Browsing by Issue Date, starting with "2015-06-14"

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另類系籃　文組男孩夢想
(2015-06-14)
許鴻財
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Department of Communication and Technology
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傳播與科技學系
交痛大學客家學院男子籃球隊「院籃」已邁入第十屆，最初創立是為了實現一個打籃球的夢想。
社群網站　吸睛大解密
(2015-06-14)
陳亞柔
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Department of Communication and Technology
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傳播與科技學系
究竟為什麼社群網站沒人追蹤呢？主要原因就在於網站提供的內容。
柔軟布料　訴出瑰麗夢想
(2015-06-14)
陳怡均
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Department of Communication and Technology
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傳播與科技學系
服裝設計師莊承華，善於將無邊際的瑰麗幻想投注於作品之中，並揉合柔美與剛毅特質、透過服裝充分展現女性美。
象棋大師　棋力非凡
(2015-06-14)
許翔
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Department of Communication and Technology
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傳播與科技學系
亞洲象棋大師趙奕帆的故事，對於下棋有許多心路歷程，其中盲棋賽是他最知名的拿手絕活。
視覺饗宴　動畫系畢展
(2015-06-14)
侯怡安
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Department of Communication and Technology
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傳播與科技學系
前陣子適逢畢業季，動畫相關科系成果展也紛紛展出許多畢業作品。
情感教育　不只談情說愛
(2015-06-14)
劉雨婕
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Department of Communication and Technology
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傳播與科技學系
情感是人類與生俱來的能力，但學習如何表達卻是一門深奧的學問。
總編輯的話
(2015-06-14)
顏晟宇
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Department of Communication and Technology
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傳播與科技學系
本期喀報為二一九期，為這學期的最後一篇喀報。共十三篇稿件，有四篇電視專題、三篇新聞圖表、三篇廣播、一篇文字、兩篇動畫，題材種類豐富。
微苦童話　少年冒險之旅
(2015-06-14)
簡品心
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Department of Communication and Technology
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傳播與科技學系
《槍與巧克力》是日本小說家乙一的作品，表面看似少年推理小說，卻暗藏許多社會問題。
療癒商機　從超商到餐廳
(2015-06-14)
李恬芳
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Department of Communication and Technology
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傳播與科技學系
文明社會裡，人們生活壓力大，越來越多的療癒商機趁勢興起。
掌握細節　美味不流失
(2015-06-14)
洪詩宸
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Department of Communication and Technology
;
傳播與科技學系
介紹食材從採買、貯存到烹飪中所需要注意的事項，以及近年流行的超級食物。
政府社福團體　共同助街友
(2015-06-14)
張凱翔
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Department of Communication and Technology
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傳播與科技學系
臺南市政府舉辦街友慶端午活動，讓街友度過溫馨的佳節。同時也有許多非政府組織，為改善街友的生活而努力。
畢業新鮮人　職場學問大
(2015-06-14)
黃馨儀
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Department of Communication and Technology
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傳播與科技學系
恰逢畢業季，二○一五年舉辦「企業最愛大學畢業生調查」，「新鮮人表現超出企業預期」的前五名學校與一般認知的考試排名不盡相同。
承襲精神　日式老建築重生
(2015-06-14)
張婷芳
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Department of Communication and Technology
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傳播與科技學系
辛志平故居建於日治時期，現今承襲校長精神成為附近居民的學習環境。
存在的意義　寄生與被寄生
(2015-06-14)
賴奕安
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Department of Communication and Technology
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傳播與科技學系
在不知名寄生物種的吞噬下，面對天敵的人類該如何應對。
Ablation of androgen receptor gene triggers right ventricular outflow tract ventricular tachycardia
(2015-06-15)
Tsai, Wen-Chin
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Lu, Yen-Yu
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Chen, Yao-Chang
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Chang, Chien-Jung
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Kao, Yu-Hsun
