超高靈敏度、無須標記且即時檢測腸病毒之奈米線場效電晶體生物感測器

Abstract

本計畫之目的為開發一套具有超高靈敏度、可即時多重偵測、操作簡便、並可結合台灣現有的半導體技術來大量生產的腸病毒奈米線場效電晶體生物感測器。實施方法為結合生物分子檢測技術及生物奈米半導體感測器，以腸病毒檢測與監控為標的，應用創新的矽奈米線場效電晶體生物分子感測及以積體電路設計之場效電晶體為基礎，開發分子檢測平台及監控系統。本計畫整合交通大學、國家奈米元件實驗室、儀器科技研究中心及中國醫學大學附設醫院之研究團隊共同執行，並與衛生署疾病管制局研究檢驗中心密切合作。專長與技術整體分工合作架構如下：交通大學研發電子元件與生醫感測之介面整合，建立腸病毒檢測方法並協調各研發單位整體進行之步調；國家奈米元件實驗室負責發展矽奈米線場效電晶體之元件設計、製造、與量測技術，並進行下一代奈米線場效電晶體之研發，目標為達成符合產業應用之矽奈米線場效電晶體生醫感測晶片；儀器科技研究中心將建立從半導體晶片到生物量測系統之微型儀器化關鍵技術，並將各關鍵元件模組化，包括有系統電源供應器模組、微流體生物晶片輸入模組、微流道晶片模組、專一性分子辨視模組、電訊號輸出SOC晶片模組、LCD螢幕顯示數據模組及微流管道後端出口模組之製作，同時將完成原型機模組整合、構形設計製造及系統性能測試，最終目標為開發可攜式之生醫感測儀器。參與腸病毒重症醫療網之中國醫學大學附設醫院將提供臨床應用之需求，並驗證上述研發成果於臨床應用之價值。預期達成之分年計畫目標為第一年：建構超高靈敏度、無須標記且即時之奈米線場效電晶體腸病毒檢測電子晶片系統。本年度將同時考慮臨床實際應用的功能、規格要求及檢測系統規範，於晶片設計時，即徵詢醫師與臨床醫學研究人員之意見。第二年：開發以奈米線場效電晶體之可攜式腸病毒檢測儀器系統，並於畫執行過程中將該檢測系統之確效及查驗登記所需之驗證資料納入規劃。第三年：奈米線場效電晶體腸病毒檢測系統之臨床驗證。

The goal of this proposal is to develop an ultrahigh sensitive and easy to use biosensor for Enterovirus detection. Nanowire field effect transistor will be used as transducer for the biosensing device. A multidisciplinary team including researches from National Chiao Tung University, National Nano Device Laboratories, Instrument Technology Research Center and China Medical University Hospital will work together for integrating a semiconductor chip into an Enterovirus sensing instrument. National Chiao Tung University will be responsible for the interface between electronic device and biomolecule and the coordination among research teams. National Nano Device Laboratories will prepare and characterize electric properties of the nanowire field effect transistor and will further design the next generation device. Instrument Technology Research Center will develop key technologies required for the assemble of an instrument for Enterovirus detection from semiconductor chips and prepare a prototyped instrument. China Medical University Hospital will use the established tools for clinical determination of Enterovirus infection. Tentative goals for each year of the three-year proposal will be as following. In the first year, the nanowire field effect transistor chip system for ultrahigh-sensitive, label-free and real-time detection of Enterovirus will be established. The instrumentation based on the chip system will be developed in the second year. In the third year, the instrument will be tested clinically for Enterovirus detection.