Browsing by Issue Date, starting with "2018-12-14"

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Measurement of the Electron Antineutrino Oscillation with 1958 Days of Operation at Daya Bay
(2018-12-14)
Adey, D.
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An, F. P.
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Balantekin, A. B.
;
Band, H. R.
;
Bishai, M.
We report a measurement of electron antineutrino oscillation from the Daya Bay Reactor Neutrino Experiment with nearly 4 million reactor (nu) over bar (e) inverse beta decay candidates observed over 1958 days of data collection. The installation of a flash analog-to-digital converter readout system and a special calibration campaign using different source enclosures reduce uncertainties in the absolute energy calibration to less than 0.5% for visible energies larger than 2 MeV. The uncertainty in the cosmogenic Li-9 and He-8 background is reduced from 45% to 30% in the near detectors. A detailed investigation of the spent nuclear fuel history improves its uncertainty from 100% to 30%. Analysis of the relative (nu) over bar (e) rates and energy spectra among detectors yields sin(2)2 theta(13) = 0.0856 +/- 0.0029 and Delta m(32)(2) = (2.471(-0.070)(+0.068)) x 10(-3) eV(2) assuming the normal hierarchy, and Delta m(32)(2) = -(2.575(-0.070)(+0.068)) x 10(-3) eV(2) assuming the inverted hierarchy.
Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of polyprotein G-expressing cell-based microplates
(2018-12-14)
Chen, Yi-Jou
;
Chen, Michael
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Hsieh, Yuan-Chin
;
Su, Yu-Cheng
;
Wang, Chang-Hung
The sensitivity of traditional enzyme-linked immunosorbent assays (ELISAs) is limited by the low binding avidity and heterogeneous orientation of capture antibodies coated on polystyrene-based microplates. Here, we developed a highly sensitive ELISA strategy by fixing poly-protein G-expressing cells on microplates to improve the coating amount and displayed orientation of capture antibodies. One or eight repeated fragment crystallisable (Fc) binding domains of protein G are stably expressed on the surface of BALB/c 3T3 cells (termed 1pG cells or 8pG cells), which then act as highly antibody-trapping microparticles. The 8pG cells showed higher antibody-trapping ability than the 1pG cells did. The antibody-coating amount of the 8pG cell-based microplates was 1.5-23 times and 1.2-6.8 times higher than that of traditional polystyrene-based and commercial protein G-based microplates, respectively. The 8pG cell-based microplates were then applied to an anti-IFN-alpha sandwich ELISA and an anti-CTLA4 competitive ELISA, respectively, and dramatically enhanced their detection sensitivity. Importantly, direct coating unpurified capture antibody produced by mammalian cells did not impair the antigen-capturing function of 8pG cell-based microplates. The 8pG cell-based microplates exhibited a significant improvement in antibody-coating amount and preserved the homogeneous orientation of capture antibodies, making them a potential replacement for traditional microplates in various formats of ELISAs.
Structural insights into the duplex DNA processing of TREX2
(2018-12-14)
Cheng, Hiu-Lo
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Lin, Chun-Ting
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Huang, Kuan-Wei
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Wang, Shuying
;
Lin, Yeh-Tung
The three prime repair exonuclease 2 (TREX2) is an essential 3'- to-5' exonuclease that functions in cell proliferation, genome integrity and skin homeostasis maintenance. The abnormal expression level of TREX2 can result in broken chromosome, increased susceptibility to skin carcinogenesis and Psoriasis. However, the molecular mechanisms of how TREX2 binds and processes its natural substrates, dsDNA or chromosomal DNA, to maintain genome stability remain unclear. In this study, we present four new crystal structures: apo-TREX2, TREX2 in complex with two different dsDNA substrates, and TREX2 in complex with a processed dsDNA product. Analysis of the structures reveals that TREX2 stacks with the 5'-terminal of dsDNA by a Leu20-Pro21-Asn22 cluster for precisely trimming the 3'-overhang. In addition, TREX2 specifically interacts with the nonscissile strand of dsDNA by an alpha-helix-loop region. The unique interaction patterns of the TREX2-dsDNA complex highlight the requirement of long double-stranded region for TREX2 binding and provide evidence of the functional role of TREX2 in processing chromosomal DNA. Moreover, the non-processive property of TREX2 is elucidated by the structure of TREX2-product complex. Our work discloses the first structural basis of the molecular interactions between TREX2 and its substrates and unravels the mechanistic actions of TREX2.
