Browsing by Author "Yang, Yi-Wei"

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A Cold Planar Nitrogen-Based Atmospheric-Pressure Dielectric Barrier Discharge Jet With Enhanced UV Emission and Radical Generation Using Short Electrodes
(2016-12)
Cheng, Kuang-Yao
;
Yang, Yi-Wei
;
Lin, Zhi-Hua
;
Liao, Guo-Chun
;
Liu, Chih-Tung
In this paper, a planar nitrogen-based atmospheric-pressure dielectric barrier discharge (DBD) jet with enhanced UV emission and radical generation, and much lower power consumption and gas temperature using shorter electrodes as compared with a previously developed DBD jet is presented. The highest UV intensity (five times larger) and radical generation (two times larger) in the DBD jet region are found to occur when the electrode length is 1 cm. Corresponding jet gas temperature and power consumption are 31 degrees C and 39 W, respectively. The 1-cm DBD jet was used to treat polylactide film for enhancing its hydrophilic properties. The results show that a contact angle of 28.4 degrees could be obtained with a residence time of 4 s. The resulting 1-cm DBD jet may provide an efficient surface treatment tool for delicate industrial and biomedical devices/materials.
A Cold Planar Nitrogen-Based Atmospheric-Pressure Dielectric Barrier Discharge Jet With Enhanced UV Emission and Radical Generation Using Short Electrodes
(2016-12-01)
Cheng, Kuang-Yao
;
Yang, Yi-Wei
;
Lin, Zhi-Hua
;
Liao, Guo-Chun
;
Liu, Chih-Tung
In this paper, a planar nitrogen-based atmospheric-pressure dielectric barrier discharge (DBD) jet with enhanced UV emission and radical generation, and much lower power consumption and gas temperature using shorter electrodes as compared with a previously developed DBD jet is presented. The highest UV intensity (five times larger) and radical generation (two times larger) in the DBD jet region are found to occur when the electrode length is 1 cm. Corresponding jet gas temperature and power consumption are 31 degrees C and 39 W, respectively. The 1-cm DBD jet was used to treat polylactide film for enhancing its hydrophilic properties. The results show that a contact angle of 28.4 degrees could be obtained with a residence time of 4 s. The resulting 1-cm DBD jet may provide an efficient surface treatment tool for delicate industrial and biomedical devices/materials.
Aerosol-Assisted Plasma Deposition of Biocomposite Coatings: Investigation of Processing Conditions on Coating Properties
(2016-12)
Hsiao, Chun-Ping
;
Wu, Cheng-Chen
;
Liu, Yung-Hsin
;
Yang, Yi-Wei
;
Cheng, Yun-Chien
The aerosol-assisted atmospheric-pressure planar dielectric-barrier-discharge-type plasma deposition (AA-APPD) system can polymerize monomers and immobilize biomolecules simultaneously. In this paper, we used this technology to deposit biocomposite thin films of polyethylene (PE) along with lysozyme (Lyz) under different aerosol solution temperatures and at different coating positions in the discharge region. In addition, we compared the deposition efficacy of two kinds of Lyz solution using deionized (DI)-water and phosphate-buffered saline (PBS) as solvents, respectively. The results show that deposition rate of thin films increases with increasing solution temperature at most coating positions. In addition, coatings from Lyz-PBS perform higher deposition rates, but with weaker Lyz viability as compared with that of Lyz DI-water. Finally, we also verified that this DBD-type AA-APPD system can not only successfully deposit Lyz in PE thin film, but also maintain Lyz viability after plasma process under different solution temperatures and positions.
Aerosol-Assisted Plasma Deposition of Biocomposite Coatings: Investigation of Processing Conditions on Coating Properties
(2016-12-01)
Hsiao, Chun-Ping
;
Wu, Cheng-Chen
;
Liu, Yung-Hsin
;
Yang, Yi-Wei
;
Cheng, Yun-Chien
The aerosol-assisted atmospheric-pressure planar dielectric-barrier-discharge-type plasma deposition (AA-APPD) system can polymerize monomers and immobilize biomolecules simultaneously. In this paper, we used this technology to deposit biocomposite thin films of polyethylene (PE) along with lysozyme (Lyz) under different aerosol solution temperatures and at different coating positions in the discharge region. In addition, we compared the deposition efficacy of two kinds of Lyz solution using deionized (DI)-water and phosphate-buffered saline (PBS) as solvents, respectively. The results show that deposition rate of thin films increases with increasing solution temperature at most coating positions. In addition, coatings from Lyz-PBS perform higher deposition rates, but with weaker Lyz viability as compared with that of Lyz DI-water. Finally, we also verified that this DBD-type AA-APPD system can not only successfully deposit Lyz in PE thin film, but also maintain Lyz viability after plasma process under different solution temperatures and positions.
