Catalytic Behaviors in Modulating Enzymatic Activity through Different-sized Gold Nanoparticles

Abstract

We have demonstrated that gold nanoparticles (AuNPs) can effectively associate with enzyme through spontaneous adsorption to afford supramolecular complexes, resulting in a significant increase of its enzymatic activity. In this work, we embarked on a study of the interactions between the different-sized AuNPs and the substrate through quantifying the kinetic parameters (K(cat) and K(m)). The diversity of size allows us to probe the role of catalytic behaviors in modulating enzymatic activity.