Browsing by Issue Date, starting with "2007-06-13"

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Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression
(2007-06-13)
Chang, Shu-Lin
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Chiang, Yi-Ming
;
Chang, Cicero Lee-Tian
;
Yeh, Hsu-Hua
;
Shyur, Lie-Fen
Bidens pilosa is used as an ethnical medicine for bacterial infection or immune modulation in Asia, America and Africa. Here, we employed an IFN-gamma promoter-driven luciferase reporter construct and T cells to characterize immunomodulatory compounds from this plant based on a bioactivity-guided isolation principle. We found that PHA, a positive control, caused a six-fold increase in IFN-gamma promoter activity. In contrast, hot water crude extracts from Bidens pilosa and its butanol subfraction increased IFN-gamma promoter activity to two- and six-fold, respectively. Finally, centaurein (EC50 = 75 mu g/ml) and its aglycone, centaureidin (EC50 = 0.9 mu g/ml), isolated from this butanol subtraction, augmented IFN-gamma promoter activity by similar to four-fold. Consistent with the role of centaurein or its aglycone in IFN-gamma regulation, we showed that centaurein induced the activity of NEAT and NF kappa B enhancers, located within the IFN-gamma promoter, in Jurkat cells. Overall, our results showed that centaurein regulated IFN-gamma transcription, probably via NFAT and NF kappa B in T cells. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
Electroluminescence and electron transport characteristics of aromatic polyimides containing 1,3,4-oxadiazole moiety
(2007-06-13)
Hsu, Shou-Chian
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Whang, Wha-Tzong
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Chao, Ching-Shiun
;
材料科學與工程學系
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Department of Materials Science and Engineering
Single and double layer polymer light-emitting diodes (LEDs) were fabricated by using 2,5-bis(4-aminophenyl)-1,3,4-oxadiazole (BAO)-based aromatic polyimides (APIs). All the resultant APIs have high glass transition temperatures (T-g>250 degrees C) and high thermal decomposition temperatures (T-d>510 degrees C). The APIs exhibit broad fluorescent characteristic, and the fluorescent intensity is related to the intermolecular chain's orientation. BAO-3,3 '-oxydiphthalic anhydride (ODPA) and BAO-4,4 '-(hexafluoro-iso-propylidene) diphthalic anhydride show electroluminescent property in single layer LED devices. By inserting BAO-ODPA in the poly(p-phenylene vinylene)-poly(vinylalcohol) LED device to form a double layer device, the EL efficiency can be improved by two orders of magnitude. (C) 2007 Elsevier B.V. All rights reserved.
Evidence for the electron trap state associated with N-rich clusters in InGaAsN/GaAs quantum wells
(2007-06-13)
Chen, J. F.
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Hsieh, P. C.
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Hsiao, R. S.
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Wang, J. S.
;
Chi, J. Y.
An electron trap state has been found to appear in InGaAsN/GaAs quantum wells ( QWs) as a result of composition fluctuation. Evidence of composition fluctuation is shown by photoluminescence spectra which display an additional low-energy emission due to local regions of N-rich clusters. Enhancing composition fluctuation leads to undulated InGaAsN/GaAs interface with dot-like islands. The capacitance-voltage profiling shows an additional peak following the QW electron ground state peak, suggesting the presence of an electron trap state below the QW electron ground state. The emission properties of this trap state are similar to those of quantum dots immersed in a well. Enhancing composition fluctuation can cause an energy downward shift and spectral broadening of this electron trap state, suggesting an increase in island size and fluctuations of the size and composition. Thus, it is deduced that the electron trap state originated from the N-rich clusters in the well behaving like quantum dots. Upon modulation, the electrons are thermally activated from this trap state to the QW electron ground state and subsequently emitted to the GaAs bottom electrode.
Growth of GaAs expitaxial layers on Si substrate by using a novel GeSi buffer layer
(2007-06-14)
Chang, Edward Y.
