標題: | Uncoiling mechanism of Klebsiella pneumoniae type 3 pili measured by using optical tweezers |
作者: | Chen, Feng-Jung Chan, Chia-Han Liu, Kuo-Liang Huang, Ying-Jung Peng, Hwei-Ling Chang, Hwan-You Yew, Tri-Rung Hsu, Ken Y. Hsu, Long 材料科學與工程學系 生物科技學系 分子醫學與生物工程研究所 電子物理學系 光電工程學系 光電工程研究所 Department of Materials Science and Engineering Department of Biological Science and Technology Institute of Molecular Medicine and Bioengineering Department of Electrophysics Department of Photonics Institute of EO Enginerring |
關鍵字: | type 3 pili;optical tweezers;mechanical properties;uncoiling force |
公開日期: | 1-一月-2007 |
摘要: | Pili are bacterial appendages that play many important roles in bacterial behaviors, physiology and interaction with hosts. Via pili, bacteria are able to adhere to, migrate onto, and colonize on host cells, mechanically. Different from the most studied type I and P type pili, which are rigid and thick with an average of 6 similar to 7 nm in diameter, type 3 pili are relatively tiny (3-5 nm in diameter) and flexible, and their biophysical properties remains unclear. By using optical tweezers, we found that the elongation processes of type 3 pili are divided into three phases: (1) elastic elongation, (2) uncoiling-elongation, and (3) intrinsic elongation, separately. Besides, the uncoiling force of the recombinant pili displayed on the surface of E. coli [pmrkABCD(VI)F] is measured 20 pN in average stronger than that of E. coli [pmrkABCD(VI)]. This suggests that pilin MrkF is involved in determining the mechanical properties of the type 3 pili. |
URI: | http://dx.doi.org/10.1117/12.733097 http://hdl.handle.net/11536/146600 |
ISBN: | 978-0-8194-6792-8 |
ISSN: | 0277-786X |
DOI: | 10.1117/12.733097 |
期刊: | OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION IV |
Volume: | 6644 |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 會議論文 |