Browsing by Author "Lee, Chia-Tung"

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Detection of Multiple Embryo Growth Factors by Bead -Based Digital Microfluidic Chip in Embryo Culture Medium
(2018-01-01)
Lee, Chia-Tung
;
Tseng, Hsueh-Yang
;
Jiang, Yi-Ting
;
Haung, Cheng-Yeh
;
Lee, Meng-Shiue
-In IVF treatment, the pregnancy rate of selective single embryo implantation can be improved by detection the concentration of embryo growth factors such as IL -to and TNF-a in embryo culture medium. In this study, we manufacture the high dielectric constant digital microfluidic chip and develop a Bead -based digital microfluidic immunoassay with low sample requirement, low limit of detection and rapid immunoassay. We establish the mouse TNF-a calibration curve and the requirement of sample is 520 nL, limit of detection is 1.55 pg/mL and total immunoassay time is 27 min. We also establish the Human IL -1(3 calibration curve and the requirement of sample is 520 nL, limit of detection is 0.0579 pg/mL and total immunoassay time is 21 min.
Simultaneous detection of two growth factors from human single-embryo culture medium by a bead-based digital microfluidic chip
(2020-02-15)
Lee, Meng-Shiue
;
Hsu, Wensyang
;
Huang, Hong-Yuan
;
Tseng, Hsueh-Yang
;
Lee, Chia-Tung
The measurement of growth factors released in a culture medium is considered to be an attractive non-invasive approach, apart from the embryo morphology, to identify the condition of an embryo development after fertilization in vitro (IVF), but the available embryo culture medium in the current method is only a few microlitres. This small sample volume, also of small concentration, makes difficult the application of a conventional detection method, such as an enzyme-linked immunosorbent assay (ELISA). A reliable detection of the growth factor from each embryo culture medium of such a small concentration hence remains a challenge. Here for the first time we report the results of measurement of not just one, but two, growth factors, human IL-1 beta and human TNF-alpha, from an individual droplet of embryo culture medium with a bead-based digital microfluidic chip. The required sample volume for a single measurement is only 520 nL; the total duration of the on-chip process is less than 40 min. Using the culture media of human embryos with normal morphologic features, we found that the concentrations of TNF-alpha change little from day 3 to day 5-6, but the concentrations of IL-1 beta for some embryos might double from day 3 to day 5-6. For other embryos even with similar normal morphologic features, some growth factors, such as IL-1 beta, might exhibit different expressions during the culture period. Those growth factors could serve to distinguish the development conditions of each embryo, not merely from an observation of embryo morphology.