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李耀坤

李耀坤 Li, Yaw-Kuen

電子郵件／E-mail：ykl@faculty.nctu.edu.tw

服務單位／Department：理學院 ／ 應用科技組

著作期間／Publish Period：1994 - 2014-11-01

著作統計／Statistics

Article（51）
Others（5）
Patents（10）
Plan（32）
Proceedings Paper（1）
Thesis（67）

Article

序號
No.		 標題
Title		 著作日期
Date
1 Silicon nanowire field-effect-transistor based biosensors: From sensitive to ultra-sensitive
 2014-10-15
2 Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus
 2014-09-01
3 Rapid construction of an effective antifouling layer on a Au surface via electrodeposition
 2014-01-01
4 Catalytic function of a newly purified exo-beta-D-glucosaminidase from the entomopathogenic fungus Paecilomyces lilacinus
 2013-04-02
5 In situ laser micropatterning of proteins for dynamically arranging living cells
 2013-01-01
6 Preparation of High-Performance Water-Soluble Quantum Dots for Biorecognition through Fluorescence Resonance Energy Transfer
 2012-12-01
7 Water-soluble germanium nanoparticles cause necrotic cell death and the damage can be attenuated by blocking the transduction of necrotic signaling pathway
 2011-12-15
8 Characterization and identification of essential residues of the glycoside hydrolase family 64 laminaripentaose-producing--1, 3-glucanase
 2011-08-01
9 Crystal Structures of Bacillus cereus NCTU2 Chitinase Complexes with Chitooligomers Reveal Novel Substrate Binding for Catalysis A CHITINASE WITHOUT CHITIN BINDING AND INSERTION DOMAINS
 2010-10-08
10 Novel Steroid-Sensing Model and Characterization of Protein Interactions Based on Fluorescence Anisotropy Decay
 2010-04-01
11 Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide
 2009-12-01
12 Structure, Mechanistic Action, and Essential Residues of a GH-64 Enzyme, Laminaripentaose-producing beta-1,3-Glucanase
 2009-09-25
13 Cloning and Expression of Chitinase A from Serratia marcescens for Large-scale Preparation of N,N-Diacetyl Chitobiose 2009-08-01
14 Expression, Purification and Characterization of Human alpha-L-Fucosidase 2009-08-01
15 Detection of an uncharged steroid with a silicon nanowire field-effect transistor
 2009-04-24
16 A straightforward alpha-selective aromatic glycosylation and its application for stereospecific synthesis of 4-methylumbelliferyl alpha-T-antigen
 2009-03-10
17 Identification of Essential Residues of Human alpha-L-Fucosidase and Tests of Its Mechanism
 2009-01-13
18 Structural simulation and protein engineering to convert an endo-chitosanase to an exo-chitosanase
 2008-09-01
19 A possibility of detection of the non-charge based analytes using ultra-thin body field-effect transistors
 2008-07-15
20 Quantitative Determination of Triglyceride by Photoactivated CdSe/ZnS Quantum Dots Through Fluorescence Assay 2008-07-01
21 A new approach for quantitative determination of glucose by using CdSe/ZnS quantum dots
 2008-03-14
22 Fabrication of a miniature CMOS-based optical biosensor
 2007-06-15
23 A highly sensitive system for urea detection by using CdSe/ZnS core-shell quantum dots
 2007-03-15
24 Purification and characterization of a catechol 1,2-dioxygenase from a phenol degrading Candida albicans TL3
 2007-03-01
25 Expression, purification and characterization of a bifunctional alpha-L-arabinofuranosidase/beta-D-xylosidae from Trichoderma koningii G-39 2007-02-01
26 Mutagenesis and mechanistic study of a glycoside hydrolase family 54 alpha-L-arabinofuranosidase from Trichoderma koningii
 2007-01-15
27 Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2
 2006-09-01
28 Discovery of different types of inhibition between the human and Thermotoga maritima alpha-fucosidases by fuconojirimycin-based derivatives
 2006-05-09
29 Design and fabrication of a photosensing nanodevice structure with CdSe and Au nanoparticles on a silicon chip
 2006-05-01
30 Synthesis of activity probes for beta-xylosidase 2006-04-01
31 Facile synthesis toward the construction of an activity probe library for glycosidases
 2006-03-20
32 Exploration of glycosyl hydrolase family 75, a chitosanase from Aspergillus fumigatus 2006-02-10
33 An isolated Candida albicans TL3 capable of degrading phenol at large concentration
 2005-12-01
34 A chitosanase from Paecilomyces lilacinus with binding affinity for specific chito-oligosaccharides 2005-04-01
35 Detection of serum uric acid using the optical polymeric enzyme biochip system
 2004-07-15
36 CMOS chip as luminescent sensor for biochemical reactions
 2003-06-01
37 Aqueous two-phase extraction as an effective tool for isolation of geniposide from gardenia fruit
 2002-11-22
38 Design and synthesis of activity probes for glycosidases
 2002-10-17
39 Identification of the two essential groups in the family 3 beta-glucosidase from Flavobacterium meningosepticum by labelling and tandem mass spectrometric analysis
 2002-08-01
40 Purification, characterization and cloning of a chitinase from Bacillus sp NCTU2 2002-06-01
41 Identification of the general acid/base catalyst of a family 3 beta-glucosidase from Flavobacterium meningosepticum
 2002-02-26
42 Catalytic mechanism of a family 3 beta-glucosidase and mutagenesis study on residue Asp-247
 2001-05-01
43 Rapid process for purification of an extracellular beta-xylosidase by aqueous two-phase extraction
 2001-04-15
44 Effective extraction and purification of beta-xylosidase from Trichoderma koningii fermentation culture by aqueous two-phase partitioning
 2001-02-01
45 An Aspergillus chitosanase with potential for large-scale preparation of chitosan oligosaccharides 2000-12-01
46 Effective induction, purification and characterization of Trichoderma koningii G-39 beta-xylosidase with high transferase activity 2000-04-01
47 Mechanistic study of beta-xylosidase from Trichoderma koningii G-39
 2000-02-01
48 Cloning and expression of beta-glucosidase from Flavobacterium meningosepticum: A new member of family B beta-glucosidase
 1999-02-01
49 Purification, characterization and mechanistic study of beta-glucosidase from Flavobacterium meningosepticum (ATCC 13253) 1998-10-01
50 New imidazoles as probes of the active site topology and potent inhibitors of beta-glucosidase
 1998-03-01
51 Characterization of an isozyme of beta-glucosidase from sweet almond 1997-02-01

