Browsing by Issue Date, starting with "2020-06-29"

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Highly Sensitive Lead Ion Detection in One Drop of Human Whole Blood Using Impedance-Modulated Field-Effect Transistors and a Portable Measurement Device
(2020-06-29)
Wang, Shin-Li
;
Hsieh, Ching-Yen
;
Sukesan, Revathi
;
Chen, Jung-Chih
;
Wang, Yu-Lin
This study reports the development of a rapid heavy metal ion screening tool for whole blood samples. The test system consists of an ion selective membrane-based impedance modulated field effect transistor (FET) sensor and a portable sensor measurement unit. This study focuses on the direct detection of lead ions in whole blood, without extensive sample pre-treatments. The sensing methodology is based on impedance changes in the membrane due to the specific ionophore-target metal ion interaction in the presence of a strong electric field. The changes in impedance caused by heavy metal ions are amplified by the FET to obtain a highly sensitive lead ion detection, with very low detection limit (near 10(-11) similar to 10(-10)M Pb2+). To reduce the complexity and enhance sensor performance, the whole blood samples are fractionated on chip without any external actuation/automation using simple gravitational blood cell separation. The test results in PBS, human serum and whole blood samples demonstrate high sensitivity and wide dynamic range of lead ion detection. The miniaturized extended gate FET sensor system is ideal for point of care and home-care diagnostics for heavy metal ion in blood.
Potential enhancement of host immunity and anti-tumor efficacy of nanoscale curcumin and resveratrol in colorectal cancers by modulated electro- hyperthermia
(2020-06-29)
Kuo, I-Ming
;
Lee, Jih-Jong
;
Wang, Yu-Shan
;
Chiang, Hsin-Chien
;
Huang, Cheng-Chung
BackgroundModulated electro-hyperthermia (mEHT) is a form of hyperthermia used in cancer treatment. mEHT has demonstrated the ability to activate host immunity by inducing the release of heat shock proteins, triggering apoptosis, and destroying the integrity of cell membranes to enhance cellular uptake of chemo-drugs in tumor cells. Both curcumin and resveratrol are phytochemicals that function as effective antioxidants, immune activators, and potential inhibitors of tumor development. However, poor bioavailability is a major obstacle for use in clinical cancer treatment.MethodsThis purpose of this study was to investigate whether mEHT can increase anti-cancer efficacy of nanosized curcumin and resveratrol in in vitro and in vivo models. The in vitro study included cell proliferation assay, cell cycle, and apoptosis analysis. Serum concentration was analyzed for the absorption of curcumin and resveratrol in SD rat model. The in vivo CT26/BALB/c animal tumor model was used for validating the safety, tumor growth curve, and immune cell infiltration within tumor tissues after combined mEHT/curcumin/resveratrol treatment.ResultsThe results indicate co-treatment of mEHT with nano-curcumin and resveratrol significantly induced cell cycle arrest and apoptosis of CT26 cells. The serum concentrations of curcumin and resveratrol were significantly elevated when mEHT was applied. The combination also inhibited the growth of CT26 colon cancer by inducing apoptosis and HSP70 expression of tumor cells while recruiting CD3+ T-cells and F4/80+ macrophages.ConclusionsThe results of this study have suggested that this natural, non-toxic compound can be an effective anti-tumor strategy for clinical cancer therapy. mEHT can enable cellular uptake of potential anti-tumor materials and create a favorable tumor microenvironment for an immunological chain reaction that improves the success of combined treatments of curcumin and resveratrol.
