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Hydrogen Bonding-Induced H-Aggregation for Fluorescence Turn-On of the GFP Chromophore: Supramolecular Structural Rigidity
(1970-01-01)
Tsai, Meng-Shiue
;
Tsai, Sung-Yu
;
Huang, Yi-Fan
;
Wang, Chien-Lung
;
Sun, Shih-Sheng
To turn on the fluorescence of the native green fluorescence protein (GFP) chromophore, 4-hydroxybenzylidene-dimethylimidazolinone (HBDI), in an artificial supramolecular system has been a challenging task, because it requires high local environmental rigidity. This work shows that the formation of H-aggregates of an HBDI-containing organogelator results in two orders of magnitude fluorescence enhancement (phi(f)=2.9 vs. 0.02 %), in which the inter-HBDI OH...OH H-bonds play a crucial role. The aggregation-induced fluorescence enhancement of HBDI has important implications on the origin of the high fluorescence quantum efficiency of HBDI in the GFP beta-barrel and on the supramolecular strategy for a full fluorescence recovery of HBDI. These results reveal a new approach to designing rigid chromophore aggregates for high-performance optoelectronic properties.
Cascade One-Pot Synthesis of Orange-Red-Fluorescent Polycyclic Cinnolino[2,3-f]phenanthridin-9-ium Salts by Palladium(II)-Catalyzed C-H Bond Activation of 2-Azobiaryl Compounds and Alkenes
(2020-01-07)
Jayakumar, Jayachandran
;
Vedarethinam, Guganchandar
;
Hsiao, Huan-Chang
;
Sun, Shang-You
;
Chuang, Shih-Ching
Quaternary ammonium salts were synthesized in moderate to good yields through double oxidative C-H bond activation on azobenzenes. The mechanism of the highly regioselective reaction of 2-azobiaryls with alkenes to give orange-red-fluorescent cinnolino[2,3-f]phenanthridin-9-ium salts and 15H-cinnolino[2,3-f]phenanthridin-9-ium-10-ide is proposed to involve ortho C-H olefination of the 2-azobiaryl compound with the alkene, intramolecular aza-Michael addition, concerted metalation-deprotonation (CMD), reductive elimination, and oxidation.
Cascade One-Pot Synthesis of Orange-Red-Fluorescent Polycyclic Cinnolino[2,3-f]phenanthridin-9-ium Salts by Palladium(II)-Catalyzed C-H Bond Activation of 2-Azobiaryl Compounds and Alkenes
(1970-01-01)
Jayakumar, Jayachandran
;
Vedarethinam, Guganchandar
;
Hsiao, Huan-Chang
;
Sun, Shang-You
;
Chuang, Shih-Ching
Quaternary ammonium salts were synthesized in moderate to good yields through double oxidative C-H bond activation on azobenzenes. The mechanism of the highly regioselective reaction of 2-azobiaryls with alkenes to give orange-red-fluorescent cinnolino[2,3-f]phenanthridin-9-ium salts and 15H-cinnolino[2,3-f]phenanthridin-9-ium-10-ide is proposed to involve ortho C-H olefination of the 2-azobiaryl compound with the alkene, intramolecular aza-Michael addition, concerted metalation-deprotonation (CMD), reductive elimination, and oxidation.
Fluorescent B-10 embedded nanodiamonds as B-10 delivery agent for boron neutron capture therapy
(2019-06-26)
Lin, Bo-Rong
;
Kunuku, Srinivasu
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Chen, Tzung-Yuang
;
Chang, Yu-Jen
;
Liao, Li-Chuan
Boron neutron capture therapy is a potentially powerful anti-cancer treatment, the success of which depends heavily on the boron delivery agent. Enabling the real-time tracing of delivery agents as they move through the body is crucial to the further development of boron neutron capture therapy. In this study, we fabricate fluorescent B-10 embedded nanodiamonds using physical ion implantation in conjunction with a two-step annealing process. Fluorescent B-10 embedded nanodiamonds perform well in cell viability test. The red fluorescence of the B-10 embedded nanodiamonds allows their use in fluorescence microscopy and in vivo imaging systems, thereby making it possible to conduct tracking in real time. The proposed fluorescent B-10 embedded nanodiamonds, combining optical visibility and B-10 transport capability, might be a promising boron delivery agent suitable for a wide range of biomedical applications.
Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS)
(2012-01-01)
Hsieh, Yao-Fang
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Chen, Chih-Hsien
;
Mang, Ou-Yang
;
Duann, Jeng-Ren
;
Chiou, Jin-Chern
Currently, the cancer was examined by diagnosing the pathological changes of tumor. If the examination of cancer can diagnose the tumor before the cell occur the pathological changes, the cure rate of cancer will increase. This research develops a human-machine interface for hyper-spectral microscope. The hyper-spectral microscope can scan the specific area of cell and records the data of spectrum and intensity. These data is helpful to diagnose tumor. This study finds the hyper-spectral imaging have two higher intensity points at 550nm and 700nm, and one lower point at 640nm between the two higher points. For analyzing the hyper-spectral imaging, the intensity at the 550nm peak divided by the intensity at 700nm peak. Finally, we determine the accuracy of detection by Gaussian distribution. The accuracy of detecting normal cells achieves 89%, and the accuracy of cancer cells achieves 81%.
Effect of atomic position on the spontaneous emission of a three-level atom in a coherent photonic-band-gap reservoir
(2009-01-01)
Cheng, Szu-
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Wu, Jing-Nuo
;
Yang, Tzong-Jer
;
Hsieh, Wen-Feng
;
光電工程學系
The effect of atomic position on the spontaneous emission and optical spectra of a three-level atom embedded in a photonic crystal was studied by considering the coherent two-band photonic-band-gap (PBG) reservoir. With the definite phase difference between the fields of the air band and dielectric band in this coherent PBG reservoir, we found that this coherent property grows stronger and results in the spectrum shift and the appearance of dark lines and kinks as the width of band gap gets smaller; and the variation of the atomic position leads to large shift of spectral peak. The averaged spectra of the system with an ensemble of atoms embedded in the dielectric region are blueshifted while those of atoms in the air region are redshifted. The spectra peaks can even shift more than 75% of the half width at half maximum. The feasible experimental systems composed of an ensemble of molecules (quantum dots or fluorescence dye) in photonic crystals are discussed.
Targeted RGD nanoparticles for highly sensitive in vivo integrin receptor imaging
(2012-01-01)
Lin, Ren-Yen
;
Dayananda, Kasala
;
Chen, Ting-Jung
;
Chen, Chiao-Yun
;
Liu, Gin-Chung
A new magnetic resonance imaging (MRI) contrast bearing RGD peptide is reported. In this study, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles with various sizes were prepared. Particles sizes between 6 and 13?nm were tuned by varying the stirring rate. Remarkable negative contrast was observed because USPIO nanoparticles (13.1?+/-?2.1?nm) generated high transversal relaxivity r2 (188?+/-?3?m m-1?s-1) and saturation magnetization (94?emu?g-1 Fe). The USPIO nanoparticles were coated with PDA [2-(pyridyldithio)-ethylamine; PDA nanoparticles] containing functional polymer, which can be readily synthesized by Michael addition. The PDA nanoparticles were conjugated with RGD peptide (RGD nanoparticles) for targeting the specific site. The target specificity and high relaxivity allowed RGD nanoparticles to differentiate the expression level of integrin receptor on several cell lines and tumors (MCF-7, A-549, HT-29 and HT-1080) by in vitro and in vivo MR imaging. Importantly, a remarkable negative contrast (-51.3?+/-?6.7%) was observed for in vivo MR imaging of MCF-7 tumor. This result implies that the RGD nanoparticles that greatly enhance the MR imaging are highly sensitive for early stage tumor detection. Copyright (c) 2012 John Wiley & Sons, Ltd.