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Luo, Guangli
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Yang, Tsung-Hsi
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Chang, Chun-Yen
This invention provides a process for growing Ge epitaixial layers on Si substrate by using ultra-high vacuum chemical vapor deposition (UHVCVD), and subsequently growing a GaAs layer on Ge film of the surface of said Ge epitaixial layers by using metal organic chemical vapor deposition (MOCVD). The process comprises steps of, firstly, pre-cleaning a silicon wafer in a standard cleaning procedure, dipping it with HF solution and prebaking to remove its native oxide layer. Then, growing a high Ge-composition epitaixial layer, such as Si0.1Ge0.9 in a thickness of 0.8 μm on said Si substrate by using ultra-high vacuum chemical vapor deposition under certain conditions. Thus, many dislocations are generated and located near the interface and in the low of part of Si0.1Ge0.9 due to the large mismatch between this layer and Si substrate. Furthermore, a subsequent 0.8 μm Si0.05Ge0.95 layer, and/or optionally a further 0.8 μm Si0.02Ge0.98 layer, are grown. They form strained interfaces of said layers can bend and terminate the propagated upward dislocation very effectively. Therefore, a film of pure Ge is grown on the surface of said epitaixial layers. Finally, a GaAs epitaixial layer is grown on said Ge film by using MOCVD.
Multimedia short message template application system and broadcast system, multimedia short message template applying method and broadcast method
(2007-06-14)
Chen, Deng-Jyi
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Hung, Chi-Chang
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Yang, Po-Chun
A multimedia short message template application system and a broadcast system, a multimedia short message template applying method and a broadcast method are disclosed. The multimedia short message template application system comprises a multimedia object, a multimedia database, a template database and an applying unit. The multimedia object includes an object content attribute and an object motion attribute. The object motion attribute includes an interaction function. The multimedia database is composed of a multimedia data. The template database is composed of short message templates. Any short message template includes a combination arrangement of a multimedia object. A corresponding short message template is found by the applying unit from the template database based on the selection chosen by a user. A corresponding multimedia data is then found from the multimedia database based on the selection chosen by the user again. Lastly, the corresponding multimedia data is loaded into the object content attribute of the short message template within the multimedia object to make a multimedia short message.
俺說
(2007-06-15)
管管
Fabrication of a miniature CMOS-based optical biosensor
(2007-06-15)
Ho, Wei-Jen
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Chen, Jung-Sheng
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Ker, Ming-Dou
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Wu, Tung-Kung
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Wu, Chung-Yu
This work presents a novel, miniature optical biosensor by immobilizing horseradish peroxidase (HRP) or the HRP/glucose oxidase (GOx) coupled enzyme pair on a CMOS photosensing chip with a detection area of 0.5 mm x 0.5 mm. A highly transparent TEOS/PDMS Ormosil is used to encapsulate and immobilize enzymes on the surface of the photosensor. Interestingly, HRP-catalyzed luminol luminescence can be detected in real time on optical H2O2 and glucose biosensors. The minimum reaction volume of the developed optical biosensors is 10 RL. Both optical H2O2 and glucose biosensors have an optimal operation temperature and pH of 20-25 degrees C and pH 8.4, respectively. The linear dynamic range of optical H2O2 and glucose biosensors is 0.05-20 mM H2O2 and 0.5-20 mM glucose, respectively. The miniature optical glucose biosensor also exhibits good reproducibility with a relative standard deviation of 4.3%. Additionally, ascorbic acid and uric acid, two major interfering substances in the serum during electrochemical analysis, cause only slight interference with the fabricated optical glucose biosensor. In conclusion, the CMOS-photodiode-based optical biosensors proposed herein have many advantages, such as a short detection time, a small sample volume requirement, high reproducibility and wide dynamic range. (c) 2007 Published by Elsevier B.V.
俺說
(2007-06-15)
管管
Deep-level emissions in GaAsN/GaAs structures grown by metal organic chemical vapor deposition
(2007-06-15)
Chen, J. F.
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Ke, C. T.
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Hsieh, P. C.
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Chiang, C. H.
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Lee, W. I.
This work presents the deep-level photoluminescence of coherently strained GaAsN/GaAs quantum-well (QW) structures with various GaAsN thicknesses and N contents. A broad deep-level emission at similar to 1.1 eV is observed, whose wavelength is redshifted as the GaAsN thickness increases. Based on its energy separation from the QW emission, this emission is attributed to a transition between the QW electron ground state and a deep level at similar to 0.2 eV above the GaAsN valence-band (VB) edge. This level is shown to be tied to the GaAs band edge. A transition between this level and the GaAs conduction band allows the GaAsN-GaAs band alignment to be evaluated. A type II band lineup is obtained with VB offsets of 0.03 and 0.002 eV for N=0.6% and 1.8%, respectively. The decreased VB offset suggests a transition from type II to type I with increasing N content. Thermal annealing effectively removes this level and improves the QW emission. The concentration of this level is not clearly correlated with N content, suggesting that this level is induced by a low-temperature growth of the GaAsN layer to suppress the composition fluctuation. Given its energy, this level is tentatively assigned to V-Ga. (c) 2007 American Institute of Physics.