A site-moiety map and virtual screening approach for discovery of novel 5-LOX inhibitors
(2020-06-29)
Hsu, Kai-Cheng
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HuangFu, Wei-Chun
;
Lin, Tony Eight
;
Chao, Min-Wu
;
Sung, Tzu-Ying
The immune system works in conjunction with inflammation. Excessive inflammation underlies various human diseases, such as asthma, diabetes and heart disease. Previous studies found that 5-lipoxygenase (5-LOX) plays a crucial role in metabolizing arachidonic acid into inflammatory mediators and is a potential therapeutic target. In this study, we performed an in silico approach to establish a site-moiety map (SiMMap) to screen for new 5-LOX inhibitors. The map is composed of several anchors that contain key residues, moiety preferences, and their interaction types (i.e., electrostatic (E), hydrogen-bonding (H), and van der Waals (V) interactions) within the catalytic site. In total, we identified one EH, one H, and five V anchors, within the 5-LOX catalytic site. Based on the SiMMap, three 5-LOX inhibitors (YS1, YS2, and YS3) were identified. An enzyme-based assay validated inhibitory activity of YS1, YS2, and YS3 against 5-LOX with an IC50 value of 2.7, 4.2, and 5.3 mu M, respectively. All three inhibitors significantly decrease LPS-induced TNF-alpha and IL-6 production, which suggests its potential use an anti-inflammatory agent. In addition, the identified 5-LOX inhibitors contain a novel scaffold. The discovery of these inhibitors presents an opportunity for designing specific anti-inflammatory drugs.
Removal of an abluminal lining improves decellularization of human umbilical arteries
(2020-06-29)
Tuan-Mu, Ho-Yi
;
Chang, Yi-Hao
;
Hu, Jin-Jia
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機械工程學系
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Department of Mechanical Engineering
The decellularization of long segments of tubular tissues such as blood vessels may be improved by perfusing decellularization solution into their lumen. Particularly, transmural flow that may be introduced by the perfusion, if any, is beneficial to removing immunogenic cellular components in the vessel wall. When human umbilical arteries (HUAs) were perfused at a transmural pressure, however, very little transmural flow was observed. We hypothesized that a watertight lining at the abluminal surface of HUAs hampered the transmural flow and tested the hypothesis by subjecting the abluminal surface to enzyme digestion. Specifically, a highly viscous collagenase solution was applied onto the surface, thereby restricting the digestion to the surface. The localized digestion resulted in a water-permeable vessel without damaging the vessel wall. The presence of the abluminal lining and its successful removal were also supported by evidence from SEM, TEM, and mechanical testing. The collagenase-treated HUAs were decellularized with 1% sodium dodecyl sulfate (SDS) solution under either rotary agitation, simple perfusion, or pressurized perfusion. Regardless of decellularization conditions, the decellularization of HUAs was significantly enhanced after the abluminal lining removal. Particularly, complete removal of DNA was accomplished in 24 h by pressurized perfusion of the SDS solution. We conclude that the removal of the abluminal lining can improve the perfusion-assisted decellularization.
自信揚帆～身心障礙者就業服務資源介紹
(國立陽明大學, 2020-06-30)
劉容孜
;
陽明電子報編輯小組
「摺」我的校門，拼一個回憶
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
今年是陽明大學45周年校慶，校友中心特別製作陽明校門紙模型紀念品，在校慶典禮致贈與會貴賓及校友。這次剛好有機會參與到整個設...
【校內商店暑假營業時間】
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
惠蓀咖啡：8:00-18:00（7月6日至8月31日） OK超
An Augmented Reality Head-Up Display System with a Wide-View Eyebox
(2020-06-30)
Wu, Pei-Jung
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Chuang, Chih-Hao
;
Chen, Chien-Yu
;
Wu, Jeng-Han
;
Lin, Bor-Shyh
This study proposes to apply the combination of two 90-degree prisms and a holographic optical element to an image-dividing element, divide an image into three through the virtual image projection system, and accurately project the images to 160 cm behind the windshield. In order to distinguish the left image, middle image, and right image at the horizontal direction, the 90-degree prism is first utilized for horizontally deflecting the light, a holographic optical element is then used for presenting the vertical deflection of the image, and finally, the images are horizontally arranged as a real image on the diffuser. An image-dividing element is eventually integrated to the virtual image projection system. Then, the divided images are made as a virtual image which is projected to 160 cm behind the windshield to combine with the street scene. The horizontal angle of view of an eyebox is increased from 2.51 degrees to 7.22 degrees, and the size of the eyebox is 31.68 cm x 12.48 cm, allowing a wide-view laser-based head-up display with the large-angle beam-splitting function being successfully designed. It would not affect the field of view on the road, and this system could reduce the space and be arranged easily in a vehicle. Therefore, this proposed system is suitable for the aftermarket.