Others

序號
No.		 標題
Title		 著作日期
Date
1 Mechanistic and structural study of BcGT 2013-07-01
2 "A marcrolide glucosyltransferase promoting the synthesis of O-, S- and N-glucosidic compounds" 2012-09-01
3 Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide
 2009-11-01
4 Mutagenesis and kinetic study of human alpha-l-fucosidase
 2005-07-01
5 A new imidazoles as potent inhibitors of beta-glucosidase. 1996-08-25

Patents

序號
No.		 標題
Title		 著作日期
Date
1 具硼酸基團連接子及含有其之生物感測元件
 2014-11-01
2 磁性奈米複合材料及其製造方法及應用
 2014-10-11
3 純化蛋白質的方法
 2014-02-01
4 具硼酸基團連接子及含有其之生物感測元件
 2014-01-01
5 磁性奈米複合材料及其製造方法及應用
 2013-09-16
6 感測元件、製造方法及其生物檢測系統
 2013-01-21
7 利用酵素法合成醣基化合物
 2012-11-16
8 用於純化蛋白質之載體、方法、系統與套組
 2011-07-01
9 感測元件、製造方法及其生物檢測系統
 2010-04-01
10 SENSING ELEMENT, MANUFACTURING METHOD THEREOF, AND BIOLOGICAL DETECTION SYSTEM EMPLOYING SUCH SENSING ELEMENT
 2010-03-25

