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吳東昆

吳東昆 Wu, Tung-Kung

電子郵件／E-mail：tkwmll@mail.nctu.edu.tw

服務單位／Department：生物科技學院 ／ 生物科技學系

著作期間／Publish Period：1993-08-01 - 2014

著作統計／Statistics

Article（48）
Books（1）
Others（23）
Patents（1）
Plan（29）
Proceedings Paper（2）
Thesis（58）

Article

序號
No.		 標題
Title		 著作日期
Date
1 Ligand-Functionalization of BPEI-Coated YVO4:Bi3+,Eu3+ Nanophosphors for Tumor-Cell-Targeted Imaging Applications
 2013-11-01
2 Immunocapture couples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid detection of type 1 dengue virus
 2013-05-03
3 Reconstitution of the early steps of gliotoxin biosynthesis in Aspergillus nidulans reveals the role of the monooxygenase GliC
 2013-04-01
4 The Thermostable Direct Hemolysin from Grimontia hollisae Causes Acute Hepatotoxicity In Vitro and In Vivo
 2013-02-21
5 Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping
 2013-02-15
6 Protein engineering of oxidosqualene-lanosterol cyclase into triterpene monocyclase
 2013-01-01
7 Molecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesis
 2012-11-01
8 The cysteine 703 to isoleucine or histidine mutation of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae generates an iridal-type triterpenoid
 2012-11-01
9 Protein Engineering of Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase into Parkeol Synthase
 2012-10-19
10 Predictive factors of the duration of a first-attack acute urticaria in children
 2011-10-01
11 Purification and Properties of a Novel Phenolic Antioxidant from Radix astragali Fermented by Aspergillus oryzae M29
 2011-06-22
12 Poison exposure and outcome of children admitted to a pediatric emergency department
 2011-05-01
13 Gold nanoparticle-based inductively coupled plasma mass spectrometry amplification and magnetic separation for the sensitive detection of a virus-specific RNA sequence
 2011-04-08
14 Crystal Structure and Inhibition Studies of Transglutaminase from Streptomyces mobaraense
 2011-03-04
15 Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae
 2011-02-01
16 Photoactivation Studies of Zinc Porphyrin-Myoglobin System and Its Application for Light-Chemical Energy Conversion
 2011-01-01
17 Site-Directed Mutations of Thermostable Direct Hemolysin from Grimontia hollisae Alter Its Arrhenius Effect and Biophysical Properties
 2011-01-01
18 Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterol's C/D-ring cyclization and 17 alpha/beta-exocyclic side chain stereochemistry
 2011-01-01
19 Crystal Structure and Mutational Analysis of Aminoacylhistidine Dipeptidase from Vibrio alginolyticus Reveal a New Architecture of M20 Metallopeptidases
 2010-12-01
20 Significant factors associated with severity and outcome of an initial episode of acute urticaria in children
 2010-11-01
21 Post-resuscitative clinical features in the first hour after achieving sustained ROSC predict the duration of survival in children with non-traumatic out-of-hospital cardiac arrest
 2010-04-01
22 Factors associated with sustained return of spontaneous circulation in children after out-of-hospital cardiac arrest of noncardiac origin
 2010-03-01
23 Alteration of the Substrate's Prefolded Conformation and Cyclization Stereochemistry of Oxidosqualene-Lanosterol Cyclase of Saccharomyces cerevisiae by Substitution at Phenylalanine 699
 2010-02-05
24 Anticancer and Antioxidant Activities of the Peptide Fraction from Algae Protein Waste
 2010-01-27
25 Differential Stereocontrolled Formation of Tricyclic Triterpenes by Mutation of Tyrosine 99 of the Oxidosqualene-Lanosterol Cyclase from Saccharomyces cerevisiae
 2009-11-01
26 Isolation and characterisation of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from the algae protein waste
 2009-07-01
27 Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems
 2009-07-01
28 Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis
 2009-04-01
29 Purification, crystallization and preliminary X-ray analysis of an aminoacylhistidine dipeptidase (PepD) from Vibrio alginolyticus
 2009-03-01
30 Importance of Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase Tyrosine 707 Residue for Chair-Boat Bicyclic Ring Formation and Deprotonation Reactions
 2008-11-06
31 Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus
 2008-10-01
32 Protein plasticity: A single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien-3 beta-ol, a rearrangement product
 2008-06-19
33 Identification of a Vibtio furnissii oligopeptide permease and characterization of its in vitro hemolytic activity
 2007-11-01
34 Ultrafast relaxation of zinc protoporphyrin encapsulated within apomyoglobin in buffer solutions
 2007-07-05
35 Fabrication of a miniature CMOS-based optical biosensor
 2007-06-15
36 Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression
 2007-06-13
37 The effect of centaurein on interferon-gamma expression and Listeria infection in mice
 2007-02-15
38 Phenylalanine 445 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences C-ring cyclization and deprotonation reactions
 2006-10-12
39 Site-saturated mutagenesis of histidine 234 of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase demonstrates dual functions in cyclization and rearrangement reactions
 2006-05-17
40 Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions
 2006-03-30
41 Histidine residue at position 234 of oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae simultaneously influences cyclization, rearrangement, and deprotonation reactions
 2005-07-01
42 Enzymatic formation of multiple triterpenes by mutation of tyrosine 510 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae
 2004-12-03
43 Polyacetylenic compounds and butanol fraction from Bidens pilosa can modulate the differentiation of helper T cells and prevent autoimmune diabetes in non-obese diabetic mice 2004-11-01
44 Detection of serum uric acid using the optical polymeric enzyme biochip system
 2004-07-15
45 Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver
 2004-01-01
46 CMOS chip as luminescent sensor for biochemical reactions
 2003-06-01
47 Conversion of a plant oxidosqualene-cycloartenol synthase to an oxidosqualene-lanosterol cyclase by random mutagenesis
 2002-07-02
48 OPTIMUM DESIGN OF LIQUID-CRYSTAL DISPLAY PARAMETERS IN PROJECTION AND DIRECT VIEW APPLICATIONS
 1993-08-01