Background: Sex hormones and calcium (Ca2+) regulation play roles in the pathophysiology of ventricular tachycardia from right ventricular outflow tract (RVOT). The purpose of this study was to evaluate whether androgen receptor knockout (ARKO) can increase RVOT arrhythmogenesis through modulating RVOT electrophysiology and Ca2+ homeostasis. Methods: Conventional microelectrodes were used to study the action potential (AP) in RVOT tissues prepared from wild type (WT) and ARKO mice (aged 6-10 months) before and after caffeine (1 mM), isoproterenol (1 mu M), adenosine (10 mu M) and flecainide (5 mu M) administration. The Fluo-3 fluorescence Ca2+ imaging with confocal microscopy and western blots were used to investigate intracellular Ca2+ (Ca-i(2+)) transients, Ca2+ sparks, and the expressions of ionic channel proteins in ARKO and WT RVOT myocytes. Results: We found that ARKO RVOTs (n = 13) had longer AP duration, faster burst firing (5.4 +/- 0.7 vs. 3.4 +/- 0.7 Hz, P < 0.05), and higher incidence of early afterdepolarizations (82% vs. 8%, P < 0.001) than WT RVOTs (n = 11). Adenosine and flecainide can suppress caffeine-or isoproterenol-induced spontaneous rates and burst firing in WT RVOTs, but not in ARKO RVOTs. ARKO RVOT myocytes had a higher frequency (7.7 +/- 2.8 vs. 1.3 +/- 0.4 spark/mm/s, P < 0.05) and incidence (89% vs. 47%, P < 0.05) of Ca2+ sparks, and greater expressions of Cav1.2, NCX, phosphorylated RyR (s2814), phosphorylated phospholamban (Thr17), CAMKII and GRK2 than WT RVOT myocytes. However, ARKO andWT RVOT myocytes exhibit similar Ca-i(2+) transients and SR Ca2+ content, and less expression of calsequestrin. Conclusions: ARKO changes RVOT electrophysiology and Ca2+ homeostasis with increased ventricular arrhythmogenesis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Exploring the influence of high order transverse modes on the temporal dynamics in an optically pumped mode-locked semiconductor disk laser
(2015-06-15)
Tsou, C. H.
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Liang, H. C.
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Wen, C. P.
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Su, K. W.
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Huang, K. F.
We quantitatively investigate the influence of high-order transverse modes on the self-mode locking (SML) in an optically pumped semiconductor laser (OPSL) with a nearly hemispherical cavity. A physical aperture is inserted into the cavity to manipulate the excitation of high-order transverse modes. Experimental measurements reveal that the laser is operated in a well-behaved SML state with the existence of the TEM0,0 mode and the first high-order transverse mode. While more high-order transverse modes are excited, it is found that the pulse train is modulated by more beating frequencies of transverse modes. The temporal behavior becomes the random dynamics when too many high-order transverse modes are excited. We observe that the temporal trace exhibits an intermittent mode-locked state in the absence of high-order transverse modes. (C) 2015 Optical Society of America
Reaction-based epoxide fluorescent probe for in vivo visualization of hydrogen sulfide
(2015-06-15)
Sathyadevi, Palanisamy
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Chen, Yu-Jen
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Wu, Shou-Cheng
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Chen, Yen-Hao
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Wang, Yun-Ming
Hydrogen sulfide (H2S) has emerged as the most important biosynthetic gasotransmitters along with nitric oxide (NO) and carbon monoxide (CO). In this study, we report the design and the synthesis of a new epoxide fluorescent probe 7-glycidyloxy-9-(2-glycidyloxycarbonylphenyl)-2-xanthone (FEPO) for use in in vivo visualization of hydrogen sulfide. The probe employs a fluorescein as a fluorophore, and is equipped with an operating epoxide unit. FEPO functions via epoxide ring opening upon nucleophilic attack of H2S. This ring opening strategy may open a new avenue for the development of various H2S fluorescent sensors. FEPO showed high selectivity and high sensitivity for H2S. FEPO\'s cytotoxicity was tested using MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide) assay. Furthermore, the use of confocal imaging of H2S and in vivo imaging in live zebra fish demonstrated FEPO\'s potential biological applications. We anticipate that, owing to their ideal properties, probes of this type will find great uses in exploring the role of H2S in biology. (C) 2015 Elsevier B.V. All rights reserved.
