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Browsing by Issue Date, starting with "2017-12-02"

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    Noninvasive prenatal diagnosis of fetal aneuploidy by circulating fetal nucleated red blood cells and extravillous trophoblasts using silicon-based nanostructured microfluidics
    (2017-12-02)
    Huang, Chung-Er
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    Ma, Gwo-Chin
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    Jou, Hei-Jen
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    Lin, Wen-Hsiang
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    Lee, Dong-Jay
    Background: Noninvasive prenatal testing (NIPT) based on cell-free DNA in maternal circulation has been accepted worldwide by the clinical community since 2011 but limitations, such as maternal malignancy and fetoplacental mosaicism, preclude its full replacement of invasive prenatal diagnosis. We present a novel silicon-based nanostructured microfluidics platform named as "Cell Reveal (TM)" to demonstrate the feasibility of capturing circulating fetal nucleated red blood cells (fnRBC) and extravillous cytotrophoblasts (EVT) for cell-based noninvasive prenatal diagnosis (cbNIPD). Methods: The "Cell Reveal (TM)" system is a silicon-based, nanostructured microfluidics using immunoaffinity to capture the trophoblasts and the nucleated RBC (nRBC) with specific antibodies. The automated computer analysis software was used to identify the targeted cells through additional immunostaining of the corresponding antigens. The identified cells were retrieved for whole genome amplification for subsequent investigations by micromanipulation in one microchip, and left in situ for subsequent fluorescence in situ hybridization (FISH) in another microchip. When validation, bloods from pregnant women (n = 24) at gestational age 11-13(+6) weeks were enrolled. When verification, bloods from pregnant women (n = 5) receiving chorionic villus sampling or amniocentesis at gestation age 11(+4)-21 weeks with an aneuploid or euploid fetus were enrolled, followed by genetic analyses using FISH, short tandem repeat (STR) analyses, array comparative genomic hybridization, and next generation sequencing, in which the laboratory is blind to the fetal genetic complement. Results: The numbers of captured targeted cells were 1-44 nRBC/2 ml and 1-32 EVT/2 ml in the validation group. The genetic investigations performed in the verification group confirmed the captured cells to be fetal origin. In every 8 ml of the maternal blood being blindly tested, both fnRBC and EVT were always captured. The numbers of captured fetal cells were 14-22 fnRBC/4 ml and 1-44 EVT/4 ml of maternal blood. Conclusions: This report is one of the first few to verify the capture of fnRBC in addition to EVT. The scalability of our automated system made us one step closer toward the goal of in vitro diagnostics.
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    惰
    (2017-12-03)
    黃昱晉
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    Department of Communication and Technology
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    傳播與科技學系
    懶惰是現代人的通病，卻沒意識到其背後可能帶來的災難。
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    春秋美術社　維護傳統技藝
    (2017-12-03)
    王貞懿
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    Department of Communication and Technology
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    傳播與科技學系
    位於彰化市的春秋美術社，是一間經營五十餘年的老店。店內置放著各式各樣的彩繪燈籠，從剖竹、編織、繃布再到彩繪都是純手工完成，如此技藝現已少見。
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    南機場　桑榆暮景的模範社區
    (2017-12-03)
    鄭頎
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    Department of Communication and Technology
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    傳播與科技學系
    南機場公寓曾為台北市最高級、最現代化的集合住宅，卻隨著光陰走過漸漸凋零。
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    總編輯的話
    (2017-12-03)
    范晨星
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    Department of Communication and Technology
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    傳播與科技學系
    本期為喀報277期，稿件數量共26篇，內容及類目豐富，值得一讀。本期頭題「破除迷思？喝酒對大腦的益處」深入淺出地帶讀者認識酒精對大腦的益處，顛覆過去飲酒對腦部功能只有負面影響的觀念，相當有趣。
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    永不放棄　排球國手楊孟樺
    (2017-12-03)
    王沛軒
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    Department of Communication and Technology
