標題: | In-channel simplified decoupler with renewable electrochemical detection for microchip capillary electrophoresis |
作者: | Lin, Kuan-Wen Huang, Yan-Kai Su, Hsiu-Li Hsieh, You-Zung 應用化學系 Department of Applied Chemistry |
關鍵字: | microchip capillary electrophoresis;electrochemical detection;decoupler;renewable electrode |
公開日期: | 30-六月-2008 |
摘要: | Electrochemical (EC) detection is comparable to fluorescence detection in that it is simple to perform, economical, and highly sensitive. In this study, we used replica molding to fabricate a PDMS microchip for microchip capillary electrophoresis (CE). A decoupler electrode and a working electrode were implanted into the PDMS chip during the molding process to prevent leakage into the electrode channel. The working electrode could be renewed readily through its slight withdrawal (ca. 3 mm) from the PDMS; its detection ability was highly reproducible in the microchip CE-EC system. The relative standard deviation (R.S.D.) of the detecting current for the renewed working electrode was 1.2% (n = 5). The calibration curves were linear for both dopamine and catechol analytes over the concentration range 10-1000 mu M, with coefficients ofdetermination (R-2) of 0.999 and 0.976, respectively. The number of theoretical plates (N/m) for these analytes was greater than 133,000. (C) 2008 Elsevier B.V. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.aca.2008.02.062 http://hdl.handle.net/11536/29353 |
ISSN: | 0003-2670 |
DOI: | 10.1016/j.aca.2008.02.062 |
期刊: | ANALYTICA CHIMICA ACTA |
Volume: | 619 |
Issue: | 1 |
起始頁: | 115 |
結束頁: | 121 |
顯示於類別: | 會議論文 |