An Optimized Two-Step Nitrogen-Based Plasma Treatment Procedure under Atmospheric-Pressure Condition and its Surface Amination Mechanism
(2015-07-01)
Yang, Yi-Wei
;
Wu, Jane-Yii
;
Liu, Chih-Tung
;
Liao, Guo-Chun
;
Cheng, Kuang-Yao
Atmospheric pressure nitrogen-based dielectric barrier discharge (DBD) jet with two-step plasma treatment procedure has been verified to be very effective in facilitating nitrogen-containing group incorporation on Polylactide (PLA) surface. It includes: 1) pretreatment plasma treatment by a nitrogen discharge with a trace amount of oxygen, and 2) ammonia plasma treatment by a nitrogen discharge with a small amount of ammonia. In this study, the optimized operation condition of two-step plasma procedure is investigated under different concentrations of added gases (0-1% oxygen; 0-10% ammonia). XPS analysis shows that approximate to 0.19 of N/C ratio can be achieved by means of the optimized two-step plasma procedures. Furthermore, a surface amination mechanism is proposed based on the observations of Optical Emission Spectra (OES), Atomic Force Microscope (AFM), and X-ray Photoelectron Spectra (XPS) analyses.
Atomic Oxygen and Hydroxyl Radical Generation in Round Helium-Based Atmospheric-Pressure Plasma Jets by Various Electrode Arrangements and Its Application in Sterilizing Streptococcus mutans
(2014-12-01)
Liu, Chih-Tung
;
Wu, Ching-Jung
;
Yang, Yi-Wei
;
Lin, Zhi-Hua
;
Wu, Jong-Shinn
The reactive chemical species generated in an atmospheric-pressure plasma jet (APPJ), such as hydroxyl radical and atomic oxygen, are generally regarded as the major sources for several biomedical applications. In this paper, we propose an APPJ with a 25-kHz high-voltage powered stainless steel tube fixed inside a quartz tube and an aluminum grounded electrode that is wrapped around the quartz tube. The results show that the radical intensities of atomic oxygen and hydroxyl radical, gas temperature, and power absorption increase with increasing distance of discharge gap distance under the same applied voltage. Increasing the width of grounded electrode increases gas temperature and causes higher power absorption but only slight change of radical generation. We have applied the APPJ with the conditions of highest radical generation (grounded electrode width = discharge gap distance = 15 mm) for effectively sterilizing Streptococcus mutans within 15 s, which shows a highly potential application for root canal disinfection.
Deposition of Hydroxyl Functionalized Films by Means of ethylene Aerosol-Assisted Atmospheric Pressure Plasma
(2014-11-01)
Yang, Yi-Wei
;
Camporeale, Giuseppe
;
Sardella, Eloisa
;
Dilecce, Giorgio
;
Wu, Jong-Shinn
The atmospheric pressure plasma deposition of hydroxyl functionalized hydrocarbon films is reported in this work, with a reactor fed with water aerosol and ethylene. The effects of power and feed flow rates onto film chemistry have been investigated. Coatings produced with this approach can find application in the biomedical field, among others, as platforms for cell adhesion and proliferation. Results show that operating at 4 kHz provides a much higher amount of hydroxyl group in the coating compared with samples obtained at 11 kHz. After water immersion, the stability of the films and their amount of hydroxyl groups remain high. A simplified deposition mechanism is proposed.
Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films
(2015-11-01)
Palumbo, Fabio
;
Camporeale, Giuseppe
;
Yang, Yi-Wei
;
Wu, Jong-Shinn
;
Sardella, Eloisa
Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 mg/cm(2) as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.
Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films
(2015-11)
Palumbo, Fabio
;
Camporeale, Giuseppe
;
Yang, Yi-Wei
;
Wu, Jong-Shinn
;
Sardella, Eloisa
Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 mg/cm(2) as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.