RETRACTION: Growth behavior and microstructure evolution of ZnO nanorods grown on Si in aqueous solution (Retraction of Vol 274, Pg 438, 2004)
(2018-12-15)
Liou, Sz-Chian
;
Hsiao, Chi-Sheng
;
Chen, San-Yuan
;
材料科學與工程學系
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Department of Materials Science and Engineering
Probing algogenic organic matter (AOM) by size-exclusion chromatography to predict AOM-derived disinfection by-product formation
(2018-12-15)
Hua, Lap-Cuong
;
Lin, Jr-Lin
;
Chao, Shu-Ju
;
Huang, Chihpin
;
環境工程研究所
High-performance size exclusion chromatography (HPSEC) coupled with peak-fitting technique was used to probe molecular weight (MW) properties of algogenic organic matter (AOM). The qualitative and quantitative MW information derived was used to predict AOM-derived disinfection by-product (DBP) formation. We resolved overlapping HPSEC chromatograms of all AOM samples into six major peaks with R-2 > 0.996. This study gave significant insight into the HPSEC profiles of AOM, in which resolved peaks A and B (biopolymers) and peak C (humic substances) showed a strong correlation with the formation of carbonaceous-DBPs (C-DBPs). This likely resulted from the abundance of aromatic structures and conjugated C=C double bonds in their chemical nature. Our results also indicated the importance of algal cells, including intra-cellular and cell-bound organic matter, over extra-cellular organic matter as precursors to C-DBP formation. The application of the information extracted from HPSEC profiles associated with the fluorescent components of AOM showed great improvements in the predictability of THMs, HAAs, and C-DBPs with R-2 > 0.7 and p < 0.05. The outcome of this study will significantly benefit effective control of AOM-derived DBP formation by the chlorination of eutrophic waters. (C) 2018 Elsevier B.V. All rights reserved.
RETRACTION: Nucleation and growth behavior of well-aligned ZnO nanorods onorganic substrates in aqueous solutions (Retraction of Vol 283, Pg 141, 2005)
(2018-12-15)
Lin, Chin-Ching
;
Chen, San-Yuan
;
Cheng, Syh-Yuh
;
材料科學與工程學系
;
Department of Materials Science and Engineering
Flexible Linear and Angular Displacement Sensor Based on a Gradient Guided- Mode Resonance Filter
(2018-12-15)
Wang, Yen-Chieh
;
Huang, Cheng-Sheng
;
機械工程學系
;
Department of Mechanical Engineering
This paper reports a method for micrometer-scale linear and angular displacement measurement based on a gradient grating period guided-mode resonance (GGP-GMR) filter. When the filter is mounted on a moving or rotating object, the displacement on the object can be observed through the changes in the resonant wavelength, which are detected by the GGP-GMR filter. In this paper, the GGP-GMR filter comprised grating periods from 250 to 550 nm with 2-nm increments. Each period comprised 100 repeated cycles, resulting in a total length of 6 mm. For linear displacement, we achieved an average sensitivity of 67 nm/mm for a detection range of 6 mm and a theoretical limit of detection of 3 mu m. To demonstrate the measurement of angular displacement, the GGP-GMR filter was attached to a cylinder with a diameter of 8 cm. The experimental results revealed an average sensitivity of 51.7 nm/degrees for a range of 7 degrees and an experimental limit of detection of 0.002 degrees.
Overexpressed Cep170 localizes as acentrosomal puncta along neurites and promotes neurite outgrowth.
(2018-12-15)
Goh, A.
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Huang, H.
;
Hwang, E.
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生物科技學系
;
生物資訊及系統生物研究所
An optogenetic approach to examine the role of cytoplasmic Ran in acentrosomal microtubule nucleation in neurons.