Plate-based biochemical assay using quantum dots as a fluorescent labeling agent
(2005-07-22)
Huang, CP
;
Liu, HW
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Tsao, CY
;
Yin, LT
;
Chiu, SF
A biochemical assay based on fluorescence imaging analysis by confocal laser scanning as a detection technique is specified. The method involved a glass plate coated with nitrocellulose (NC), where alpha-human-IgE-biotin at various concentrations was immobilized on an NC plate. Luminescent core-shell CdSe/ZnS QD-SA conjugates were then used as a fluorescent labeling agent to be captured specifically by biotinated alpha-human-IgE immobilized in a micro-array by affinity binding between SA and biotin in a plate-based biochemical assay. A confocal laser scanner was used to detect the fluorescence signals from the alpha-human-IgE-biotin-SA-QD complex. Experimental findings reveal that fluorescence intensity of QD-SA saturated at QD concentrations above 0.4 mg/ml. Moreover, a calibration curve between the fluorescence intensity and the concentration of alpha-human-IgE-biotin is plotted. The range of detectable concentrations of biotinlated alpha-human IgE was found to be between 3.3 and 100 mu g/ml in the a-human-IgE-biotin-SA-QD complex. Therefore, the results were consistent with the binding specificity in a plate-based biochemical assay, as determined using luminescent QDs as a labeling agent. (c) 2004 Elsevier B.V. All rights reserved.
Blue Fluorescence and Bipolar Transport Materials Based on Anthracene and Their Application in OLEDs
(2012-06-01)
Ho, Meng-Huan
;
Balaganesan, Banumathy
;
Chen, Chin H. (Fred)
;
交大名義發表
;
National Chiao Tung University
The phenomenon of organic electroluminescence (EL) was first discovered from studies on anthracene crystals in the 1960s. Since then, its derivatives have been studied extensively in applications such as organic light-emitting diodes (OLEDs) and organic thin-film transistors because of their excellent EL, transport, and good electrochemical properties. A blue fluorescence emitter based on anthracene is also important in constructing hybrid tandem white OLEDs for lighting applications. This article provides a review about the development of blue host OLED materials based on molecules designed and derived from the core structure of anthracene and their application as bipolar charge-carrier transport materials in OLEDs. The recent development of efficient, stable, blue-doped p-i-n OLEDs with simplified device architecture based on the single common host 2-methyl-9,10-di(2-napthyl)anthracene, with its stable thin-film morphology, large band gap energy, high fluorescence quantum yield, and ambipolar charge-carrier transport properties, is also highlighted.
N-Doped Graphene Quantum Dots-Decorated V2O5 Nanosheet for Fluorescence Turn Off-On Detection of Cysteine
(2018-01-10)
Ganganboina, Akhilesh Babu
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Chowdhury, Ankan Dutta
;
Doong, Ruey-An
;
環境工程研究所
;
Institute of Environmental Engineering
The development of a fast-response sensing technique for detection of cysteine can provide an analytical platform for prescreening of disease. Herein, we have developed a fluorescence turn off-on fluorescence sensing platform by combining nitrogen-doped graphene quantum dots (N-GQDs) with V2O5 nanosheets for the sensitive and selective detection of cysteine in human serum samples. V2O5 nanosheets with 2-4 layers are successfully synthesized via a simple and scalable liquid exfoliation method and then deposited with 2-8 nm of N-GQDs as the fluorescence turn off-on nanoprobe for effective detection of cysteine in human serum samples. The V2O5 nanosheets serve as both fluorescence quencher and cysteine recognizer in the sensing platform. The fluorescence intensity of N-GQDs with quantum yield of 0.34 can be quenched after attachment onto V2O5 nanosheets. The addition of cysteine triggers the reduction of V2O5 to V4+ as well as the release of N-GQDs within 4 min, resulting in the recovery of fluorescence intensity for the turn off-on detection of cysteine. The sensing platform exhibits a two-stage linear response to cysteine in the concentration range of 0.1-15 and 15-125 mu M at pH 6.5, and the limit of detection is 50 nM. The fluorescence response of N-GQD@V2O5 exhibits high selectivity toward cysteine over other 22 electrolytes and biomolecules. Moreover, this promising platform is successfully applied in detection of cysteine in human serum samples with excellent recovery of (95 +/- 3.8) - (108 +/- 2.4)%. These results clearly demonstrate a newly developed redox reaction-based nanosensing platform using N-GQD@V2O5 nanocomposites as the sensing probe for cysteine-associated disease monitoring and diagnosis in biomedical applications, which can open an avenue for the development of high performance and robust sensing probes to detect organic metabolites.

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