Browsing by Author "Chen, W. L."

Now showing 1 - 8 of 8

A novel two-dimentional gel electrophoresis for studing the cress-linking between beta-Lactoglobulin and milk proteins
(2005)
Chen, W. L.
;
Huang, M. T.
;
Mao, S. J. T.
;
交大名義發表
;
National Chiao Tung University
A novel two-dimentional gel electrophoresis for studing the cress-linking between beta-Lactoglobulin and milk proteins
(2005)
Chen, W. L.
;
Huang, M. T.
;
Mao, S. J. T.
;
交大名義發表
;
National Chiao Tung University
A novel two-dimentional gel electrophoresis for studing the cress-linking between beta-Lactoglobulin and milk proteins
(2005-01-01)
Chen, W. L.
;
Huang, M. T.
;
Mao, S. J. T.
;
交大名義發表
;
National Chiao Tung University
Antioxidant nature of bovine milk beta-lactoglobulin
(2007-02-01)
Liu, H. C.
;
Chen, W. L.
;
Mao, S. J. T.
;
生物科技學系
;
Department of Biological Science and Technology
Heating is necessary for processing milk in the dairy industry, which evidently produces a conformational change in beta-lactoglobulin (beta-LG). beta-Lactoglobulin, a major protein that accounts for approximately 10 to 15% of total milk proteins, is a globular protein consisting of 162 AA with a relative molecular mass of 18.4 kDa. The purpose of the present study was to determine the antioxidant role of beta-LG in milk and the possible mechanism involved. We showed that beta-LG is a mild antioxidant whose potency is less than that of vitamin E and probucol (the latter being an antioxidant used for clinical therapy). The conversion of the beta-LG monomer to dimer was responsible, in part, for the mode of action in protecting low- density lipoproteins against copper-induced oxidation. Cross-linking the free thiol groups of beta-LG by heating (100 degrees C for 2 min), or chemically modifying the beta-LG by carboxymethylation to block the thiol groups resulted in a substantial loss of antioxidant activity. The data suggest that Cys-121 plays an essential role in the antioxidant nature of beta-LG. By using an anti-LG antibody affinity column to deplete the beta-LG from milk, we observed from the lost antioxidant activity that beta-LG contributes approximately 50% of the total activity. Because beta-LG is extremely sensitive to thermal denaturation, to maintain its antioxidant nature, dairy products consumed daily should not be overheated in order to maintain its antioxidant nature.
Distinguish between native and thermally denatured 5-lactoglobulin using a monoclonal antibody as a probe.
(2005)
Mao, S. J. T.
;
Chen, W. L.
;
Yang, M. C.
;
Liu, W. T.
;
交大名義發表
Distinguish between native and thermally denatured beta-lactoglobulin using a monoclonal antibody as a probe
(2005)
Mao, S. J. T.
;
Chen, W. L.
;
Yang, M. C.
;
Liu, W. T.
;
交大名義發表
High-peak-power optically pumped AlGaInAs eye-safe laser at 500-kHz repetition rate with an intracavity diamond heat spreader
(2012-08-01)
Chen, Y. -F.
;
Su, K. W.
;
Chen, W. L.
;
Huang, K. F.
;
Chen, Y. F.
"We report on a compact efficient high-repetition-rate (> 100 kHz) optically pumped AlGaInAs nanosecond eye-safe laser at 1525 nm. A diamond heat spreader bonded to the gain chip is employed to improve the heat removal. At a pump power of 13.3 W, the average output power at a repetition rate 200 kHz is up to 3.12 W, corresponding to a peak output power of 560 W. At a repetition rate 500 kHz, the maximum average power and peak power are found to be 2.32 W and 170 W, respectively."
Pad Characterization for CMOS Technology Using Time Domain Reflectometry
(2008)
Chiu, C. S.
;
Chen, W. L.
;
Liao, K. H.
;
Chen, B. Y.
;
Teng, Y. M.
The pad structure of CMOS technology is characterized by way of time domain reflectometry measurement. Using the on-wafer TDR measurement system, the capacitance of the pad in the CMOS process was extracted and estimated. Measured and Simulated TDR data are also presented in this study. The capacitance is estimated when the curve is fitted by mathematical tool. This method is simple to use, and furthermore the results agree with data extracted from vector network analyzer.