Browsing by Issue Date, starting with "2017-12-17"

Now showing 1 - 20 of 12458

上天入地　霧運算銜接雲到物
(2017-12-17)
范晨星
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Department of Communication and Technology
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傳播與科技學系
因應IoT、5G和AI等資料密集應用需求，霧運算的出現補足了既有系統的不足，達到商業化的服務目的。
厭女？仇男？網路的性別戰爭
(2017-12-17)
徐仟妤
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Department of Communication and Technology
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傳播與科技學系
近年來的網路社群對於性別議題充滿了肅殺之氣，從經濟、社會與心理學角度切入性別論戰的成因。
周育如　讓設計發酵於生活
(2017-12-17)
洪儷娟
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Department of Communication and Technology
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傳播與科技學系
從周育如的酵母開始，讓人產生對都市的黏性，打開我們對設計的觀點。
10233
(2017-12-17)
鄭仰珉
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Department of Communication and Technology
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傳播與科技學系
第一次自己寫詞錄音，表現有點生澀。但是這首歌，只是想向我最初233室友抱怨「為什麼你們都離開啦」。
總編輯的話
(2017-12-17)
黃珮瑄
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Department of Communication and Technology
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傳播與科技學系
本期為喀報279期，稿件共計13篇，以社會議題及文化現象居多，探討不同議題。本期頭題為「通姦除罪化　到底除什麼？」，帶領讀者了解廢除通姦罪的背景及後續發展，期盼讀者真正認識通姦罪。
限時動態　為何令人著迷？
(2017-12-17)
葉宜瑩
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Department of Communication and Technology
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傳播與科技學系
2017年11月底IG推出新功能，讓限時動態可以永存。透過這項與限時動態初衷不同的新功能，來理解為何我們這麼喜歡發布限時動態。
通姦除罪化　到底除什麼？
(2017-12-17)
甘愷璇
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Department of Communication and Technology
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傳播與科技學系
今年五月司法改革國是會議第五分組決定廢除通姦罪，引起各界討論。通姦除罪化，究竟是除掉什麼罪？
胡蘿蔔蛋糕的故事
(2017-12-17)
李哲君
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Department of Communication and Technology
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傳播與科技學系
胡蘿蔔也可以做成蛋糕？
勇於追求夢想　謝松佑
(2017-12-17)
黃昱晉
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Department of Communication and Technology
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傳播與科技學系
謝松佑是台灣電競戰隊Ahq的創始人，Ahq對他不單單只是一支隊伍，同時也乘載著他的夢想。
傳統木屐玩出新風貌　郭宗興
(2017-12-17)
林明慧
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Department of Communication and Technology
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傳播與科技學系
郭宗興為府城百年木屐的第三代傳人，透過傳統技藝與創新思維，讓木屐跳脫老舊的框架，玩出新花樣。
2017世界人權日　喚起世代溝通
(2017-12-17)
劉翊安
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謝瀚陞
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Department of Communication and Technology
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傳播與科技學系
2017台灣世界人權日於新北市景美人權文化園區盛大展開，主題以「人權」、「多元」和「世代溝通」為核心理念。
拿起吉他在客家音樂上放牧
(2017-12-17)
王毓楷
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Department of Communication and Technology
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傳播與科技學系
在獲得第28屆金曲最佳客語歌手獎的提名後，客家音樂的後起之秀曾仲瑋的下一步究竟為何？就讓我們從他如何踏上客家音樂的創作之路談起。
事件屢出　「食」在安心嗎？　
(2017-12-17)
游允彤
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Department of Communication and Technology
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傳播與科技學系
台灣食品風波不斷，如何從體制改善以建立更理想食品安全環境。
何不食兔肉？寵物食用論戰
(2017-12-17)
朱珈漪
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Department of Communication and Technology
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傳播與科技學系
要你把貓狗兔等寵物當作食物吃下肚，你願意嗎？
iTop-Q: an Intelligent Tool for Top-down Proteomics Quantitation Using DYAMOND Algorithm
(2017-12-19)
Chang, Hui-Yin
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Chen, Ching-Tai
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Ko, Chu-Ling
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Chen, Yi-Ju
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Chen, Yu-Ju
