Browsing by Author "Yu, Tsai-Jung"

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Microwave-assisted sensing of tetracycline using europium-sensitized luminescence fibers as probes
(2009-11-01)
Kuong, Chi-Lap
;
Yu, Tsai-Jung
;
Chen, Yu-Chie
;
應用化學系
;
Department of Applied Chemistry
In this paper, we describe a facile approach-using silicate fibers immobilized with Eu(III) ions [Eu(III) fibers] as affinity probes-to rapidly sense tetracycline (TC) in complex samples. The fabrication of the Eu(III) fibers is straightforward: Simply immerse a silicate fiber into a glass tube containing Eu(III) and irradiate with microwaves (power, 900 W) for 30 s. The Eu(III) fibers selectively trap TC from aqueous samples via chelation of the beta-diketone functional group of TC with the Eu(III) center. Because the Eu(III)-TC complexes on the fibers are luminescent, as a result of intermolecular energy transfer from the TC moieties to the Eu(III) centers, they can be detected directly using a fluorophotometer. To accelerate the sensing process, we also used microwave irradiation (for only 15 s) to trap the TC molecules from the sample solutions onto the Eu(III) fibers. Furthermore, only a small volume (< 10 mu L) of sample solution is required for these analyses. This approach allows TC to be detected in aqueous samples, with a detection limit of 50 nM.
Multifunctional Fe(3)O(4)/alumina core/shell MNPs as photothermal agents for targeted hyperthermia of nosocomial and antibiotic-resistant bacteria
(2011-10-01)
Yu, Tsai-Jung
;
Li, Po-Han
;
Tseng, Te-Wei
;
Chen, Yu-Chie
;
應用化學系
The appearance of antibiotic-resistant bacterial strains is a serious problem in medical treatment. Thus, it is imperative to explore new therapeutic approaches and antibiotics with which to treat patients suffering from bacterial infections. Materials & Methods: In this work, we propose a targeted hyperthermia therapeutic approach using alumina-coated iron oxide magnetic nanoparticles (Fe(3)O(4)/alumina core/shell MNPs) as photothermal agents to selectively kill bacteria. Results: Fe(3)O(4) MNPs possess photothermal capabilities under near-infrared (NIR) light illumination. The temperature of the MNP suspension (1.33 mu g/mu l, 60 mu l) under illumination with NIR light increased 20 degrees C over 5 min. Functionalization of the surface of the MNPs with an alumina coating allows them to have targeting capability toward bacteria. The prepared Fe(3)O(4)/alumina core/shell MNPs possess several desirable features, including magnetic properties, absorption capability in the NIR region and the ability to target bacteria. The magnetic properties of the Fe(3)O(4)/alumina MNPs allow conjugated target species to aggregate at a specific location under a magnetic field. A NIR laser can then be used to specifically irradiate the aggregated spot to photokill target bacteria. The cell growth of nosocomial bacteria, including Gram-positive, Gram-negative and antibiotic-resistant bacterial strains, can be effectively inhibited by over 95% within 10 min of light irradiation when targeted by Fe(3)O(4)/alumina MNPs. Conclusion: This approach provides a potential therapeutic approach for treating patients suffering from nosocomial and antibiotic-resistant bacterial infections.
Multifunctional Fe3O4/alumina core/shell MNPs as photothermal agents for targeted hyperthermia of nosocomial and antibiotic-resistant bacteria
(2011-10-01)
Yu, Tsai-Jung
;
Li, Po-Han
;
Tseng, Te-Wei
;
Chen, Yu-Chie
;
應用化學系
The appearance of antibiotic-resistant bacterial strains is a serious problem in medical treatment. Thus, it is imperative to explore new therapeutic approaches and antibiotics with which to treat patients suffering from bacterial infections. Materials & Methods: In this work, we propose a targeted hyperthermia therapeutic approach using alumina-coated iron oxide magnetic nanoparticles (Fe3O4/alumina core/shell MNPs) as photothermal agents to selectively kill bacteria. Results: Fe3O4 MNPs possess photothermal capabilities under near-infrared (NIR) light illumination. The temperature of the MNP suspension (1.33 mu g/mu l, 60 mu l) under illumination with NIR light increased 20 degrees C over 5 min. Functionalization of the surface of the MNPs with an alumina coating allows them to have targeting capability toward bacteria. The prepared Fe3O4/alumina core/shell MNPs possess several desirable features, including magnetic properties, absorption capability in the NIR region and the ability to target bacteria. The magnetic properties of the Fe3O4/alumina MNPs allow conjugated target species to aggregate at a specific location under a magnetic field. A NIR laser can then be used to specifically irradiate the aggregated spot to photokill target bacteria. The cell growth of nosocomial bacteria, including Gram-positive, Gram-negative and antibiotic-resistant bacterial strains, can be effectively inhibited by over 95% within 10 min of light irradiation when targeted by Fe3O4/alumina MNPs. Conclusion: This approach provides a potential therapeutic approach for treating patients suffering from nosocomial and antibiotic-resistant bacterial infections.