Browsing by Issue Date, starting with "2013-03-19"

Now showing 1 - 20 of 32157

Magnetic Nanoparticle-Based Platform for Characterization of Histidine-Rich Proteins and Peptides
(2013-03-19)
Huang, Shin-Yi
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Chen, Yu-Chie
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應用化學系
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Department of Applied Chemistry
In this study, we developed a platform that can be used to rapidly enrich polyhistidine(His)-tagged proteins/peptides from complex samples selectively using the Fe3O4@Al2O3 magnetic nanoparticles (MNPs) as the affinity probes. At pH 7, the dissociation constant between poly-His, i.e., His(6), and the Fe3O4@Al2O3 MNPs was similar to 10(-5) M and the trapping capacity was similar to 100 nmol/mg for His(6). Enrichment was achieved by vigorously mixing the sample solution (<2 mu L) and the MNPs (1-3 mu g) by pipetting directly onto a matrix-assisted laser desorption/ionization (MALDI) plate for 10 s. The time for the enrichment and the sample volume required for analysis are therefore greatly reduced. After enrichment, the MNP-target species conjugates were promptly isolated by positioning a magnet on the edge of the sample well to aggregate the conjugates into a small spot within similar to 5 so that the nontarget species could be easily removed. Additionally, the problem of finding "sweet spots" on the target species during the MALDI mass spectrometry (MS) analysis was greatly reduced by magnetically isolating the target species on the MALDI plate. The limit of detection for His(6) was, therefore, as low as similar to 400 amol. His(6) and AHHAHHAAD AHHAHHAAD spiked in a protein digest and in human plasma, respectively, were used as the samples to demonstrate the practicability of this approach in selective enrichment of His-rich peptides from complex samples. We also characterized His(6)-tagged proteins enriched on-plate by the Fe3O4@Al2O3 MNPs followed by on-plate tryptic digestion, selective enrichment, and MALDI-MS analysis. This approach can be used to determine quickly whether His(6)-tagged species are present in a sample. In addition, cell lysates containing recombinant Shiga-like toxins tagged with His(6) were used as the samples to further demonstrate that the feasibility of this approach in analyzing very complex samples. The entire analysis process, including the on-plate enrichment and enzymatic digestion followed by MALDI-MS analysis, can be completed within 10 min.
Highly Sensitive Ammonia Sensor with Organic Vertical Nanojunctions for Noninvasive Detection of Hepatic Injury
(2013-03-19)
Dai, Ming-Zhi
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Lin, Yi-Lo
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Lin, Hung-Cheng
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Zan, Hsiao-Wen
;
Chang, Kai-Ting
We successfully demonstrate the first solid-state sensor to have reliable responses to breath ammonia of rat. For thioacetamide (TAA)-induced hepatopathy rats, we observe that the proposed sensor can detect liver that undergoes acute-moderate hepatopathy with a p-value less than 0.05. The proposed sensor is an organic diode with vertical nanojunctions produced by using low-cost colloidal lithography. Its simple structure and low production cost facilitates the development of point-of-care technology. We also anticipate that the study is a starting point for investigating sophisticated breath-ammonia-related disease models.
Electro-optical device
(2013-03-19)
Fan Shih-Kang
;
Chiu Cheng-Pu
An electro-optical device includes a body of fluid, a lower conductor layer disposed below the body of fluid, and a dielectric layer disposed between the body of fluid and the lower conductor layer, connected to the lower conductor layer and supporting the body of fluid. The dielectric layer includes a matrix and a liquid crystal material disposed in the matrix and including liquid crystal molecules. The dielectric layer has a segment disposed adjacent to the fluid. When the segment of the dielectric layer is exposed to an electric field, the body of fluid undergoes electrowetting or dielectrophoresis mechanism, and the orientation of the liquid crystal molecules that are disposed in the segment of the dielectric layer is changed.