Enhancement of cell growth on honeycomb-structured polylactide surface using atmospheric-pressure plasma jet modification
(2017-02-01)
Cheng, Kuang-Yao
;
Chang, Chia-Hsing
;
Yang, Yi-Wei
;
Liao, Guo-Chun
;
Liu, Chih-Tung
In this paper, we compare the cell growth results of NIH-3T3 and Neuro-2A cells over 72 h on flat and honeycomb structured PLA films without and with a two-step atmospheric-pressure nitrogen-based plasma jet treatment. We developed a fabrication system used for forming of a uniform honeycomb structure on PLA surface, which can produce two different pore sizes, 3-4 mu m and 7-8 mu m, of honeycomb pattern. We applied a previously developed nitrogen-based atmospheric-pressure dielectric barrier discharge (DBD) jet system to treat the PLA film without and with honeycomb structure. NIH-3T3 and a much smaller Neuro-2A cells were cultivated on the films under various surface conditions. The results show that the two-step plasma treatment in combination with a honeycomb structure can enhance cell growth on PLA film, should the cell size be not too smaller than the pore size of honeycomb structure, e.g., NIH-3T3. Otherwise, cell growth would be better on flat PLA film, e.g., Neuro-2A. (C) 2016 Elsevier B.V. All rights reserved.
Enhancing adhesion and polymerization of lipase-plasma-polymerized-ethylene coatings deposited with planar dielectric-barrier-discharge-type aerosol-assisted atmospheric-pressure plasma system
(2018-04-01)
Cheng, Yun-Chien
;
Hsiao, Chun-Ping
;
Liu, Yung-Hsin
;
Yang, Chu-Hao
;
Chiang, Chun-Yi
To enhance the adhesion and polymerization of lipase-plasma-polymerized-ethylene (lipase-PPE) coatings deposited by aerosol-assisted atmospheric plasma deposition (AAAPPD), the effects of voltage, ceramic-dielectric thickness, and pre-coated PPE layer were investigated. In AAAPPD system, ethylene and lipase-phosphate-buffered-saline aerosol were used as precursors to polymerize ethylene and embed lipase simultaneously. The deposited lipase-PPE coatings were analyzed with FTIR, SEM, and surface profiler. The results show that increasing voltage from 3 to 4 kV and decreasing ceramic-dielectric thickness from 1 to 0.38 mm improved the coating adhesion and polymerization. Also, depositing a PPE layer with high energy prior to lipase-PPE coating enhanced adhesion. These improvements of the biomolecule-PPE coatings facilitate further bioapplications of this new biomolecule-embedding method.
Fast incorporation of primary amine group into polylactide surface for improving C2C12 cell proliferation using nitrogen-based atmospheric-pressure plasma jets
(2014-01-01)
Yang, Yi-Wei
;
Wu, Jane-Yii
;
Liu, Chih-Tung
;
Liao, Guo-Chun
;
Huang, Hsuan-Yu
In this article, we report the development of the fast incorporation of primary amine functional groups into a polylactide (PLA) surface using the post-discharge jet region of an atmospheric-pressure nitrogen-based dielectric barrier discharge (DBD). Plasma treatments were carried out in two sequential steps: (1) nitrogen with 0.1% oxygen addition, and (2) nitrogen with 5% ammonia addition. The analyses show that the concentration of N/C ratio, surface energy, contact angle, and surface roughness of the treated PLA surface can reach 19.1%, 70.5 mJ/m(2), 38 degrees and 73.22 nm, respectively. In addition, the proposed two-step plasma treatment procedure can produce a PLA surface exhibiting almost the same C2C12 cell attachment and proliferation performance as that of the conventional gelatin coating method. Most importantly, the processing/preparation time is reduced from 13-15 h (gelatin coating method) to 5-15 min (two-step plasma treatment), which is very useful in practical applications. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 160-169, 2014.
Highly Effective Large-Area Inactivation of B. subtilis Spore Using a Planar Dielectric Barrier Discharge Jet With CF4/Air Mixture
(2012-11-01)
Yang, Yi-Wei
;
Wu, Jane-Yii
;
Chiang, Ming-Hung
;
Wu, Jong-Shinn
;
機械工程學系
In this paper, we demonstrate that, by adding a small amount of CF4 (2%) into a compressed-air planar dielectric barrier discharge (DBD), one can effectively inactivate a B. subtilis spore up to 50 mm in width in the postdischarge jet region after ten passes of exposure of plasma plume (equivalent plasma exposure time of 1 s) for treatment distance of up to 14 mm for the spore concentration in the range of 10(5)-10(7) CFU/mL. A possible mechanism is proposed, i.e., the chemically active F atoms and CFn (n = 1-3) are generated through the help of abundant excited nitrogen molecules in the postdischarge region.