Books

序號
No.		 標題
Title		 著作日期
Date
1 國立交通大學生物科技學系吳東昆教師升等送審著作論文集 2008

Others

序號
No.		 標題
Title		 著作日期
Date
1 Crystal structure and mutational analysis of thermostable direct hemolysin from Grimontia hollisae reveals new insights on membrane binding and physiological activity 2013-07-01
2 Functional expression of the triterpenoid biosynthesis tailoring enzyme on enzymatic modification of sterol derivatives
 2012-09-01
3 "Mutated Thermostable Direct Hemolysin from Grimontia hollisae Affects Its Hemolytic Activity, Membrane Binding, and Protein Quaternary Structure" 2012-08-01
4 Photoactivation studies of zinc protoporphyrin-myoglobin system and its application for light-chemical energy conversion 2011-08-28
5 Biochemical and physiochemical characterization of thermostable ditrect hemolysin from grimontia hollisae 2010-06-01
6 Photo-induced chemical reduction of NADP plus by reconstituted metalloporphyrin myoglobin: An artificial photosynthesis model for photosystem I 2009-08-16
7 Altering oxidosqualene cyclization from chair-boat-chair to chair-chair-chair biosynthetic route by a single amino acid substitution at the phenylalanine 699 position of the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase 2009-08-16
8 Preliminary structure determination and functional residues characterization of thermostable direct hemolysin from Grimontia hollisae 2009-08-16
9 Thermostable direct hemolysin from Grimontia hollisae exhibits Arrhenius effect and in vitro cytotoxicity 2009-08-16
10 Tryptophan 587 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences substrate binding 2009-08-16
11 Crystal structure of aminoacylhistidine dipeptidase from vibrio alginolyticus, a metallopeptidase with dinuclear metal center. 2009-08-16
12 Crystal structure of aminoacylpeptidase dipeptidase from Vibrio alginolyticus, a metallopeptidase with dinuclear center 2009-07-01
13 Structural and functional characterization of aminoacylhistidine dipeptidase from Vibrio alginolyticus, a metallopeptidase with dinuclear metal center
 2008-06-01
14 Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase: A Chemistry-Biology Interdisciplinary Study of the Protein's Structure-Function-Reaction Mechanism Relationships
 2008-01-01
15 ORGN 611-Molecular cloning and inhibition studies of oxidosqualene-lanosterol cyclase from bovine liver using a novel Ro48-8071 based fluorescence probe 2006-09-10
16 BIOT 373-Artificial myoglobin system design for electron transfer mechanism study and BioSolar cell applications 2006-09-10
17 ORGN 610-Identification of a putative active site residue within oxidosqualene-lanosterol cyclase from saccharomyces cerevisiae that influences B/C ring cyclization 2006-09-10
18 ORGN 608-Enzymatic formation of multiple triterpenes by mutation of histidine 234 of the oxidosqualene-lanosterol cyclase from saccharomyces cerevisiae 2006-09-10
19 ORGN 609-Site-saturated mutagenesis of phenylalanine 445 of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase results in production of isomalabaricane triterpenoid and parkeol 2006-09-10
20 Characterization of a bi-functional protein with hemolysin and ABC transporter activity 2006-09-10
21 Molecular cloning and functional characterization of hemolysin gene from Vibrio furnissii
 2005-07-01
22 Enzymatic formation of multiple triterpenes by mutation of tyrosine 510 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae. 2005-03-13
23 Functional analysis of alanine-scanning and site-directed mutations in the oxidosqualene-lanosterol cyclase gene of Saccharomyces cerevisiae 2001-03-07

