Browsing by Author "Yang, Yong-Quan"

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FRET-Created Traffic Light Immunoassay Based on Polymer Dots for PSA Detection
(2020-01-07)
Yang, Yong-Quan
;
Yang, Yu-Chi
;
Liu, Ming-Ho
;
Chan, Yang-Hsiang
;
交大名義發表
There have been enormous efforts for developing the next generations of fluorometric lateral flow immunochromatographic strip (ICTS) owing to the great advances in fluorescent materials in these years. Here we developed one type of fluorometric ICTS based on ultrabright semiconducting polymer dots (Pdots) in which the traffic light-like signals were created by energy transfer depending on the target concentration. This platform was successfully applied for qualitatively rapid screening and quantitatively precise analysis of prostate-specific antigen (PSA) in 10 min from merely one drop of the whole blood sample. This FRET-created traffic light ICTS possesses excellent specificity and an outstanding detection sensitivity of 0.32 ng/mL for PSA. Moreover, we conducted proof-of-concept experiments to demonstrate its potential for multiplexed detection of cancer biomarkers at the same time in an individual test strip by taking advantage of the traffic light signals. To the best of our knowledge, it is the first model of a traffic light-like immunoassay test strip based on Pdots with multiplexing ability. These results would pave an avenue for designing the next generation of point-of-care diagnostics.
Low-toxic cycloplatinated polymer dots with rational design of acceptor comonomers for enhanced photocatalytic efficiency and stability
(2020-07-05)
Chang, Chih-Li
;
Lin, Wei-Cheng
;
Jia, Chuan-Yu
;
Ting, Li-Yu
;
Jayakumar, Jayachandran
By mimicking nature photosynthesis, a library of cycloplatinated polymer dots (Pdots) and pristine Pdots are presented and investigated the importance of selecting acceptor comonomers. A clear design strategy for the selection of acceptor comonomers of Pdots to achieve environmental-friendly, high efficiency, and long stability of photocatalytic reaction can be understood. The active PFTBTA-PtPy Pdots provide the excellent hydrogen evolution rates up to 7.34 +/- 0.82 mmol h(-1) g(-1) under a methanol-free solution and visible-light-driven system. MTT assay experiments confirm the use of the cycloplatinated Pdots as photocatalysts can minimize the toxicity, relative to the conventional approach that directly adding Pt into a solution system. Furthermore, the Pdots can be reactivated for hydrogen generation by the recycling process. The result proves that the use of cycloplatinated Pdots can not only enhance the HER efficiency and stability but also reduce the toxicity.