標題: | Monitoring Biomolecular Interaction with 1-D Plasmonic Nanostructure by Using Dark Field Microscopy |
作者: | Lu, Hui-Hisn Lin, Chii-Wann Fang, Yueh-Yuan Hsiao, Tzu-Chien Hsu, Su-Ming 分子醫學與生物工程研究所 Institute of Molecular Medicine and Bioengineering |
關鍵字: | one-dimensional nanostrusture;surface Plasmon;dark field microscopy |
公開日期: | 2009 |
摘要: | This study presents an imaging technique combining multi-functional dark field microscopy (DFM) with spectroscopy to image the nanostructures of different formats of optical disks and identify their optical properties by absorption spectra. The resolution of this DFM system is determined using one-dimension nanostructure with lengths from 160 nm to 600 nm. The pattern sizes are confirmed by atomic force microscopy (AFM). The results show that DFM resolution is about 160 nm with the current setup, and the resulting DFM image size appears to be slightly larger than a AFM image. We then deposited a 50 nm gold film on top of these nanostructures and measured their absorption spectra to identify the relationship between surface plasmon resonance (SPR) spectra and one-dimensional nanostructures. The immobilization of streptavidin on the surface of the gold-coated nanostructure causes changes in the absorption spectrum ranging from 450nm to 650nm. |
URI: | http://hdl.handle.net/11536/134971 |
ISBN: | 978-3-540-89207-6 |
ISSN: | 1680-0737 |
期刊: | 4TH EUROPEAN CONFERENCE OF THE INTERNATIONAL FEDERATION FOR MEDICAL AND BIOLOGICAL ENGINEERING |
Volume: | 22 |
Issue: | 1-3 |
起始頁: | 2300 |
結束頁: | 2303 |
顯示於類別: | 會議論文 |