Patents

序號
No.		 標題
Title		 著作日期
Date
1 快速篩檢變異血紅素之方法
 2012-03-16

Plan

序號
No.		 標題
Title		 著作日期
Date
1 三帖皂?生合成酵素之蛋白質工程及其產生具生物活性化合物之研究 2014
2 以臺灣鱟醣分子結合蛋白抑制病原性細菌-利用單株抗體結合質譜技術探討 HPL-多醣作用之醣蛋白體特徵(子計畫二)( I ) 2014
3 三帖皂?生合成酵素之蛋白質工程及其產生具生物活性化合物之研究 2013
4 氧化鯊烯環化酵素之易曲性及其於形成具生物活性產物之研究 2012
5 結核分枝桿菌醣基轉移酵素之基因選殖與光親和性標記對酵素活性抑制研究 2012
6 胺醯組氨酸雙胜？？？活性區域之蛋白質工程研究 2011
7 氧化鯊烯環化酵素之易曲性及其於形成具生物活性產物之研究 2011
8 氧化鯊烯環化酵素之易曲性及其於形成具生物活性產物之研究 2010
9 胺醯組氨酸雙胜太活性區域之蛋白質工程研究 2010
10 仿生太陽能電池元件及其光電能量轉換機制研究---總計畫(III) 2009
11 仿生太陽能電池元件及其光電能量轉換機制研究---子計畫一(III)
 2009
12 仿生太陽能電池元件及其光電能量轉換機制研究（創新啟動補助方案） 2009
13 仿生太陽能電池元件及其光電能量轉換機制研究---子計畫一：(II) 2008
14 仿生太陽能電池元件及其光電能量轉換機制研究---總計畫(II) 2008
15 仿生太陽能電池元件及其光電能量轉換機制研究---子計畫一(I) 2007
16 仿生太陽能電池元件及其光電能量轉換機制研究---總計畫(I) 2007
17 白斑病毒胸腺嘧啶激脢作為抗病毒藥物篩選之研究 2006
18 利用氧化鯊烯環化酵素作為篩選胜月太適合體及其擬胜月太之研究(II) 2006
19 利用氧化鯊烯環化酵素作為篩選胜﹝月太﹞適合體及其擬胜﹝月太﹞之研究(I)
 2005
20 利用氧化鯊烯環化酵素作為篩選胜太/核酸Aptamer之研究
 2004
21 氧化鯊烯環化酵素之蛋白質-核酸交互作用研究 2003
22 白斑病毒胸腺嘧啶激脢之蛋白質工程與酵素活性關係之研究 2003
23 氧化鯊烯環化酵素之產物多樣性與開發寡核酸先導藥物之研究
 2002
24 白斑病毒之胸腺嘧啶激脢作為病毒抑制劑標的之研究 2002
25 弧菌屬微生物代謝產物之分析、活性測試及其固定化方法之研發與改進 (I) 2001
26 氧化鯊烯環化酵素之作用機制探討與酵素結構鑑定(II) 2001
27 氧化鯊烯環化酵素之作用機制探討與酵素結構鑑定(I) 2000
28 氧化鯊烯環化酵素之導向演化的研究及其光親和標記的設計與合成
 2000
29 氧化鯊烯環化酵素之結構與反應特性之研究及其抑制劑之設計與合成
 1999

