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Browsing by Author "Yeh, Hsiao-Wei"

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    Measuring the flexibility of immunoglobulin by gold nanoparticles
    (2006-11-08)
    Huang, G. Steven
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    Chen, Yu-Shiun
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    Yeh, Hsiao-Wei
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    材料科學與工程學系奈米科技碩博班
    ;
    奈米科技中心
    We measured the flexibility of Fab and Fc arms of immunoglobulin using gold nanoparticles (GNPs). Enzyme-linked immunosorbent assay was performed to measure the affinity of anti-5 nm GNP antiserum against various sizes of GNPs. The flexibility of Fc was also measured by electron microscopy. The restricted binding affinity indicated that only a very limited amount of freedom was allowed for the Fab-Fab hinge, while Fab-Fc showed a much larger degree of freedom.