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LEIOMYOMA AND VASCULAR ENDOTHELIAL GROWTH FACTOR GENE POLYMORPHISMS: A SYSTEMATIC REVIEW
(2010-09-01)
Chang, Chi Chen
;
Hsieh, Yao Yuan
;
Lin, Wen Hsin
;
Lin, Chih-Sheng
;
生物科技學系
Leiomyomas (myoma or fibroid) are the most common gynecologic tumors that occur in women of reproductive age, but their molecular pathogenesis is still unknown Since the growth of leiomyomas involve numerous vascular factors, an association between the leiomyoma and growth factors is suspected Vascular endothelial growth factor (VEGF) is one of the most important angiogenic growth factors VEGF regulates angiogenesis and mediates sex steroid induced cell growth and differentiation VEGF mediated activities seem to contribute to the pathogenesis of leiomyoma Genetic variations, including polymorphisms, in VEGF might also be associated with the complex pathogenesis of leiomyomas Here, we performed a systematic review of the roles of VEGF and its polymorphisms in the pathogenesis of leiomyoma [Taiwan J Obstet Gynecol 2010,49(3) 247-253]
Crystal polymorphism of poly(butylene-2,6-naphthalate) prepared by thermal treatments
(2002-03-01)
Ju, MY
;
Huang, JM
;
Chang, FC
;
應用化學系
;
Department of Applied Chemistry
The crystal polymorphisms of the poly(butylene-2,6-naphthalate) (PBN) prepared by various thermal treatments in bulk state were investigated. Crystalline forms obtained from different thermal treatments were characterized using wide-angle X-ray diffraction (WAXD) measurements. The a-form crystal is obtained by annealing the quenched PBN sample in the solid state or by crystallizing from the static melt at a relatively lower temperature (T-C < 205 degreesC). On the other hand, an exclusive beta-form crystal is generated through unique thermal treatment by non-isothermal crystallization at a cooling rate of 0.1 degreesC min(-1) from 280 to 25 degreesC. Additionally, both alpha- and beta-form crystals appear in the sample melt-crystallized at a relatively higher temperature, and the relative ratio of the a- and P-forms depends on the predetermined crystallization temperature. In this study, it was found that the crystalline form transition never occurs in the solid state. Optical microscopic observations indicate that the a-form crystal exhibits a typical spherulite morphology, while the P-form crystal displays a dendritic spherulite morphology due to the extremely slow spherulite growth rate. Fourier transform infrared spectroscopy was used to examine the crystal structures of both a- and P-forms of the PBN. It is proposed that the main difference between these two crystal structures of PBN prepared by different thermal treatments lies in the packing efficiency of the crystal chains, rather than in the conformation change of the glycol residue, with the beta-form possessing tighter packing efficiency than the alpha-form. (C) 2002 Elsevier Science Ltd. All rights reserved.
Polymorphisms of the beta(2)-Adrenergic Receptor Correlated to Nocturnal Asthma and the Response of Terbutaline Nebulizer
(2011-02-01)
Lee, Ming-Yung
;
Cheng, Shin-Nan
;
Chen, Shyi-Jou
;
Huang, Hui-Ling
;
Wang, Chih-Chien
Background: Inhaled beta(2)-adrenergic receptor (beta(2)-AR) agonists are the mainstay of treatment of acute asthma. Polymorphisms of the beta(2)-AR, especially codons 16, 27, and 164, may affect the functions of the receptor. This study was conducted to investigate whether different polymorphisms of the beta(2)-AR are related to the treatment responses of an inhaled beta(2)-AR agonist in children with nocturnal and nonnocturnal asthma in Taiwan. Methods: The nocturnal asthma group consisted of 27 children (mean age of 10.3 +/- 2.4 years), and the nonnocturnal asthma group consisted of 24 patients (mean age of 9.9 +/- 3.0 years). Allele-specific polymerase chain reaction was performed to determine 16, 27, and 164 loci alleles of beta(2)-AR genetic polymorphisms, and peak expiratory flow (PEF) was measured before and 1 hour after inhalation of 0.2 mg/kg/dose of terbutaline to determine the treatment response in these patients. Results: The polymorphisms of beta(2)-AR 27 but not 16 or 164 were significantly associated with the response to terbutaline nebulizer (p < 0.05). The polymorphism of beta(2)-AR 16 was associated with nocturnal asthma (p = 0.027). The Gly16 allele was more prevalent in the nocturnal asthma group (9/27; 33.3%) than in the nonnocturnal asthma group (3/24; 12.5%). Arg16 allele was less prevalent in the nocturnal asthma (3/27; 11.1%) than in the nonnocturnal asthma group (10/24; 41.7%). There was also a linkage disequilibrium found between beta(2)-AR 16 (Arg/Arg) and beta(2)-AR 27 (Gln/Gln). Conclusion: These findings suggest that polymorphisms of beta(2)-AR 16 are related to nocturnal asthma and polymorphisms of beta(2)-AR 27 are associated with the variable responses to the inhaled terbutaline in children with nocturnal and nonnocturnal asthma. Copyright (C) 2011, Taiwan Pediatric Association. Published by Elsevier Taiwan LLC. All rights reserved.