(2018-12-15)
Lo, W.
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Hsu, C.
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Chiu, H.
;
Hwang, E.
;
生物科技學系
一年十二月　今年過後
(2018-12-16)
黃淳妤
;
Department of Communication and Technology
;
傳播與科技學系
一年有十二個月。這一年度過的時光，是因為有你的陪伴，我才能走向明年。
理想與現實　被輕忽的社工風險
(2018-12-16)
陳盈璇
;
Department of Communication and Technology
;
傳播與科技學系
社會工作者以助人為工作，社會卻以此為理所當然，低估他們的專業價值，甚至忽視他們所面臨的職場風險。
觀光景點分身術
(2018-12-16)
陳子與
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Department of Communication and Technology
;
傳播與科技學系
近年，在一個景點爆紅後，就會有人嗅到商機，複製其操作模式，結果全台灣一堆觀光景點鬧雙胞，這該如何是好？
吸引青年返鄉　該如何做起？
(2018-12-16)
姚璇
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Department of Communication and Technology
;
傳播與科技學系
台灣已進入高齡化社會，鄉村勞動力大量流失，為活絡地方必須積極吸引青年返鄉。但現今台灣是否有打造出良好的返鄉創業環境呢？
熊熊遇見你　療癒人心的卡通
(2018-12-16)
廖庭歡
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Department of Communication and Technology
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傳播與科技學系
《熊熊遇見你》的熱潮你跟上了嗎？除了看著三隻熊每天發生有趣的故事，你知道背後隱藏的深意嗎？
用於光體積變化描記圖法之感測系統的自適應性控制器
(2018-12-16)
趙昌博
;
杜哲怡
;
潘伯威
;
吳妍華
一種用於光體積變化描記圖法之感測系統的自適應性控制器，包括多個硬體電路，經組態而用以：接收經處理後的光體積變化描記圖法信號（PPG信號）；判斷經處理後的PPG信號是否滿足需求；若經處理後的PPG信號滿足需求，則輸出PPG信號；以及若經處理後的PPG信號未滿足需求，則調整用以對PPG信號進行放大之放大器的增益或/與調整光源的驅動信號。
建設公司的大隊長　謝詠濬
(2018-12-16)
殷顗霖
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Department of Communication and Technology
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傳播與科技學系
他從建築工人變成公司的老闆，同時不忘回饋社會。多年來的服務，靠的是一顆熱忱的心。
資料驅動的基站管理方法與裝置
(2018-12-16)
王蒞君
;
鄭紹宏
一種基站管理裝置，包括資料接收器、資料處理器與資料配置器。資料接收器用於接收各小型基站所配置管理的多個參數。資料處理器用以對接收的多個參數進行前處理，以清除無用的多個參數，並且根據經前處理後的多個參數計算出至少一效能。資料處理器還用以根據經前處理後的多個參數的至少一者建立效能預測模型。資料處理器更根據效能預測模型的最佳效能決定最佳參數，以根據最佳參數輸出決定結果。資料配置器連接資料處理器，並根據決定結果配置管理各小型基站。
306期總編輯的話
(2018-12-16)
林侑萱
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Department of Communication and Technology
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傳播與科技學系
本期為喀報306期，總計14篇文章。本期頭題為蜜禪的《陸片配額爭議　政治藝術難分割》文章行雲流水，難度適中獲得熱愛喀報的編輯們一致認可，欲知編輯改稿秘辛，請見總編輯的話分曉。
方潔玫　快樂童年的製作人
(2018-12-16)
郭玟妤
;
Department of Communication and Technology
;
傳播與科技學系
一直秉持著「遊戲學習、自然學習」為教育宗旨的喜兒幼兒園，課堂上沒有簿本、沒有英語教學，創辦人方潔玫不怕孩子輸在起跑點，只希望孩子能夠在快樂的環境中長大。
交通違規檢舉　你實名了沒？
(2018-12-16)
李欣秝
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Department of Communication and Technology
;
傳播與科技學系
交通部於日前修正現行法規，將交通違規檢舉改為實名制，期望能解決匿名檢舉的問題。