Browsing by Issue Date, starting with "2011-04-08"

Now showing 1 - 20 of 41675

Gold nanoparticle-based inductively coupled plasma mass spectrometry amplification and magnetic separation for the sensitive detection of a virus-specific RNA sequence
(2011-04-08)
Hsu, I-Hsiang
;
Chen, Wen-Hung
;
Wu, Tung-Kung
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Sun, Yuh-Chang
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生物科技學系
We have developed a sensitive gold nanoparticle (AuNP)-based inductively coupled plasma mass spectrometry (ICP-MS) amplification and magnetic separation method for the detection of oligonucleotide sequences. The assay relies on (i) the sandwich-type binding of two designed probe sequences that specifically recognize the target oligonucleotide sequences, (ii) magnetic bead separation, and (iii) AuNP-based ICP-MS amplification detection. To enhance the analytical signal and minimize the background signal resulting from nonspecific binding, we performed a series of experiments to evaluate the effects of various parameters (the concentration of the capture probe; the time required for hybridization; the number of washings required to eliminate nonspecific binding) on the oligonucleotide detection. Under the optimized conditions, the detection limit was 80 zmol (corresponding to 1.6fM of the target sequence in a sample volume of 50 mu L). Moreover, it employs a shorter hybridization step and ICP-MS, this procedure is relatively simple and rapid (ca. 1.5 h). Based on the analytical results obtained using complementary and mismatched sequences, our method exhibits good performance in distinguishing complementary and random oligonucleotides. Compared with the "gold standard" methodology (plaque assay) for the quantification of dengue virus, our method has the capability to allow early detection of dengue virus in complicated and small-volume samples, with high specificity, good analytical sensitivity, and superior time-effectiveness. (C) 2011 Elsevier B.V. All rights reserved.
Free lunches on the discrete Lipschitz class
(2011-04-08)
Jiang, Pei
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Chen, Ying-ping
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資訊工程學系
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Department of Computer Science
The No-Free-Lunch theorem states that there does not exist a genuine general-purpose optimizer because all algorithms have the identical performance on average over all functions. However, such a result does not imply that search heuristics or optimization algorithms are futile if we are more cautious with the applicability of these methods and the search space. In this paper, within the No-Free-Lunch framework, we firstly introduce the discrete Lipschitz class by transferring the Lipschitz functions, i.e., functions with bounded slope, as a measure to fulfill the notion of continuity in discrete functions. We then investigate the properties of the discrete Lipschitz class, generalize an algorithm called subthreshold-seeker for optimization, and show that the generalized subthreshold-seeker outperforms random search on this class. Finally, we propose a tractable sampling-test scheme to empirically demonstrate the superiority of the generalized subthreshold-seeker under practical configurations. This study concludes that there exist algorithms outperforming random search on the discrete Lipschitz class in both theoretical and practical aspects and indicates that the effectiveness of search heuristics may not be universal but still general in some broad sense. (C) 2010 Elsevier B.V. All rights reserved.
幾句夢話
(2011-04-10)
顏學廷
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Department of Communication and Technology
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傳播與科技學系
揉合著怪誕世界的綺麗景象、和朋友討論原文雙關諺語的聲音、各式遊戲的精彩冒險、還有圖書館的氣味，就成了不同於球場或街頭的年少回憶。
行銷城市，悲不悲情?
(2011-04-10)
鄭家宜
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Department of Communication and Technology
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傳播與科技學系
在黃泥土地上綠意盎然的山頭，在平房裡的純樸真性情居民，這些原始模樣只停留在《悲情城市》的記憶中。世界看到了九份，九份卻失去了自己。
展現活力歌仔戲新風情
(2011-04-10)
李庭蕙
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Department of Communication and Technology
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傳播與科技學系
歌仔戲是個充滿活力的娛樂文化，不過許多人對歌仔戲持有負面的刻板印象，例如老派八股或是過於悲情。的確，在過去歌仔戲的表演，對觀眾而言是種情感的宣洩與抒發，因此會有這種刻板印象。但是 ...