Particle charger with sheath air flow for enhancing charging efficiency
(2013-03-19)
Tsai Chuen-Jinn
;
Guan-Yu Lin
;
Hui-Lin Chen
A particle charger with sheath air flow for enhancing charging efficiency includes a housing and a discharge wire. The housing has a charging chamber for accommodating the discharge wire, a particle inlet, a sheath air inlet, an outlet and an accelerating channel. A clean sheath air is guided through the sheath air inlet into the charging chamber to surround charged particles, reducing deposition of charged particles on the inside wall of the housing. A relatively small annular gap of accelerating channel accelerates the charged particles to exit the particle charger, and therefore minimized the particle electrostatic loss due to depositing on the inner surface of the housing. Additionally, uncharged particles approach the discharge wire axially, and charged particles move away radially. It is helpful for the charged particles to diffuse rapidly and uniformly, thereby enhancing the charging efficiency.
Designing an optimal wiring topology for electromigration avoidance
(2013-03-19)
Jiang Hui-Ru
;
Chang Hua-Yu
;
Chang Chih-Long
A method for designing an optimal wiring topology for electromigration avoidance is disclosed. The wiring topology includes multiple sources, multiple sinks and multiple wires. The method includes the following steps: A feasible wire, a wire of the shortest length connecting each pair of source and sink, is calculated, and the capacity of each feasible wire is decided. An initial feasible topology is found. A flow network is created based on the initial topology. Negative cycles are iteratively checked and removed until no more negative cycles.
在有材與廢材之間－從莊子寓言裡「一棵樹」的妙想談起
(2013-03-20)
姚彥淇
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開放教育推動中心
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Open Education Office
課程首頁本課程是由交通大學新文藝復興閱讀計畫提供。 96 年 8 月當各大學紛紛摩拳擦掌準備迎接新生的同時，交通大學校長吳重雨則因為一則來自於七月份泰晤士報，有關於英國首相布朗公布其夏日讀書清單的新聞中，看到了他對知識的尊重。在這同時，吳重雨校長也思考著，要如何為學生準備追求知識的環境和激發他們對知識的尊重與喜愛。台積電張忠謀董事長曾陳述他 18 歲進入哈佛那一年，博覽文史哲理工經典群籍，至此奠定了他求知的基礎，並借海明威名言，將其改為「哈佛這一年為人生可帶走的盛宴。」吳重雨校長以張董事長的學習為借鏡，認為學生固然能藉由自我閱讀的方式學習到豐沛的知識，但透過名人、大師親臨現場地介紹經典書籍，更能加深學子們對於書籍的認識與興趣，這也是「新文藝復興閱讀計劃」 ( 以下簡稱 : 「經典名著選讀」 ) 活動展開籌劃的緣由。於是，吳重雨校長便積極敦請「交大通識教育委員會」，接手籌備本計劃的相關活動，而該中心也成為實際施行本計畫的策動、開課以及認證單位。至此，期許交大學子藉由閱讀、聽講來提昇人文素養、開拓視野的「新文藝復興閱讀計劃」，就此展開。
交大e-News第137期
(2013-03-20)
Secretariat
;
國立交通大學秘書室
功能性奈米粒子抗生素及其製法
(2013-03-21)
陳月枝
;
黃偉杰
本發明係提供一種新穎之功能性奈米粒子抗生素及其製造方法，尤其是一種表面具有抗生素修飾之奈米粒子，以解決臨床上普遍存在之細菌抗藥性之問題。本發明之功能性奈米粒子探針能辨識致病細菌，在近紅外光波長照射下可用為光熱試劑，並有效地抑制細菌之生長。
BONDING METHOD FOR THREE-DIMENSIONAL INTEGRATED CIRCUIT AND THREE-DIMENSIONAL INTEGRATED CIRCUIT THEREOF
(2013-03-21)
CHEN KUAN-NENG
;
Hsu Sheng-Yao
The present invention discloses a bonding method for a three-dimensional integrated circuit and the three-dimensional integrated circuit thereof. The bonding method comprises the steps of: providing a substrate; depositing a film layer on the substrate; providing a light source to light onto the film layer to form a graphic structure; forming a metal co-deposition layer by a first metal and a second metal that are co-deposited on the film layer; providing a first integrated circuit having the substrate, the film layer and the metal co-deposition layer sequentially; providing a second integrated circuit that having the metal co-deposition layer, the film layer and the substrate sequentially; and the first integrated circuit is bonded with the second integrated circuit at a predetermined temperature to form a three-dimensional integrated circuit.