Proceedings Paper

序號
No.		 標題
Title		 著作日期
Date
1 AC electrokinetics assisted impedance biosensors for rapid bacteria detection 2012-01-01
2 Fabrication and Photovoltaic Characterization of Bio-Sensitized Solar Cells Using Myoglobin-Based Sensitizers 2009-03-01

Thesis

序號
No.		 標題
Title		 著作日期
Date
1 發展對B肝病毒表面抗原有專一性之單株抗體 2013
2 以鐵鐵氫化酶為基礎仿生合成金屬氫簇族並進行光催化產氫研究 2013
3 合成仿生性釕磷基氫團簇並應用於光催化產氫 2013
4 合成與應用光敏物-鍵橋-催化團簇(P-L-C)模型於光催化產氫系統 2013
5 麴菌中二次代謝物生合成產物之鑑定與解析研究 2012
6 霍氏格里蒙菌熱穩定性溶血素造成活體內外肝臟毒性的探討
 2012
7 Functional Characterization of Thermostable Direct Hemolysin from Grimontia Hollisae and 2012
8 仿生性釕基氫團簇之合成及其光催化產氫之應用研究 2012
9 登革熱病毒專一性單株抗體之開發及應用 2012
10 蒺藜苜蓿、阿拉伯芥及抗菌素鏈黴菌中葡萄糖轉醣酶之分子選殖、蛋白質表現及其功能性分析 2012
11 發展針對胃癌標定分子具專一性的單株抗體 2012
12 人工重組鋅紫質-肌紅蛋白系統應用於光催化能量轉換之研究 2011
13 利用重組鋅-乙炔苯酸紫質與螢光團共價修飾之肌紅蛋白突變株作為雙核的光催化系統進行其光能轉化學能之研究 2011
14 甘草甜素及阿拉伯芥中細胞色素P450 氫氧化酶之分子選殖、蛋白質表現及其功能性分析 2011
15 探討突變效應影響霍氏格里蒙菌之熱穩定性溶血素的溶血活性、膜結合能力及其蛋白質四級結構之研究 2011
16 開發對原發性肝癌具有專一性之抗體及其應用
 2011
17 Synthesis of Biomimetic Dithiolate-Bridged Osmium-Sulfur Clusters and Their Applications for Photocatalytic Hydrogen Production 2011
18 溶藻弧菌胺醯組胺酸雙胜肽酶之晶體結構與突變分析
 2010
19 利用定點飽和突變技術對於酵母菌氧化鯊烯環化酵素中Gly383及Thr384進行功能性分析並藉由表現之蒺藜苜蓿轉醣酵素對三萜類化合物進行醣基衍生化之研究
 2010
20 仿生性雙硫醇架橋-雙核釕複合物及其衍生物模擬雙硫鐵產氫酶活化中心之研究 2010
21 利用飽和定點突變方法探討酵母菌氧化鯊烯環化酵素其胺基酸Val454與Asn700的功能並搭配表現之阿拉伯芥轉醣酵素進行固醇化合物轉醣衍生化的研究 2010
22 利用突變策略針對氧化鯊烯環化酵素進行其結構與功能及其產物專一性/多樣性之研究 2009
23 利用定點飽和突變的方式對於氧化鯊烯環化酵素內 假設活性區域殘基進行結構與反應關係之研究
 2009
24 利用重組鋅-原紫質及鋅-乙炔苯酸紫質之不同肌紅蛋白突變株進行其光能轉化學能的研究
 2009
25 利用定點突變研究不同物種之氧化鯊烯環化酵素假設活性區內氨基酸之角色
 2009
26 探討霍氏格里蒙菌中熱穩定性溶血素之原始型與突變型生化活性與生物物理特性的差異
 2009