Top-down proteomics using liquid chromatogram coupled with mass spectrometry has been increasingly applied for analyzing intact proteins to study genetic variation, alternative splicing, and post-translational modifications (PTMs) of the proteins (proteoforms). However, only a few tools have been developed for charge state deconvolution, monoisotopic/average molecular weight determination and quantitation of proteoforms from LC-MS1 spectra. Though Decon2LS and MASH Suite Pro have been available to provide intraspectrum charge state deconvolution and quantitation, manual processing is still required to quantify proteoforms across multiple MS1 spectra. An automated tool for interspectrum quantitation is a pressing need. Thus, in this paper, we present a user-friendly tool, called iTop-Q (intelligent Top-down Proteomics Quantitation), that automatically performs large-scale proteoform quantitation based on interspectrum abundance in top-down proteomics. Instead of utilizing single spectrum for proteoform quantitation, iTop-Q constructs extracted ion chromatograms (XICs) of possible proteoform peaks across adjacent MS' spectra to calculate abundances for accurate quantitation. Notably, iTop-Q is implemented with a newly proposed algorithm, called DYAMOND, using dynamic programming for charge state deconvolution. In addition, iTop-Qperforms proteoform alignment to support quantitation analysis across replicates/samples. The performance evaluations on an in-house standard data set and a public large-scale yeast lysate data set show that iTop-Q achieves highly accurate quantitation, more consistent quantitation than using intraspectrum quantitation. Furthermore, the DYAMOND algorithm is suitable for high charge state deconvolution and can distinguish shared peaks in coeluting proteoforms. iTop-Qis publicly available for download at http://ms.iis.sinica.edu.tw/COmics/Software_iTop-Q.
Carbon Fiber Ionization Mass Spectrometry for the Analysis of Analytes in Vapor, Liquid, and Solid Phases
(2017-12-19)
Wu, Min-Li
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Chen, Te-Yu
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Chen, Yen-Chun
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Chen, Yu-Chie
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應用化學系
Various ionization methods in mass spectrometry (MS) are available for the analysis of analytes with different properties. Nonetheless, the use of a single ionization method to analyze mixtures containing analytes with different polarities and volatilities in different phases at atmospheric pressure remains a challenge. Exploring an ionization method that can ionize small organics and large biomolecules with different properties for MS analysis is advantageous. Carbon fiber ionization mass spectrometry (CFI-MS), which uses a carbon fiber bundle as the ion source, is useful for the analysis of small organics with low polarities. Voltage needs to be applied on the carbon fiber bundle to initiate corona discharge for ionization of analytes. In this study, we explore the suitability of using CFI-MS in the analysis of analytes in vapor, liquid, and solid phases using a single carbon fiber (length : similar to 1 cm; diameter: similar to 10 mu m) as the ion source. Furthermore direct electric contact on the carbon fiber is not required. We demonstrate that CFI-MS is useful for analyzing not only small and low-polarity organics but also polar biomolecules, such as peptides and proteins. The limits of detection for analytes with high polarities such as dodecyl trimethylammonium bromide and bradykinin are estimated to be similar to 16 and similar to 53 pM, respectively. Ionization mechanisms, including corona discharge and electrospray, are involved in the ionization of analytes with the polarity from low to high. Furthermore, sesame oil containing aromatic volatiles and compounds with different polarities is used as a model sample to demonstrate the capability of the developed ionization method to provide comprehensive chemical information from a complex sample. In addition, the feasibility of using the developed method for quantitative analysis of nonpolar as well as medium and high polarity analytes is also demonstrated. The sensitivity of the developed method toward analytes with high polarity is higher than those with low polarity. The method precision was estimated to be similar to 7.8%.
