Browsing by Author "Zhan, Fu-Kai"

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Remarkable influence of alkyl chain lengths on supramolecular hydrogelation of naphthalene diimide-capped dipeptides
(2015-01-01)
Zhan, Fu-Kai
;
Hsu, Shu-Min
;
Cheng, Hsun
;
Lin, Hsin-Chieh
;
材料科學與工程學系
The influence of aliphatic chain lengths on supramolecular hydrogelation of naphthalene diimide-capped dipeptides is described. The dependence of the gelation pH as a function of chain lengths reveals an inverse linear relationship and the gelation pH values cover a broad range from pH 2 to pH 9. In addition, the presence of a n-octyl tail in the hydrogelator could enhance the ability of self-assembly in dilute solution.
Self-Assembled Peptide-Based Hydrogels as Scaffolds for Proliferation and Multi-Differentiation of Mesenchymal Stem Cells
(2017-04-01)
Wang, Yung-Li
;
Lin, Shih-Pei
;
Nelli, Srinivasa Rao
;
Zhan, Fu-Kai
;
Cheng, Hsun
Fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Fmoc-FF) and Fmoc-arginine-glycine-aspartate (Fmoc-RGD) peptides self-assemble to form a 3D network of supramolecular hydrogel (Fmoc-FF/Fmoc-RGD), which provides a nanofibrous network that uniquely presents bioactive ligands at the fiber surface for cell attachment. In the present study, mesenchymal stem cells (MSCs) in Fmoc-FF/Fmoc-RGD hydrogel increase in proliferation and survival compared to those in Fmoc-FF/Fmoc-RGE hydrogel. Moreover, MSCs encapsulated in Fmoc-FF/Fmoc-RGD hydrogel and induced in each defined induction medium undergo in vitro osteogenic, adipogenic, and chondrogenic differentiation. For in vivo differentiation, MSCs encapsulated in hydrogel are induced in each defined medium for one week, followed by injection into gelatin sponges and transplantation into immunodeficient mice for four weeks. MSCs in Fmoc-FF/Fmoc-RGD hydrogel increase in differentiation into osteogenic, adipogenic, and chondrogenic differentiation, compared to those in FmocFF/Fmoc-RGE hydrogel. This study concludes that nanofibers formed by the self-assembly of Fmoc-FF and Fmoc-RGD are suitable for the attachment, proliferation, and multi-differentiation of MSCs, and can be applied in musculoskeletal tissue engineering.
Tumor targeting with DGEA peptide ligands: a new aromatic peptide amphiphile for imaging cancers
(2019-01-25)
Zhan, Fu-Kai
;
Liu, Jyun-Cheng
;
Cheng, Bill
;
Liu, Yen-Chu
;
Lai, Tsung-Sheng
We report a novel fluorescent bioprobe, tetraphenylethylene-Phe-Asp-Gly-Glu-Ala (TPE-FDGEA), and its self-assembly behavior, photophysical properties, and biocompatibility. The hydrogelator TPE-FDGEA exhibited aggregation-induced emission characteristics, which facilitated imaging of PC-3 human prostate cancer cells, thereby demonstrating the utility of such fluorescent probes for specific labeling of target cells in vitro.