Angiotensin I-converting enzyme ACE 2350*G and ACE-240*T-related genotypes and alleles are associated with higher susceptibility to endometriosis
(2005-01-01)
Hsieh, YY
;
Chang, CC
;
Tsai, FJ
;
Hsu, CM
;
Lin, CC
Endometriosis displays features similar to malignancy, ranging from neovascularization to local invasion and aggressive spread to distant organs. The altered vascular-related genes might be related to the development of endometriosis. This study investigates whether angiotensin I-converting enzyme (ACE) *A2350G and A-240T gene polymorphisms could be used as markers of susceptibility in endometriosis. Women were divided into two groups: (1) endometriosis group (n=150) and (2) non-endometriosis group (n=159). Genomic DNA was obtained from peripheral leukocytes. ACE A2350G and A-240T gene polymorphisms were amplified by PCR and detected after restriction enzyme digestion with BstUI and XbaI. Genotypes and allelic frequencies in both groups were compared. We observed that genotype distribution and allele frequency of ACE 2350 and ACE-240 gene polymorphisms in both groups were significantly different. Proportions of ACE 2350*A homozygote/heterozygote/G homozygote in both groups were: (1) 66.7/29.3/4% and (2) 96.2/3.1/0.7%. Proportions of ACE-240*A homozygote/heterozygote/T homozygote in both groups were: (1) 43.3/46/10.7% and (2) 62.9/35.8/1.3%. We concluded that ACE 2350*G and ACE-240*T-related genotypes and alleles are associated with higher susceptibility to endometriosis. ACE A2350G and A-240T gene polymorphisms might be associated with endometriosis development.
Laser-Induced Crystallization and Crystal Growth
(2011-11-04)
Sugiyama, Teruki
;
Masuhara, Hiroshi
;
應用化學系
;
應用化學系分子科學碩博班
;
Department of Applied Chemistry
Recent streams of laser studies on crystallization and crystal growth are summarized and reviewed. Femtosecond multiphoton excitation of solutions leads to their ablation at the focal point, inducing local bubble formation, shockwave propagation, and convection flow. This phenomenon, called "laser micro tsunami" makes it possible to trigger crystallization of molecules and proteins from their supersaturated solutions. Femtosecond laser ablation of a urea crystal in solution triggers the additional growth of a single daughter crystal. Intense continuous wave (CW) near infrared laser irradiation at the air/solution interface of heavy-water amino acid solutions results in trapping of the clusters and evolves to crystallization. A single crystal is always prepared in a spatially and temporally controlled manner, and the crystal polymorph of glycine depends on laser power, polarization, and solution concentration. Upon irradiation at the glass/solution interface, a millimeter-sized droplet is formed, and a single crystal is formed by shifting the irradiation position to the surface. Directional and selective crystal growth is also possible with laser trapping. Finally, characteristics of laser-induced crystallization and crystal growth are summarized.
Polymorphism for transforming growth factor beta 1-509 (TGF-B1-509): Association with endometriosis
(2005-06-01)
Hsieh, YY
;
Chang, CC
;
Tsai, FJ
;
Peng, CT
;
Yeh, LS
Transforming growth factor beta (TGF-B) family members are multi-functional cytokines that play a key role in cellular growth, proliferation, and differentiation. The aim of the study was to investigate whether the TGF-B1-509 gene polymorphism could be used as a marker of susceptibility in endometriosis. Women were divided into two groups: endometriosis (n = 150) and non-endometriosis (n = 159). Polymorphisms for TGF-B1-509 genes were amplified by polymerase chain reaction and detected after restriction enzyme digestion. Genotypes and allelic frequencies in both groups were compared. Genotype proportions and allele frequencies of TGF-B1 gene polymorphisms differed significantly in both groups. Proportions of C homozygote, heterozygote, and T homozygote for TGF-B1 gene polymorphisms were 9.3/61.3/29.4% in the endometriosis group and 41.3/58.5/0% in the non-endometriosis group. Alleles C and T for TGF-B1 gene polymorphism were 40/60% (endometriosis) and 70.8/29.2% (non-endometriosis). Association of endometriosis with TGF-B1-509 gene polymorphism exists. T homozygote and T allele for TGF-B1 are associated with higher susceptibility to endometriosis.