完成未竟的遺憾，田知學校友17年後再撥穗、一圓當年夢
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
今年的畢業典禮，陽明特別邀請振興醫院急診重症醫學部主任田知學校友出席。田校友是陽明醫學系92級畢業生，十七年前因遭逢SARS未
藥理所張婷婷、蔡秉興博士榮獲「科技部博後學術研究獎」
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
本校醫學院藥理學研究所張婷婷博士與蔡秉興博士，分別以糖尿病與基因治療方面的創新研究成果，榮獲科技部「108年度博士後研究人員.
齊賀母校校慶—校友回娘家
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
5月31日，在這個艷陽高照的星期天，45周年校慶展開多場熱鬧有趣的活動，更是一年一度、第19屆「校友回娘家」的大日子！雖受疫情影
【周成功教授－生命科學講座】
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
經歷了長期背誦、記憶和應付考試的大學新生，進入大學後最重要.
We are family & 陽明人
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
自1月21日至3月14日止，由校友中心承辦，邀請本校所有畢業校友、教師、行政同仁、學生，參與「We are family 陽明人」合照徵求活.
那年我們一起走過—五大約會景點
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
自1月21日至3月14日白色情人節為止，由校友中心承辦，邀請陽明校友們，參與「我的約會地點票選陽明5大約會地點」線上投票活動，由
再見隊友、情誼永久—桌球校友盃球賽
(國立陽明大學, 2020-06-30)
蔡欣妤
;
陽明電子報編輯小組
2020年6月7日的早晨，是一年一度的校友盃。今年為了防範疫情，特別增設入場量體溫的環節。看著熟悉的面孔陸續出現在眼前，來為我們加油打氣，好不感動。
A simple strategy to enhance the speed of protein secondary structure prediction without sacrificing accuracy
(2020-06-30)
Juan, Sheng-Hung
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Chen, Teng-Ruei
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Lo, Wei-Cheng
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生物科技學系
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生物資訊及系統生物研究所
The secondary structure prediction of proteins is a classic topic of computational structural biology with a variety of applications. During the past decade, the accuracy of prediction achieved by state-of-the-art algorithms has been >80%; meanwhile, the time cost of prediction increased rapidly because of the exponential growth of fundamental protein sequence data. Based on literature studies and preliminary observations on the relationships between the size/homology of the fundamental protein dataset and the speed/accuracy of predictions, we raised two hypotheses that might be helpful to determine the main influence factors of the efficiency of secondary structure prediction. Experimental results of size and homology reductions of the fundamental protein dataset supported those hypotheses. They revealed that shrinking the size of the dataset could substantially cut down the time cost of prediction with a slight decrease of accuracy, which could be increased on the contrary by homology reduction of the dataset. Moreover, the Shannon information entropy could be applied to explain how accuracy was influenced by the size and homology of the dataset. Based on these findings, we proposed that a proper combination of size and homology reductions of the protein dataset could speed up the secondary structure prediction while preserving the high accuracy of state-of-the-art algorithms. Testing the proposed strategy with the fundamental protein dataset of the year 2018 provided by the Universal Protein Resource, the speed of prediction was enhanced over 20 folds while all accuracy measures remained equivalently high. These findings are supposed helpful for improving the efficiency of researches and applications depending on the secondary structure prediction of proteins. To make future implementations of the proposed strategy easy, we have established a database of size and homology reduced protein datasets at http://10.life.nctu.etu.tw/UniRefNR.
【第18屆傑出校友遴選開始受理推薦】
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
本校自民國93年起開始辦理傑出校友選拔，現已邁入第18屆，敬邀各界踴躍推薦！讓我們看到各行各業服務的校友們努力和成就
榮耀時刻—第17屆傑出校友頒獎
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
本校第17屆傑出校友頒獎典禮於5月31日的校慶典禮中圓滿完成，獲選者蔡錦華、顏鴻順、賈淑麗、林故廷、劉瑞玲、劉靜怡及謝景祥7位
【109年共同暑休日】
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
7月17日起至8月21日，每週五（7/17、7/24、7/31、8/7、8/14、8/.
【2020中學教師生命科學研習營】
(國立陽明大學, 2020-06-30)
陽明電子報編輯小組
今年研習的課程主題為生物細胞通訊的語言，學員將瞭解不同系統.