俺說
(2007-06-15)
管管
Synthesis and characterization of side-chain liquid crystalline homopolymers and block copolymers containing biphenyl4-ylthiophene and biphenyl-4-ylfluorene pendants
(2007-06-15)
Lee, Kuan-Wei
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Lin, Hong-Cheu
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材料科學與工程學系
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Department of Materials Science and Engineering
A series of new liquid crystalline homopolymers (PI and P2) and block copolymers (P3 and P4) composed of methacrylates containing pendant biphenyl-4-ylthiophene (M1) and biphenyl-4-ylfluorene (M2) units were synthesized by atom transfer radical polymerization (ATRP). The number-average molecular weights (M-n) of the homopolymer (P2) and diblock copolymers (P3 and P4) were in the range of 5153-8713 g mol(-1) with polydispersity indices (PDIs) between 1.17 and 1.25. The thermal, mesogenic, and photoluminescence (PL) properties of all polymers were investigated. Except for the absence of mesogenic properties in block copolymer P4, polymers P1 and P3 possessed the smectic A phase and polymer P2 exhibited the nematic phase. Moreover, the mesomorphism and the layer d-spacing values of the smectic A phase in polymers P1 and P3 were confirmed and characterized by X-ray diffraction (XRD) patterns. (C) 2007 Elsevier Ltd. All rights reserved.
Real-time detection of Escherichia coli O157 : H7 sequences using a circulating-flow system of quartz crystal microbalance
(2007-06-15)
Wu, Vivian C. H.
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Chen, Sz-Hau
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Lin, Chih-Sheng
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生物科技學系
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Department of Biological Science and Technology
A DNA piezoelectric biosensing method for real-time detection of Escherichia coli O157:H7 in a circulating-flow system was developed in this study. Specific probes [a 30-mer oligonucleotide with or without additional 12 deoxythymidine 5'-monophosphate (12-dT)] for the detection of E. coli O157:H7 gene eaeA, synthetic oligonucleotide targets (30 and 104mer) and PCR-amplified DNA fragments from the E. coli O157:H7 eaeA gene (104 bp), were used to evaluate the efficiency of the probe immobilization and hybridization with target DNA in the circulating-flow quartz crystal microbalance (QCM) device. It was found that thiol modification on the 5'-end of the probes was essential for probe immobilization on the gold surface of the QCM device. The addition of 12-dT to the probes as a spacer, significantly enhanced (P < 0.05) the hybridization efficiency (H%). The results indicate that the spacer enhanced the H% by 1.4- and 2-fold when the probes were hybridized with 30- and 104-mer targets, respectively. The spacer reduced steric interference of the support on the hybridization behavior of immobilized oligonucleotides, especially when the probes hybridized with relatively long oligonucleotide targets. The QCM system was also applied in the detection of PCR-amplified DNA from real samples of E. coli O157:H7. The resultant H% of the PCR-amplified double-strand DNA was comparable to that of the synthetic target T-104AS, a single-strand DNA. The piezoelectric biosensing system has potential for further applications. This approach lays the groundwork for incorporating the method into an integrated system for rapid PCR-based DNA analysis. (c) 2006 Elsevier B.V. All rights reserved.