Estrogen receptor alpha-351 XbaI*G and -397 PvuII*C-related genotypes and alleles are associated with higher susceptibilities of endometriosis and leiomyoma
(2007-02-01)
Hsieh, Y. -Y.
;
Wang, Y. -K.
;
Chang, C. -C.
;
Lin, C. -S.
;
生物科技學系
Endometriosis and leiomyoma are both common estrogen-related gynaecological diseases. We aimed to elucidate the association of estrogen receptor alpha (ER alpha)-351 A > G (XbaI) and -397 T > C (PvuII) gene polymorphisms with endometriosis and leiomyoma. Women were divided into three groups: (i) severe endometriosis (n = 112), (ii) leiomyoma (n = 106) and (iii) normal controls (n = 110). Genomic DNA was obtained from peripheral leukocytes. ER alpha-351 A/G XbaI and -397 T/C PvuII polymorphisms were assayed by the method of PCR and restriction fragment length polymorphism (RFLP). Genotypes and allelic frequencies in each group were compared. The genotype/allele frequencies of ER alpha-351 and -397 polymorphisms in endometriosis or leiomyoma groups were different from those of normal controls. ER alpha mutant-related genotypes/alleles (-351G and -397C) presented higher percentages in the endometriosis/leiomyoma population compared with normal controls. Proportions of ER alpha-351 AA/AG/GG genotypes and A/G alleles in each group were (i) 26.8/57.1/16.1 and 55.4/44.6%; (ii) 19.8/52.8/27.4 and 46.2/53.8% and (iii) 33.6/64.6/1.8 and 65.9/34.1%. Proportions of ER alpha-397 TT/TC/CC genotypes and T/C alleles in each group were (i) 24.1/60.7/15.2 and 54.5/45.5%; (ii) 23.6/70.8/5.6 and 59/41% and (iii) 54.5/40/5.5 and 74.5/25.5%. We concluded that ER alpha-351 XbaI*G- and -397 PvuII*C-related genotypes/alleles were correlated with higher susceptibilities of endometriosis or leiomyoma, which might be associated with related pathogeneses.
Angiotensin I-Converting enzyme insertion-related genotypes and allele are associated with higher susceptibility of endometriosis and leiomyoma
(2007-07-01)
Hsieh, Yao-Yuan
;
Lee, Cheng-Chun
;
Chang, Chi-Chen
;
Wang, Yu-Kuo
;
Yeh, Lian-Shun
Endometriosis and leiomyoma display features similar to malignancy, requiring neovascularization to proliferation and growth. Altered vascular-related genes might be related to the development of endometriosis and leiomyoma. Polymorphisms of the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) genes have been linked with some vascular diseases. This study investigates whether ACE I/D gene polymorphisms could be used as markers of susceptibility in endometriosis and leiomyoma. Women were divided into three groups: (4) endometriosis (n = 125); (2) leiomyoma (n = 120); () normal controls (n = 128). Genomic DNA was obtained from peripheral leukocyte. ACE I/D gene polymorphisms in intron 16 were amplified by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) Genotypes and allelic frequencies in both groups were compared. We observed the genotype distribution and allele frequency of ACE I/ D gene polymorphisms in both groups were significantly different. Proportions of ACE(star)I homozygote/ heterozygote/D homozygote in both groups were: (1) 50.4/24/25.6%; (2) 25/23.33/51.67%; (3) 10.2/ 29.7/60.1%. Proportions of I/D alleles in each group Were: (1) 62.4/37.6%; (2) 36.7/63.3%; (3) 25/75%. We concluded that ACE(star)I/D gene polymorphisms are associated with endometriosis and leiomyoma suspeptibilities. ACE(star)I-related genotypes and allele are strongly related to the occurrence of endometriosis and moderately related to the occurrence of leiomyoma.
