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Author: search.filters.author.Liu, Chih-TungSubject: search.filters.subject.PLA

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Now showing 1 - 3 of 3
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    Enhancement of cell growth on honeycomb-structured polylactide surface using atmospheric-pressure plasma jet modification
    (2017-02-01)
    Cheng, Kuang-Yao
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    Chang, Chia-Hsing
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    Yang, Yi-Wei
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    Liao, Guo-Chun
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    Liu, Chih-Tung
    In this paper, we compare the cell growth results of NIH-3T3 and Neuro-2A cells over 72 h on flat and honeycomb structured PLA films without and with a two-step atmospheric-pressure nitrogen-based plasma jet treatment. We developed a fabrication system used for forming of a uniform honeycomb structure on PLA surface, which can produce two different pore sizes, 3-4 mu m and 7-8 mu m, of honeycomb pattern. We applied a previously developed nitrogen-based atmospheric-pressure dielectric barrier discharge (DBD) jet system to treat the PLA film without and with honeycomb structure. NIH-3T3 and a much smaller Neuro-2A cells were cultivated on the films under various surface conditions. The results show that the two-step plasma treatment in combination with a honeycomb structure can enhance cell growth on PLA film, should the cell size be not too smaller than the pore size of honeycomb structure, e.g., NIH-3T3. Otherwise, cell growth would be better on flat PLA film, e.g., Neuro-2A. (C) 2016 Elsevier B.V. All rights reserved.
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    An Optimized Two-Step Nitrogen-Based Plasma Treatment Procedure under Atmospheric-Pressure Condition and its Surface Amination Mechanism
    (2015-07-01)
    Yang, Yi-Wei
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    Wu, Jane-Yii
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    Liu, Chih-Tung
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    Liao, Guo-Chun
    ;
    Cheng, Kuang-Yao
    Atmospheric pressure nitrogen-based dielectric barrier discharge (DBD) jet with two-step plasma treatment procedure has been verified to be very effective in facilitating nitrogen-containing group incorporation on Polylactide (PLA) surface. It includes: 1) pretreatment plasma treatment by a nitrogen discharge with a trace amount of oxygen, and 2) ammonia plasma treatment by a nitrogen discharge with a small amount of ammonia. In this study, the optimized operation condition of two-step plasma procedure is investigated under different concentrations of added gases (0-1% oxygen; 0-10% ammonia). XPS analysis shows that approximate to 0.19 of N/C ratio can be achieved by means of the optimized two-step plasma procedures. Furthermore, a surface amination mechanism is proposed based on the observations of Optical Emission Spectra (OES), Atomic Force Microscope (AFM), and X-ray Photoelectron Spectra (XPS) analyses.
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    Fast incorporation of primary amine group into polylactide surface for improving C2C12 cell proliferation using nitrogen-based atmospheric-pressure plasma jets
    (2014-01-01)
    Yang, Yi-Wei
    ;
    Wu, Jane-Yii
    ;
    Liu, Chih-Tung
    ;
    Liao, Guo-Chun
    ;
    Huang, Hsuan-Yu
    In this article, we report the development of the fast incorporation of primary amine functional groups into a polylactide (PLA) surface using the post-discharge jet region of an atmospheric-pressure nitrogen-based dielectric barrier discharge (DBD). Plasma treatments were carried out in two sequential steps: (1) nitrogen with 0.1% oxygen addition, and (2) nitrogen with 5% ammonia addition. The analyses show that the concentration of N/C ratio, surface energy, contact angle, and surface roughness of the treated PLA surface can reach 19.1%, 70.5 mJ/m(2), 38 degrees and 73.22 nm, respectively. In addition, the proposed two-step plasma treatment procedure can produce a PLA surface exhibiting almost the same C2C12 cell attachment and proliferation performance as that of the conventional gelatin coating method. Most importantly, the processing/preparation time is reduced from 13-15 h (gelatin coating method) to 5-15 min (two-step plasma treatment), which is very useful in practical applications. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 160-169, 2014.