Browsing by Author "Jiang, Yi-Ting"

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Bead-Based Digital Microfluidic Immunoassay for IL-1 beta Detection in Embryo Culture Medium
(2017-01-01)
Tseng, Hsueh-Yang
;
Jiang, Yi-Ting
;
Huang, Cheng-Yeh
;
Lee, Meng-Shiue
;
Hsu, Wensyang
One of the challenges in IVF treatment is to improve the pregnancy rate of selective single embryo implantation. The concentration of IL-1 beta in embryo culture medium may help to predict pregnancy rate. Clinically, each embryo can only get small amount of sample size. However, the traditional immunoassay cannot detect biomarkers in such small sample sizes. Therefore, we combined immunoassay with the bead-based digital microfluidic chip. The chip has two advantages, low sample requirement and low detection limit. We established the mouse IL-1 beta calibration curve, confirm the Human Tubal Fluid (HTF) does not influence the test result and demonstrate that mouse IL-1 beta in clinical mouse embryo culture medium is positively correlate to cultured days. This device has a great potential for increasing IVF pregnancy rates.
Detection of Multiple Embryo Growth Factors by Bead -Based Digital Microfluidic Chip in Embryo Culture Medium
(2018-01-01)
Lee, Chia-Tung
;
Tseng, Hsueh-Yang
;
Jiang, Yi-Ting
;
Haung, Cheng-Yeh
;
Lee, Meng-Shiue
-In IVF treatment, the pregnancy rate of selective single embryo implantation can be improved by detection the concentration of embryo growth factors such as IL -to and TNF-a in embryo culture medium. In this study, we manufacture the high dielectric constant digital microfluidic chip and develop a Bead -based digital microfluidic immunoassay with low sample requirement, low limit of detection and rapid immunoassay. We establish the mouse TNF-a calibration curve and the requirement of sample is 520 nL, limit of detection is 1.55 pg/mL and total immunoassay time is 27 min. We also establish the Human IL -1(3 calibration curve and the requirement of sample is 520 nL, limit of detection is 0.0579 pg/mL and total immunoassay time is 21 min.