標題: Improvement of axial excitation confinement in temporal focusing-based multiphoton microscopy via spatially modulated illumination
作者: Chang, Chia-Yuan
Chen, Shean-Jen
光電學院
College of Photonics
關鍵字: Nonlinear microscopy;Three-dimensional microscopy;Multiphoton processes;Temporal focusing;digital micromirror device
公開日期: 1-一月-2017
摘要: Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement (AEC) of a few microns. Herein, a developed TFMPEM with a digital micromirror device (DMD), acting as the blazed grating for light spatial dispersion and simultaneous patterned illumination, has been extended to implement spatially modulated illumination at structured frequency and orientation. By implementing the spatially modulated illumination, the beam coverage at the back-focal aperture of the objective lens can be increased. As a result, the AEC can be condensed from 3.0 mu m to 1.5 mu m in full width at half maximum for a 2-fold enhancement. Furthermore, by using HiLo microscopy with two structured illuminations at the same spatial frequency but different orientation, biotissue images according to the structured illumination with condensed AEC is obviously superior in contrast and scattering suppression.
URI: http://dx.doi.org/10.1117/12.2251183
http://hdl.handle.net/11536/146674
ISBN: 978-1-5106-0675-3; 978-1-5106-0676-0
ISSN: 0277-786X
DOI: 10.1117/12.2251183
期刊: EMERGING DIGITAL MICROMIRROR DEVICE BASED SYSTEMS AND APPLICATIONS IX
Volume: 10117
起始頁: 0
結束頁: 0
顯示於類別:會議論文


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