ESI MS for Microsized Bioparticles
(2017-12-19)
Ozdemir, Abdil
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Lin, Jung-Lee
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Gulfen, Mustafa
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Lai, Szu-Hsueh
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Hsiao, Chun-Jen
An ESI ion trap mass spectrometer was designed for high-throughput and rapid mass analysis of large bioparticles. Mass calibration of the instrument was performed using commercially available polystyrene (PS) microparticles with a size comparable to cancer cells. Different sites of MCF-7 breast cancer cells (8 to 15 mu m) were used in this study. The masses of different cancer cells were measured. This system allows for the analysis of all types of particles.
Concurrent intersection point of magnetization and magnetoconductivity curves in strongly fluctuating superconductors
(2017-12-20)
Chi, Cheng
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Jiang, Xujiang
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Wang, Jiangfan
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Li, Dingping
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Rosenstein, Baruch
The thermal fluctuations contribution to magnetization and magnetoconductivity of type-II layered superconductors is calculated in the framework of the Lawrence-Doniach model. For numerous high-temperature cuprate superconductors, it was discovered that the magnetization dependence on temperature in a wide range of fields exhibits an intersection point at a temperature slightly below Tc. We notice a similar intersection point of the magnetoconductivity curves at the approximate same temperature. The phenomenon is explained by strong (non-Gaussian) thermal fluctuations with interactions treated using a self-consistent theory. All higher Landau levels should be included. Dimensionality of the fluctuations is defined, and the two- to three-dimensional crossover is the key for the existence of intersection points.
Electromigration Mechanism of Failure in Flip-Chip Solder Joints Based on Discrete Void Formation
(2017-12-20)
Chang, Yuan-Wei
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Cheng, Yin
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Helfen, Lukas
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Xu, Feng
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Tian, Tian
In this investigation, SnAgCu and SN100C solders were electromigration (EM) tested, and the 3D laminography imaging technique was employed for in-situ observation of the microstructure evolution during testing. We found that discrete voids nucleate, grow and coalesce along the intermetallic compound/solder interface during EM testing. A systematic analysis yields quantitative information on the number, volume, and growth rate of voids, and the EM parameter of DZ*. We observe that fast intrinsic diffusion in SnAgCu solder causes void growth and coalescence, while in the SN100C solder this coalescence was not significant. To deduce the current density distribution, finite-element models were constructed on the basis of the laminography images. The discrete voids do not change the global current density distribution, but they induce the local current crowding around the voids: this local current crowding enhances the lateral void growth and coalescence. The correlation between the current density and the probability of void formation indicates that a threshold current density exists for the activation of void formation. There is a significant increase in the probability of void formation when the current density exceeds half of the maximum value.
Controlling bottom-up rapid growth of single crystalline gallium nitride nanowires on silicon
(2017-12-20)
Wu, Ko-Li
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Chou, Yi
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Su, Chang-Chou
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Yang, Chih-Chaing
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Lee, Wei-I.
We report single crystalline gallium nitride nanowire growth from Ni and Ni-Au catalysts on silicon using hydride vapor phase epitaxy. The growth takes place rapidly; efficiency in time is higher than the conventional nanowire growth in metal-organic chemical vapor deposition and thin film growth in molecular beam epitaxy. The effects of V/III ratio and carrier gas flow on growth are discussed regarding surface polarity and sticking coefficient of molecules. The nanowires of gallium nitride exhibit excellent crystallinity with smooth and straight morphology and uniform orientation. The growth mechanism follows self-assembly from both catalysts, where Au acts as a protection from etching during growth enabling the growth of ultra-long nanowires. The photoluminescence of such nanowires are adjustable by tuning the growth parameters to achieve blue emission. The practical range of parameters for mass production of such high crystal quality and uniformity of nanowires is suggested.