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    傳播與科技學系
    專訪中華台北女排代表隊自由球員楊孟樺，「球不落地，永不放棄」是她對排球的執著與堅持。
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    離鄉背井為了什麼？
    (2017-12-03)
    關子茵
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    Department of Communication and Technology
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    傳播與科技學系
    看看澳門學生來台灣讀書的背後原因。
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    三千隻狗的媽媽　徐雯慧園長
    (2017-12-03)
    李哲君
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    Department of Communication and Technology
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    傳播與科技學系
    臉書粉專徐文良（徐園長護生園）擁有三十五萬名粉絲，是流浪動物收容所的典範，但鮮少人知道創立這一切的幕後推手，她是徐雯慧，徐園長。
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    寄往地獄的一封信
    (2017-12-03)
    葉宜瑩
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    Department of Communication and Technology
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    傳播與科技學系
    就只是想寫封信問問，祢現在還好嗎？
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    破除迷思？喝酒對大腦的益處
    (2017-12-03)
    馮瑜庭
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    Department of Communication and Technology
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    傳播與科技學系
    近期有越來越多研究顛覆過去飲酒對腦部功能只有負面影響的觀念，開始探討酒精對大腦的益處。
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    咖啡培育之地　李子園
    (2017-12-03)
    陳映瑜
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    Department of Communication and Technology
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    傳播與科技學系
    位於台南的175縣道東山咖啡公路上，有許多咖啡館林立。其中，李子園社區以咖啡豆的種植聞名。
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    偶像不能戀愛？禁愛令為何存在
    (2017-12-03)
    巫尹文
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    Department of Communication and Technology
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    傳播與科技學系
    偶像被拍到戀愛中，甚至公布結婚喜訊時，常會讓粉絲集體喊「失戀了！」有些人甚至因此不再支持此偶像，所以許多公司會下達「禁愛令」，但其中卻也有著違反人權的疑慮。究竟偶像能不能夠戀愛呢？
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    MatchPoint　手邊小物成遙控器　
    (2017-12-03)
    徐仟妤
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    Department of Communication and Technology
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    傳播與科技學系
    2017年UIST年會發布的Matchpoint手勢控制技術，將會改變你對於遙控的看法。
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    不想長大
    (2017-12-03)
    蔡翔宇
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    Department of Communication and Technology
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    傳播與科技學系
    長大了，好像也沒什麼特別的。懂得越多，想得越多，受傷越重。 還不如就當個天真的小孩。
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    消失的沙灘　新興網美聖地
    (2017-12-03)
    陳品文
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    Department of Communication and Technology
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    傳播與科技學系
    隨著海灘消失而沒落的頭城濱海森林公園，意外成為新興網美聖地，再現新活力。
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    為什麼大家愛看文創商業展？
    (2017-12-03)
    蔡亞融
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    Department of Communication and Technology
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    傳播與科技學系
    現今越來越多人喜愛逛文創園區的商業展覽，本篇探討為何文創商業型態的展覽如此受歡迎，以及其優點與面臨的疑慮。
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    關於年夜飯這件事
    (2017-12-03)
    劉翊安
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    Department of Communication and Technology
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    傳播與科技學系
    前陣子是西方的感恩節，感恩節之於東方就像是過年一樣，最重要的是吃感恩節大餐，而在東方過年最重要的則是除夕夜的年夜飯⋯⋯
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    長照　罩不罩？
    (2017-12-03)
    簡嘉瑩
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    Department of Communication and Technology
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    傳播與科技學系
    長期照顧2.0目前有人力不足與財源疑慮的問題，是否有望解決並在未來帶給我們更舒適的晚年生活？
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    政圈的權力遊戲　紙牌屋
    (2017-12-03)
    王毓楷
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    Department of Communication and Technology
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    傳播與科技學系
    一場精心策劃的奪權爭位計畫即將畫下句點。
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    食物辨識　預測你的食譜
    (2017-12-03)
    洪儷娟
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    Department of Communication and Technology
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    傳播與科技學系
    介紹MIT所研發食物辨識系統的最新技術，將食物圖像轉換成食譜。