Label-Free Fluorescent Copper Nanoclusters for Genotyping of Deletion and Duplication of Duchenne Muscular Dystrophy
(2015-06-16)
Chen, Chung-An
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Wang, Chun-Chi
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Jong, Yuh-Jyh
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Wu, Shou-Mei
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生物科技學院
Real applications in clinical diagnosis of label-free fluorescent copper nanoclusters (CuNCs) are demonstrated. Double-strand DNA (dsDNA) can act as an effective template for the formation of CuNCs, which can be used to distinguish the deletion or duplication genotypes of Duchenne muscular dystrophy (DMD) due to different fluorescent intensities. After PCR, the DMD amplicons reacted with copper ion by reduction of ascorbic acid and generated fluorescence. The exons of the DMD gene were taken as the model analytes for genetic diagnosis. In this sensing system, the deletion type does not show fluorescence; on the other hand, the duplication type emits higher fluorescence than normal type. Parameters of this sensing system were optimized, including PCR conditions, levels of copper ion and ascorbate, and reaction time. The DMD-dominated exons 45, 46, and 47 were detected, and the method was applied to six samples of DMD patients. The results were consistent with those of the multiplex ligation-dependent probe amplification method. This strategy was feasible to detect all exons of this disease.
Anchor-based classification and type-C inhibitors for tyrosine kinases
(2015-06-16)
Hsu, Kai-Cheng
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Sung, Tzu-Ying
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Lin, Chih-Ta
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Chiu, Yi-Yuan
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Hsu, John T. -A.
Tyrosine kinases regulate various biological processes and are drug targets for cancers. At present, the design of selective and anti-resistant inhibitors of kinases is an emergent task. Here, we inferred specific site-moiety maps containing two specific anchors to uncover a new binding pocket in the C-terminal hinge region by docking 4,680 kinase inhibitors into 51 protein kinases, and this finding provides an opportunity for the development of kinase inhibitors with high selectivity and anti-drug resistance. We present an anchor-based classification for tyrosine kinases and discover two type-C inhibitors, namely rosmarinic acid (RA) and EGCG, which occupy two and one specific anchors, respectively, by screening 118,759 natural compounds. Our profiling reveals that RA and EGCG selectively inhibit 3% (EGFR and SYK) and 14% of 64 kinases, respectively. According to the guide of our anchor model, we synthesized three RA derivatives with better potency. These type-C inhibitors are able to maintain activities for drug-resistant EGFR and decrease the invasion ability of breast cancer cells. Our results show that the type-C inhibitors occupying a new pocket are promising for cancer treatments due to their kinase selectivity and anti-drug resistance.
High-Performance and Stable Gel-State Dye-Sensitized Solar Cells Using Anodic TiO2 Nanotube Arrays and Polymer-Based Gel Electrolytes
(2015-06-17)
Seidalilir, Zahra
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Malekfar, Rasoul
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Wu, Hui-Ping
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Shiu, Jia-Wei
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Diau, Eric Wei-Guang
Highly ordered and vertically oriented TiO2 nanotube (NT) arrays were synthesized with potentiostatic anodization of Ti foil and applied to fabricate gel-state dye-sensitized solar cells (DSSCs). The open structure of the TiO2 NT facilitates the infiltration of the gel-state electrolyte; their one-dimensional structural feature provides effective charge transport. TiO2 NTs of length L = 15-35 mu m were produced on anodization for periods of t = 5-15 h at a constant voltage of 60 V, and sensitized with N719 for photovoltaic characterization. A commercially available copolymer, poly(methyl methacrylate-co-ethyl acrylate) (PMMA-EA) served as a gelling agent to prepare a polymer-gel electrolyte (PGE) for DSSC applications. The PGE as prepared exhibited a maximum conductivity of 4.58 mS cm(-1) with PMMA-EA (7 wt %). The phase transition temperature (T-p) of the PGE containing PMMA-EA at varied concentrations was determined on the basis of the viscosities measured at varied temperatures. T-p increased with increasing concentration of PMMA-EA. An NT-DSSC with L = 30 mu m assembled using a PGE containing PMMA-EA (7 wt %) exhibited an overall power conversion efficiency (PCE) of 6.9%, which is comparable with that of a corresponding liquid-type device, PCE = 7.1%. Moreover, the gel-state NT-DSSC exhibited excellent thermal and light-soaking enduring stability: the best device retained similar to 90% of its initial efficiency after 1000 h under 1 sun of illumination at 50 degrees C, whereas its liquid-state counterpart decayed appreciably after light soaking for 500 h.