Four novel single nucleotide Polymorphisms within the promoter region of p53 gene and their associations with uterine leiomyoma
(2007-07-01)
Hsieh, Yao-Yuan
;
Wang, Jui-Ping
;
Lin, Chich-Sheng
;
生物科技學系
;
Department of Biological Science and Technology
Dysregulated p53 expression has been implicated as a major contributor to numerous tumorigenesis. Single nucleotide polymorphisms (SNPs) within the functional consequence of the novel p53 promoter region remains obscure. Herein, we aimed to establish the extent of genetic variability within the promoter region of p53 gene as well as their association with leiomyoma susceptibility. Women were divided into two groups, leiomyoma (n = 160) and nonleiomyoma controls (n = 200). Total DNA was isolated from the peripheral blood of subjects. The DNA fragment containing p53 promoter regions (+64 similar to -404 bp) were obtained by amplification of polymerase chain reaction. The variations of DNA fragments were detected by DNA sequencing or restriction fragment length polymorphism (RFLP). Sequence alignment was used to identify sequence variations in p53 promoter regions. Genotypes were analyzed by method of RFLP. Genotypes/allelic frequencies in the leiomyoma and control groups were compared. A total of 15 sequence variations within p53 promoter region were identified, including -408 T/C, -382 A/G, -359 A/G, -325 T/C, -250 A/G, -216T/C, -205G/A, -198G/A, -177T/C, -103A/ G, -81 G/A, -71 G/A, -51 T/A, -33 A/G, and -17 T/ C. Among these variations, four SNPs (-250 A/G, -216 T/C, -103 A/G, and -33 A/G) were established. Allele frequencies of -250(star)G/-216(star)C/ -103(star)G/-33(star)G in the leiomyoma group and control group 6.9/5.0/5.9/3.8% and 3.8/1.8/2.3/4.0%, respectively. Two of them (-216(star)C and -103(star)G) are associated with higher leiomyoma susceptibility. We concluded that some sequence variations were observed within the promoter region of p53 gene. The SNPs of -216(star)C and -103(star)G among the identical sequence variations are associated with leiomyoma development.
XRCC4 codon 247*A and XRCC4 promoter-1394*T related genotypes but not XRCC4 intron 3 gene polymorphism are associated with higher susceptibility for endometriosis
(2008-05-01)
Hsieh, Yao-Yuan
;
Bau, Da-Tian
;
Chang, Chi-Chen
;
Tsai, Chang-Hai
;
Chen, Chih-Ping
DNA repair systems act to maintain genome integrity in the face of replication errors, environmental insults, and the cumulative effects of age. Genetic variants in DNA repair genes such as X-ray repair cross-complementing group 4 (XRCC4) might influence the ability to repair damaged DNA. Herein we aimed to investigate whether some XRCC4-related polymorphisms were associated with endometriosis susceptibility. Women were divided: (1) severe endometriosis (rAFS stage IV, n = 136) and (2) nonendometriosis groups (n = 112). The polymorphisms of XRCC4 codon 247, XRCC4 promoter - 1394, and XRCC4 intron 3 insertion/deletion (I/D) polymorphism were amplified by PCR and detected by electrophoresis after restriction enzyme (BBS I, Hinc II) digestions. Genotypes and allelic frequencies in both groups were compared. We observed that XRCC4 codon 247*A and XRCC4 promoter - 1394*T related genotypes, but not XRCC4 intron 3 I/D polymorphism, are associated with higher susceptibility for endometriosis. Distributions of XRCC4 codon 247*C homozygote/heterozygote/A homozygote, and C/A allele in both groups were: (1) 89/9.5/1.5% and 93.7/6.3%; (2) 97.3/2.7/0%, and 98.7/1.3% (P < 0.05). Proportions of XRCC4 promoter -1394*T homozygote/heterozygote/G homozygote and T/Gallelein both groups were: (1) 94.1/5.2/0.7% and 96.7/3.3%, and (2) 79.4/17.9/2.7% and 88.4/11.6% (P < 0.005). Proportions of XRCC4*1 homozygote/heterozygote/D homozygote and A/C allele in both groups were: (1) 67.6/30.9/1.5% and 83.2/16.8%, and (2) 70.5/24.1/5.4% and 82.6/17.4% (nondifference). We conclude that XRCC4 codon 247*A and XRCC4 promoter -1394*T related genotypes and alleles, but not XRCC4 intron 3 I/D polymorphism, might be associated with endometriosis susceptibilities and pathogenesis.

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