Plan

序號
No.		 標題
Title		 著作日期
Date
1 設計並建立以酵素催化合成醣基化黃酮素化合物之平台 2014
2 化學學門(有機組與無機組)研究發展及推動計畫 2013
3 設計並建立以酵素催化合成醣基化黃酮素化合物之平台 2013
4 化學學門(有機組與無機組)研究發展及推動計畫 2012
5 設計並建立以酵素催化合成醣基化黃酮素化合物之平台 2012
6 研究能自發斷裂之胜？並應用於提高蛋白質純化之效能 2011
7 化學學門(有機組與無機組)研究發展及推動計畫 2011
8 研究能自發斷裂之胜太並應用於提高蛋白質純化之效能 2010
9 建立醣甘水解酵素之生物資訊分析平台---以LPHase, MTSase,及MTHase為應用模型--子計畫三:結合生物資訊與結構模擬研究第64家族beta-glucanase之活性區與重要胺基酸殘基(III)
 2010
10 人類透明質酸水解脢和其抑制劑之研究開發 2009
11 建立醣甘水解酵素之生物資訊分析平台---以LPHase,MTSase,及MTHase為應用模型---(子計畫三)結合生物資訊與結構模擬研究第64家族beta-glucanase之活性區與重要胺基酸殘基(II) 2009
12 人類透明質酸水解脢和其抑制劑之研究開發 2008
13 建立醣甘水解酵素之生物資訊分析平台---以LPHase, MTSase及MTHase為應用模型---(子計畫三):結合生物資訊與結構模擬研究第64家族Beta-Glucanase之活性區與重要胺基酸殘基(I) 2008
14 人類透明質酸水解脢和其抑制劑之研究開發 2007
15 發展多功能奈米粒子系統進行專一性細胞胞器偵測及細胞毒殺操作---計畫三：具位向專一之蛋白質修飾及其在奈米生醫之運用(II) 2007
16 人類 alpha-L-岩藻醣甘酵素之結構與催化機構的研究(II) 2006
17 發展多功能奈米粒子系統進行專一性細胞胞器偵測及細胞毒殺操作---子計畫三：具位向專一之蛋白質修飾及其在奈米生醫之運用(I) 2006
18 人類 Alpha-L-岩藻醣甘酵素之結構與催化機構的研究(I)
 2005
19 特殊Beta-1,3-葡萄五醣生產脢之反應機構研究與其應用之開發(III) 2005
20 特殊Beta-1,3-葡萄五醣生產脢之反應機構研究與其應用之開發(II)
 2004
21 挑戰聚醣胺水解酵素之自然設計(III)
 2004
22 特殊Beta-1,3-葡萄五醣生產脢之反應機構研究與其應用之開發(I) 2003
23 挑戰聚醣胺水解酵素之自然設計(II) 2003
24 挑戰聚醣胺水解酵素之自然設計(I)
 2002
25 幾丁質類物質在食品、農業、化工及醫藥上之應用---以酵素法製備單一成份之寡醣胺
 2001
26 以酵素基因工程重建幾丁質酵素之產物特異性 (II)基因之過表達與以突變法進行酵素功能之改質
 2001
27 以酵素基因工程重建幾丁質酵素之產物特異性---基因選植與分解酵素之催化研究(I)
 2000
28 以定向演化研究β-葡萄糖甘酵素之活性區及其非自然演化之β-糖甘類酵素
 2000
29 以動力學和基因定點突變研究黃質菌β-葡萄糖酵素之催化機構
 1999
30 醣類水解酵素之性質研究與應用之開發 1997
31 醣類水解酵素之性質研究與應用之開發 1996
32 β-葡萄醣甘酵素之催化反應機構與應用之研究 1995

Proceedings Paper

序號
No.		 標題
Title		 著作日期
Date
1 Laser-Assisted Control of Protein Adsorption for Dynamically Arranging Viable Cells 2013-01-01

