標題: | Development of High-kappa Eu2Ti2O7 EIS Devices Using Poly-N-isopropylacrylamide as an Enzyme Encapsulation Material for pH Detection and Uric Acid Biosensing |
作者: | Pan, Tung-Ming Chang, Kung-Yuan Wu, Min-Hsien Tsai, Shiao-Wen Ko, Fu-Hsiang Chi, Lifeng 材料科學與工程學系奈米科技碩博班 Graduate Program of Nanotechnology , Department of Materials Science and Engineering |
關鍵字: | Eu2Ti2O7;pH sensitivity;electrolyte-insulator-semiconductor (EIS);poly-N-isopropylacrylamide (PNIPAAm);uric acid |
公開日期: | 1-一月-2012 |
摘要: | In this study, we proposed an electrolyte-insulator-semiconductor (EIS) device fabricating a high-kappa Eu2Ti2O7 sensing membrane deposited on Si substrates through cosputtering for pH detection and uric acid biosensing applications. The EIS device incorporating a high-kappa Eu2Ti2O7 sensing membrane annealed at 900 degrees C exhibited a higher sensitivity of 59.03 mV/pH, a smaller hysteresis voltage of 2.8 mV, and a lower drift rate of 0.586 mV/h compared to those of the other annealing temperatures. These results are attributed to the small number of crystal defects and the large surface roughness. Furthermore, for high-kappa Eu2Ti2O7 EIS-based biosensing application, a phase tunable poly-N-isopropylacrylamide (PNIPAAm) was used as an enzyme encapsulating material, making the loading and removal of a biological recognition element on a biosensor easily. In this study, a hybrid configuration of the high-kappa Eu2Ti2O7 EIS biosensor with an unease-immobilizing PNIPAAm film attached was established and was demonstrated for uric acid biosensing. The sensitivity of a Eu2Ti2O7 EIS biosensor was 17.6 mV/mM for uric acid concentrations ranging from 2 to 8 mM. |
URI: | http://hdl.handle.net/11536/146335 |
ISSN: | 1680-0737 |
期刊: | 5TH EUROPEAN CONFERENCE OF THE INTERNATIONAL FEDERATION FOR MEDICAL AND BIOLOGICAL ENGINEERING, PTS 1 AND 2 |
Volume: | 37 |
起始頁: | 992 |
顯示於類別: | 會議論文 |