Chu, Hsueh-Liang
;
Lu, Yu-Ning
Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 mu 1 reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.
Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy (vol 24, 285102, 2013)
(2013-10-04)
Li, Hsing-Yuan
;
Tseng, Shing-Hua
;
Cheng, Tsai-Mu
;
Chu, Hsueh-Liang
;
Lu, Yu-Ning
Rapid Detection and Quantification of Enterovirus 71 by Electrochemical Impedance Spectroscopy
(2012-01-31)
Li, Hsing-Yuan
;
Yang, Jyh-Yuan
;
Cheng, Tsia-Mu
;
Tseng, Shin-Hua
;
Chang, Chia-Ching
ULTRA SENSITIVE DETECTION OF ENETEROVIRUS 71 BY MODIFIED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY
(2010)
Tseng, Shin-Hua
;
Tseng, Dion T.
;
Lee, Tzu-Cheng
;
Cheng, Tsai-Mu
;
Yang, Jyh-Yuan
The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (similar to sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.