位移量測系統及其方法
(2013-03-21)
徐桂珠
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陳啟昌
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詹佳樺
;
賴暎杰
一種位移量測系統，包括一同調光源、一二維光柵、一光感測器及一訊號處理裝置。當同調光入射至二維光柵後，產生一零階光束及多個一階繞射光束。零階光束與其中的兩個一階繞射光束在光感測器上形成不同方向的干涉條紋。因此，當二維光柵移動時，位移量測系統藉由計算不同方向的干涉條紋之相位差，而獲得二維光柵於對應方向上的位移量。此外，當二維光柵旋轉時，位移量測系統藉由一階繞射光束之繞射圖形的旋轉角度，而獲得二維光柵的旋轉量。
都市縫隙中的少女之美
(2013-03-24)
王宗瑜
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Department of Communication and Technology
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傳播與科技學系
昔日繁華、位於台中市中心的忠信市場，如今成為陰暗破舊的老社區。近年來，藝術家與文創產業的陸續進駐，為沒落的傳統老市集注入一股如少女般的青春甜美與活力。
漫畫的深度
(2013-03-24)
林伯勳
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Department of Communication and Technology
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傳播與科技學系
以生動的圖畫配上淺白的文字，《法庭新鮮人》用有別於以往的方式進行法學教育，書中談到各種常見的法律問題，像是離婚、詐欺、傷害……等等，從民事到刑事無所不包。
從娛樂到競技
(2013-03-24)
顏學廷
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Department of Communication and Technology
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傳播與科技學系
電動玩具是年輕人的玩意？仔細算來，電玩作為遊戲媒介，的確是相當年輕沒錯。
短片幼風潮　兒童玩金穗
(2013-03-24)
吳晉宇
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Department of Communication and Technology
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傳播與科技學系
一般認為，演員的經歷豐不豐富，會影響到在影片中投射給觀眾的感情，但還稱不上多的小孩，為何會開始在影片裡擔任重要角色的呢？
伊斯蘭在台灣
(2013-03-24)
王蒂鷹
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Department of Communication and Technology
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傳播與科技學系
在台灣，信仰伊斯蘭教的人數約為六、七萬，占總人數比例的少數。信仰人口少加上帶有中東色彩的神秘面紗，使得大多數台灣民眾對伊斯蘭教不甚了解。
你今天穿什麼
(2013-03-24)
廖敏廷
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Department of Communication and Technology
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傳播與科技學系
翻開衣櫥，拿出屬於你的特色褲子，不僅實穿又展現性格。決定做自己，為自己的時尚裝扮。
小綠綠的旗幻旅程
(2013-03-24)
郭丹穎
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Department of Communication and Technology
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傳播與科技學系
那是一場，無法忘懷，也難以忘懷的旗幻旅程。
復古年代的流行指標
(2013-03-24)
姜藍茵
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Department of Communication and Technology
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傳播與科技學系
復古的元素不斷被設計師應用，他們不僅從50年代到80年代的流行人物尋找靈感，也擷取當時的流行元素來做創新的設計。究竟，在各個年代經典的流行人物和復古的元素有那些呢?
唱片銷售差　本土最傷
(2013-03-24)
王泓文
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Department of Communication and Technology
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傳播與科技學系
台灣近十年的實體唱片銷售額大量減少，除了受到盜版的衝擊，還有網路音樂的影響，其中以本土唱片的成績最為慘澹。
留或拆　眷村兩難課題
(2013-03-24)
歐士豪
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Department of Communication and Technology
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傳播與科技學系
近年來眷村相關議題不時出現，隨著彩虹眷村的熱潮與寶島一村走紅，眷村在一時之間成為大家眼中的焦點。