27 利用定點突變進行氧化鯊烯環化酵素家族假設活性區中胺基酸之特性探討
 2009
28 鑑定與解析咸豐草中的免疫調節物質
 2008
29 Photo-Induced Electron Transfer Study of Free-base and Metallo Carboxyphenylethynyl Porphyrins Based Artificial Myoglobin Systems and Their Biosolar Cell Application
 2008
30 利用飽和定點突變方法針對酵母菌氧化鯊烯環化酵素內 Ile705 和豌豆氧化鯊烯-β-麥胚固醇環化酵素假設活性區內的 Leu734 兩者的結構影響在環化及重組過程中的功能性分析
 2008
31 Leu29與His93位置之飽和定點突變抹香鯨肌紅蛋白對其過氧化能力之影響
 2008
32 溶血素及非溶血素基因之溶血活性鑑定及生化特性研究 2008
33 利用定點飽和突變的方法針對酵母菌中氧化鯊烯環化酵素內高度保留之芳香族胺基酸進行功能性的分析
 2007
34 利用飽和定點突變方法探討酵母菌氧化鯊烯環化酵素內Tyr707 和Tyr710 對反應過程中B 環形成的影響
 2007
35 溶藻弧菌胺醯組胺酸雙胜肽酶功能性胺基酸於催化特性分析之研究
 2007
36 利用突變策略、抑制作用與SELEX技術針對氧化鯊烯環化酵素進行功能性之探討
 2007
37 利用定點飽和突變研究抹香鯨肌紅蛋白中Ile-107位置對其過氧化能力之影響
 2006
38 台灣粗榧樹葉中具生物活性成分之分離與鑑定
 2006
39 溶藻弧菌胺醯組胺酸雙胜肽酶之序列與生化特性分析及其功能性胺基酸之研究
 2006
40 利用飽和定點突變方法研究酵母菌氧化鯊烯環化酵素內的假設活性區胺基酸對於催化環化/重組反應的影響
 2006
41 利用飽和定點突變針對氧化鯊烯環化酵素內假設活性區域胺基酸進行結構─反應之研究
 2006
42 溶藻弧菌胺醯組胺酸雙胜肽酶之生化特性分析及其功能性胺基酸之研究
 2006
43 發展及應用以Ro48-8071為基礎的新型式螢光性探針並針對氧化鯊烯環化酵素進行抑制作用的研究
 2006
44 使用重組蛋白製造對 HPV、IHHNV 和TSV殼蛋白有專一性之單株抗體
 2005
45 利用噬菌體胜肽庫篩選對牛肝氧化鯊烯酵素有專一性的胜肽序列
 2004
46 利用定點突變對氧化鯊烯環化酵素的假設活性區進行功能性分析
 2004
47 I. 與哈威弧菌結合之多胜肽適合體之篩選 II. 河流弧菌中推定的溶血素之純化
 2004
48 利用生物資訊方法探討白點症病毒之胸腺嘧啶激酶－胸腺嘧啶磷酸激酶
 2003
49 尿酸氧化酵素之分子導向演化及其於生物晶片系統之應用
 2003
50 利用指數放大的系統性配位基演化技術篩選辨識clavulanic acid生合成途徑中轉錄因子ClaR之DNA序列 2002
51 牛肝中氧化鯊烯-羊毛硬脂醇環化酵素之生化特性與抑制作用的探討 2002
52 分析經雙點突變後之阿拉伯芥中氧化鲨烯-環阿屯醇環化酵素的功能與機制 2002
53 自組裝單層膜技術在蛋白質生物晶片的應用：利用烷基硫醇化合物將山葵過氧化酵素固定在晶片的金表層 2002
54 哈威弧菌中致病因子溶血素之分子選殖與表現 2001
55 酵母菌中氧化鯊烯-羊毛硬脂醇環化酵素的突變效應研究 2000
56 牛肝中氧化鯊烯-羊毛硬脂醇環化酵素的純化和其生化特性上的探討 2000
57 阿拉伯芥中氧化鯊烯-環阿屯醇環化酵素的突變效應研究 2000
58 利用定點突變研究酵母菌中氧化鯊烯-羊毛硬脂醇環化酵素與反應機制間的關係 1999
生物科技學院