標題: | Vectors, methods, systems and kits for protein purification |
作者: | Li Yaw-Kuen Wu Yue-Jin |
公開日期: | 7-八月-2012 |
摘要: | Disclosed herein are autocleaved peptide linkers for producing purified proteins. The autocleaved peptide linkers are inserted between a chitin binding protein (CBP) and a target protein to form a fusion protein. Upon expression of the fusion protein, it is allowed to pass a chitin matrix so that the CBP portion of the fusion protein may be bound with the chitin matrix, the peptide linker then undergoes auto-cleavage in a buffer solution at a pH value of about 5.5-7.5 to release the target protein. The chitin matrix may be regenerated with another buffer solution at a pH value of about 3-4, that is, to release the bound CBP and return to its unbound form. The chitin matrix may be reused for at least 6 times without losing its function. |
官方說明文件#: | C12P021/04 C12N001/20 C12N015/00 |
URI: | http://hdl.handle.net/11536/104562 |
專利國: | USA |
專利號碼: | 08236529 |
顯示於類別: | 專利資料 |