標題: | Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy |
作者: | Chien, C. C. Tseng, P. Y. Chen, H. H. Hua, T. E. Chen, S. T. Chen, Y. Y. Leng, W. H. Wang, C. H. Hwu, Y. Yin, G. C. Liang, K. S. Chen, F. R. Chu, Y. S. Yeh, H. I. Yang, Y. C. Yang, C. S. Zhang, G. L. Je, J. H. Margaritondo, G. 電子物理學系 Department of Electrophysics |
關鍵字: | X-ray microscopy;Phase contrast radiology;Fresnel phase zone plate;Subcellular organelle |
公開日期: | 1-五月-2013 |
摘要: | Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research. (C) 2012 Elsevier Inc. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.biotechadv.2012.04.005 http://hdl.handle.net/11536/21505 |
ISSN: | 0734-9750 |
DOI: | 10.1016/j.biotechadv.2012.04.005 |
期刊: | BIOTECHNOLOGY ADVANCES |
Volume: | 31 |
Issue: | 3 |
起始頁: | 375 |
結束頁: | 386 |
顯示於類別: | 會議論文 |