「橋」出柒仟壹佰陸拾柒萬
(2011-04-10)
王升含
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Department of Communication and Technology
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傳播與科技學系
學生正焦頭爛額地面對期末考攻勢，上班族偷偷開始計畫暑假旅遊行程，卻有一群頭戴安全帽、皮膚黝黑的人們，戰戰兢兢地展開南投縣國姓橋的拓寬工程。
赤裸誠實　讓悲傷坦然共存　
(2011-04-10)
羅文婕
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Department of Communication and Technology
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傳播與科技學系
五味雜陳的生命史，除了感性中見唯美外，更動人的，是作者對自身，赤裸誠實的書寫態度。
尋根客家
(2011-04-10)
李宜融
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Department of Communication and Technology
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傳播與科技學系
於是我倚著一片舊有的過去，歡愉地迎向所有未知的可能性。
塗鴉破壞　摸乳巷中的非分者　
(2011-04-10)
陳羽涵
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Department of Communication and Technology
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傳播與科技學系
行走在鹿港的街道上，會發現到許多狹窄巷道，大部分是鄉里之間的蜿蜒小巷，或者是從前的交通要道。而在眾多巷道之中，鹿港有著一條特殊的防火巷，吸引許多人前來一探究竟，成為著名的觀光景點，名為「摸乳巷」。
彩虹新組合　意義大不同
(2011-04-10)
陳蕾
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Department of Communication and Technology
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傳播與科技學系
彩虹一直以來象徵著同志的力量，但要不是吉爾伯特．貝克賦予彩虹生命意義，彩虹便不會如此盛行。不過最一開始的彩虹並非我們普遍認知的紅、橙、黃、綠、藍、靛、紫，是由亮粉紅、紅、橙、黃、綠、青綠、靛青、紫羅蘭組成，而後因為各顏色染料取之難易不同而有所改變。
「醫」與「病」不容忽視的平衡
(2011-04-10)
林映綺
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Department of Communication and Technology
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傳播與科技學系
現代醫學教育的起源，由奧斯勒醫師於十九世紀末創立，當時的醫學教育體系只指導醫學生在課堂上的知識，因此他首先提倡「臨床教育」。臨床教育不只提供了醫科學生在實務經驗上的累積，也幫助醫病關係的學習。
今非昔比社會成就戲劇
(2011-04-10)
王雅涵
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Department of Communication and Technology
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傳播與科技學系
傳統的布袋戲將人物大致分成生旦淨末丑五類，當然電視布袋戲在多年來與時俱進的發展下已不完全遵照原始的角色定位，人物的設計會逐漸超出預定，也不難由此看出時代正在改變的軌跡。
都市設計妙感
(2011-04-10)
蔡妙鈴
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Department of Communication and Technology
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傳播與科技學系
建築是有生命的，它雖然是凝固的，可在它上面蘊含著人文思想。—貝聿銘建築是體現在他自己的世界中的自我意識，有什麼樣的人，就有什麼樣的建築。—Frank Lloyd Wright 狗窩之所以為狗窩，就是因為住在裡面的是狗，而不是鳳凰還是恐龍。—Suzu
承載記憶與情感的汗水
(2011-04-10)
劉啟禾
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Department of Communication and Technology
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傳播與科技學系
火車串聯著城市和鄉村，火車承載著記憶與情感。人們搭火車駛向目的地，而這一趟趟的旅程，背後究竟隱藏了多少汗水？火車為什麼能這麼乾淨又穩定呢？
桃釋性感　舞出態度
(2011-04-10)
徐子晴
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Department of Communication and Technology
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傳播與科技學系
「可是性感哪裡有錯？我是一個女人，為什麼不能表現我自己的性感？」小桃說，「不脫衣服，純粹用身體語言去傳達性感的概念，才是真正的性感。」
不懈積累看文學開展眼前
(2011-04-10)
劉芮菁
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Department of Communication and Technology
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傳播與科技學系
從過去到現在，我一直和文學牽扯不清，這一切，都要話說從頭。
生產鏈角落的精神
(2011-04-10)
張芳瑜
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Department of Communication and Technology
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傳播與科技學系
職業有千千百百種，和工作環境較差的工廠相比，多數人嚮往的工作環境無非是舒適的辦公室。而需要付出勞動力且工作環境也比辦公室差的工廠，卻是在民國70至80年代代起台灣經濟的重要角色，但隨著台灣經濟發展，越來越多的小工廠遷出台灣，工人們在工廠裡揮汗的身影也越來越少見了。
旅行的意義
(2011-04-10)
陳俐吟
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Department of Communication and Technology
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傳播與科技學系
你一定以為，她是個不愛旅行的人吧？我也曾經這樣問過她。
望進純真靈魂深處：牠和我
(2011-04-10)
黃萱如
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Department of Communication and Technology
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傳播與科技學系
你知道，我很愛你，就像你很愛我一樣。
高天富用愛搭起族群橋梁
(2011-04-10)
陳文玲
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Department of Communication and Technology
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傳播與科技學系
當知道自己的祖先曾被原住民獵人頭—出草，而自己是被遺留下來的後代，面對過去泰雅族和客家人間的種族仇恨，錯綜複雜的族群糾葛，這其中微妙的情感牽絆著高天富，對於自我身分的認同又該如何建構？