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dc.contributor.authorLI Yaw-Kuenen_US
dc.contributor.authorWU Yue-Jinen_US
dc.date.accessioned2014-12-16T06:14:51Z-
dc.date.available2014-12-16T06:14:51Z-
dc.date.issued2014-01-30en_US
dc.identifier.govdocC07K001/14zh_TW
dc.identifier.urihttp://hdl.handle.net/11536/104958-
dc.description.abstractA method for producing a target protein is provided, which includes steps described below. A crude extract including a fusion protein is provided. The fusion protein includes a tag, a target protein and a linker inserted between the tag and the target protein. The fusion protein and magnetic particles are then bound to form a magnetic particle-binding fusion protein. Finally, the linker of the magnetic particle-binding fusion protein undergoes autocleavage by using a cleavage buffer solution to release the target protein. A one-pot process for producing a purified target protein is also provided.zh_TW
dc.language.isozh_TWen_US
dc.titleMETHOD OF PROTEIN PURIFICATIONzh_TW
dc.typePatentsen_US
dc.citation.patentcountryUSAzh_TW
dc.citation.patentnumber20140031531zh_TW
Appears in Collections:Patents


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