Thesis

序號
No.		 標題
Title		 著作日期
Date
1 建構以磁性粒子為基礎之修飾方式及其在蛋白質純化之效率探討 2014
2 生物分子與介面化學: 從有機合成到生醫應用 2014
3 豌豆蔗糖合成酶的表現、性質研究和應用 2013
4 多重苯硼酸衍生物之設計、合成和生物感測之應用 2013
5 發展對B肝病毒表面抗原有專一性之單株抗體 2013
6 枯草桿菌醣基轉移酶BcGT-1中Phe240、Phe132及Phe138胺基酸殘基對類黃酮醣基化之影響 2013
7 利用融合蛋白技術製備動物生長激素及N端切醣酵素 2013
8 篩選具辨識環單磷酸腺苷之抗體片段並探討其結合專一性 2013
9 以蛋白質突變技術增強Sortase A之轉肽活性 2013
10 Diels-Alder反應之外向加成 2013
11 Paecilomyces Lilacinus 中外切型葡萄糖胺苷水解酵素之研究暨生醫感測技術之開發 2012
12 探討Sortase A突變株D165A、K196T和P94S/D160N/D165A/K196T的反應性
 2012
13 利用苯硼酸衍生物作為晶片表面修飾並應用於人體血清中登革熱病毒檢測 2012
14 枯草桿菌UDP-glucosyltransferase中Phe240胺基酸殘基對檞黃素醣基化之影響 2012
15 醣苷水解酵素家族的反應機制與重要催化殘基之鑑定 2010
16 枯草桿菌UDP-glycosyltransferase之受質專一性及應用研究 2010
17 嵌在人類長分散子1（LINE-1）中的簡單重覆序列的演化 2010
18 枯草桿菌UDP-glycosyltransferase之重要胺基酸殘基分析及其應用 2010
19 利用苯硼酸衍生物作為抗體專一位向固定化並應用於前降鈣素檢測 2010
20 利用磁性奈米複合材料作為樣品前處理及蛋白質消化以應用於質譜分析 2010
21 磁性奈米複合材料應用於高效能蛋白質純化 2010
22 枯草桿菌UDP-glycosyltransferase之受質專一性研究與快速活性偵測法建立 2010
23 煙麴菌α-半乳糖苷酶的過量表現並利用基因工程改質為糖苷鍵合成酶 2009
24 以基因突變技術轉換地衣聚醣酶成為醣類合成□ 2009
25 利用奈米金粒子建立快篩玻尿酸水解酶抑制劑之平台 2009
26 利用大腸桿菌胞外分泌蛋白質融合表現人類玻尿酸合成酶 2009
27 透過結構模擬與蛋白質工程重組幾丁聚醣水解酵素之研究
 2008
28 醣苷水解酵素家族29與64的反應機制與重要催化殘基之鑑定
 2008
29 以場效電晶體為平台,建立一套新穎的偵測方法應用於中性小分子分析物之檢測
 2008
30 含奈米粒子之先進奈米元件結構應用於太陽能電池之研究
 2008
31 幾丁質酵素和幾丁質結合蛋白之基因選殖、蛋白質結構和反應機制以及其應用之研究
 2008
32 幾丁質結合蛋白的應用 2007
33 高效能水溶性量子點之製備與其在生物檢測之應用
 2007
34 先進奈米元件結構於光感測與太陽能電池之研究
 2007
35 可降解酚之Candida albicans TL3及其catechol 1,2-dioxygenase之單離與特性探討
 2006
36 探討α-L-arabinofuranosidase E223單點突變造成的反應機制之改變
 2006
37 Trichoderma koningii G-39中雙功能阿拉伯呋喃糖苷酵素/木糖苷酵素的過量表現、突變以及反應機制的研究
 2005
38 家族54阿拉伯呋喃糖苷酵素之反應機制探討
 2005
39 以硒化鎘/硫化鋅與金奈米粒子建構先進之光感測元件
 2005
40 人類岩藻醣苷水解酶之抑制研究
 2005
41 矽晶片上之硒化鎘與金奈米粒子感光元件設計與製程
 2004
42 建立與評估具胞外分泌特性之TA表現載體 2004
43 突變研究與探討煙麴菌幾丁聚醣酵素之催化必要殘基
 2004
44 仙人掌桿菌幾丁質酵素的過量表現與催化反應機制的探討
 2003
45 血糖生化檢測之研究 2002
46 基因改造黏質沙雷氏桿菌幾丁質酵素之特性 2002
47 以基因重組人類二氫葉酸還原酶建立抗癌藥之篩選系統 2002
48 微生物幾丁聚醣酵素之探討 2002
49 以巨型桿菌及大腸桿菌系統表現幾丁聚醣酵素及其化學修飾後的催化性質之研究 2002
50 Paecilomyces lilacinus中幾丁聚醣酶與葡萄糖胺苷水解酶之研究 2002
51 木腐黴菌之阿拉伯呋喃糖苷酶的過量表現與催化功能研究 2002
52 Flavobacterium meningosepticum中第三家族β-葡萄糖甘酵素的反應機制研究及其親核性基團與一般酸鹼催化基團之鑑定 2001
53 △5-3-酮基類固醇異構酵素突變株化學修飾之研究 2001
54 仙人掌桿菌中幾丁質酵素的選殖與表現之研究 2001
55 利用雙水相萃取純化分離生物分子及木糖□水解酵素的催化機構與應用研究 2000
56 仙人掌桿菌NCTU4幾丁聚醣酵素之性質研究與應用 2000
57 黃質菌中 Catalase-Peroxidase 之純化與性質研究 1998
58 木腐菌β-木糖甘酵素反應機制研究 1998
59 放射菌NCTU3中幾丁質酵素之篩選、純化及性質的研究 1998
60 Flavobacterium meningosepticum之β-葡萄糖甘酵素的反應機制研究 1997
61 葡萄糖甘酵素的基因選殖與純化 1996
62 Trichoderma koningii G-39之β-木糖甘酵素之純化與性質研究 1995
63 Purification and Characterization of β-Xyiosidase from Trichoderma koningii G-39 1995
64 Applications of β-Glucosidase in Organic Synthesis 1995
65 Purification and Characterization of β-Glucosidase from Flavobacterium meningosepticum 1995
66 甜杏仁葡萄糖甘酵素之受質專一性與其抑制作用的研究 1994
67 甜杏仁之葡萄甘酵素的特性與抑制作用之研究 1994
理學院