俺說
(2007-06-15)
管管
俺說
(2007-06-15)
管管
俺說
(2007-06-15)
管管
Comparative analysis of two UDP-glucose dehydrogenases in Pseudomonas aeruginosa PAO1
(2007-06-15)
Hung, Ruei-Jiun
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Chien, Han-Sheng
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Lin, Ruei-Zeng
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Lin, Ching-Ting
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Vatsyayan, Jaya
UDP-glucose dehydrogenase (UGDH) catalyzes a two-step NAD(+)-dependent oxidation of UDP-glucose to produce UDPglucuronic acid, which is a common substrate for the biosynthesis of exopolysaccharide. Searching the Pseudomonas aeruginosa PAO1 genome data base for a UGDH has helped identify two open reading frames, PA2022 and PA3559, which may encode a UGDH. To elucidate their enzymatic identity, the two genes were cloned and overexpressed in Escherichia coli, and the recombinant proteins were purified. Both the gene products are active as dimers and are capable of utilizing UDP- glucose as a substrate to generate UDP- glucuronic acid. The Km values of PA2022 and PA3559 for UDP-glucose are similar to 0.1 and 0.4 mM, whereas the K-m values for NAD(+) are 0.5 and 2.0 mM, respectively. Compared with PA3559, PA2022 exhibits broader substrate specificity, utilizing TDP-glucose and UDP-N-acetylglucosamine with one-third the velocity of that with UDP- glucose. The PA2022 mutant and PA2022-PA3559 double mutant, but not the PA3559 mutant, are more susceptible to chloramphenicol, cefotaxime, and ampicillin. The PA3559 mutant, however, shows a reduced resistance to polymyxin B compared with wild type PAO1. Finally, real time PCR analysis indicates that PA3559 is expressed primarily in low concentrations of Mg2+, which contrasts with the constitutive expression of PA2022. Although both the enzymes catalyze the same reaction, their enzymatic properties and gene expression profiles indicate that they play distinct physiological roles in P. aeruginosa, as reflected by different phenotypes displayed by the mutants.
A factoring approach for the Steiner tree problem in undirected networks
(2007-06-15)
Wang, Chu-Fu
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Jan, Rong-Hong
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資訊工程學系
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Department of Computer Science
In communication networks, many applications, such as video on demand and video conferencing, must establish a communications tree that spans a subset K in a vertex set. The source node can then send identical data to all nodes in set K along this tree. This kind of communication is known as multicast communication. A network optimization problem, called the Steiner tree problem (STP), is presented to find a least cost multicasting tree. In this paper, an O(vertical bar E vertical bar) algorithm is presented to find a minimum Steiner tree for series-parallel graphs where vertical bar E vertical bar is the number of edges. Based on this algorithm, we proposed an O(2(2c) . vertical bar E vertical bar) algorithm to solve the Steiner tree problem for general graphs where c is the number of applied factoring procedures. The c value is strongly related to the topology of a given graph. This is quite different from other algorithms with exponential time complexities in vertical bar K vertical bar. (c) 2007 Elsevier Inc. All rights reserved.
Effects of isomeric transformation on characteristics of Alq3 amorphous layers prepared by vacuum deposition at various substrate temperatures
(2007-06-15)
Jian, Zhi-An
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Luo, Ying-Zi
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Chung, Jia-Ming
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Tang, Shiow-Jing
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Kuo, Ming-Chin
Organic tris(8-hydroxyquinoline)aluminum (Alq3) amorphous layers are prepared by vacuum deposition at various substrate temperatures T-sub from 30 to 180 degrees C. The surface morphology and electrical characteristics of these as-deposited layers are studied by atomic force microscopy and current-density versus electric-field (J-E) curves. The temperature dependence of the dark electrical conductivity sigma(T) determined from J-E curves is also examined. These experimental results reveal that the surface and electrical properties of Alq3 amorphous layers deposited at T-sub between 90 and 120 degrees C exhibit an anomalous T-sub dependence. However, this anomalous T-sub dependence is not observed from infrared absorption measurements, and therefore is not the result of chemical degradation. The observed behavior is explained in terms of the property that the vacuum deposition of Alq3 with T-sub between 90 and 120 degrees C involves a thermal interconversion between meridional and facial Alq3 isomers. (c) x 2007 American Institute of Physics.
俺說
(2007-06-15)
管管
多媒體簡訊樣板套用系統及播放系統、多媒體簡訊樣板套用方法及播放方法
(2007-06-16)
陳登吉
;
洪啟彰
;
楊博鈞
本發明係揭露一種多媒體簡訊樣板套用系統及播放系統、多媒體簡訊樣板套用方法及播放方法,該多媒體簡訊樣板套用系統包含多媒體物件、多媒體資料庫、樣板資料庫及套用單元。多媒體物件包含物件內容屬性及物件動作屬性,且物件動作屬性包含一互動功能;多媒體資料庫由多媒體資料所構成;樣板資料庫由簡訊樣板所構成,任一簡訊樣板包含多媒體物件之組合配置;套用單元根據使用者選擇,在樣板資料庫中找出對應之簡訊樣板,再根據使用者選擇,在多媒體資料庫中找出對應之多媒體資料,將其載入簡訊樣板所包含之多媒體物件之物件內容屬性,以建構一多媒體簡訊。