标题: 探讨在双轴的力学环境下人造组织里产生组织结构的作用机制并建立组织结构与相对应的机械性质的关系
Exploring Mechanisms of Structural Organization Development in Engineered Tissues Subjected Biaxial Loading and Establishing the Relationship between the Structural Organization and the Corresponding Mechanical Properties
作者: 胡晋嘉
Hu Jin-Jia
国立交通大学机械工程学系(所)
公开日期: 2009
摘要: 组织工程有极大的潜力可提供具正常功能的组织或器官以供移植. 当所欲取代的
组织在体内具备机械方面的功能, 这些植入物的机械性质就非常重要; 他们的机械性
质须能与原來体内的组织相似. 我们提议建造能与非线性光学系统结合的双轴培养装
置, 并双轴往復致动器以供动态拉放凝胶, 及双轴机械性质测试机以取得凝胶的应力
与应变关系. 我们将使用不含支架之含纤维母细胞之胶原蛋白凝胶或含细胞之纤维蛋
白凝胶作为我们的组织模型. 我们将对凝胶在双轴力学环境下的组织结构的发展作了
解, 而因组织结构与其机械强度息息相关, 我们也会尝试建立兩者间之关聯. 因在此二
组织模型下, 胞外间质是由纤维母细胞所调控, 我们相信细胞与胞外间质间的互动关
系对于组织结构的发展有重要的影响. 所以我们会探讨造成细胞或纤维方向改变的机
制并检视在双轴力学环境下, 凝胶内细胞的移动行为. 这个计画若能执行成功, 将让我
们了解一个对力学的负载有反应的人造组织, 其发展中的组织结构与其机械性质的关
系. 更重要的, 我们会对如何操控双轴的力学环境而使我们的人造组织达到所求之机
械性质有更多的认識.
Tissue engineering has great potential to provide viable, functional tissue/organ for
transplantation. If tissues to be replaced have their important mechanical functions in the
body, the mechanical properties of the implants are often critical; the mechanical properties
of them should match that of the host tissue. We propose to build biaxial culturing chambers,
which can be incorporated into nonlinear optical microscopy system for imaging gels,
biaxial cyclic actuators, which allow gels to be cyclically stretched, and a biaxial mechanical
tester, which measures biaxial stress-strain behaviors of gels. Scaffold-free
fibroblasts-seeded collagen or fibrin gels will be served as the model tissues. We propose to
study the development of structural organization, which has an important role in mechanical
strength of a material, of model tissues under biaxial loading conditions and establish
correlation between the mechanical properties and the structural organization. As
extracellular matrix is regulated by embedded cells, we propose that the cell-ECM
interactions must play a significant role in determination of the structural organization. In
particular, we will explore mechanism behind cell/fiber orientations and examine cell
migration in gels subjected to biaxial loading. Successful completion of this project will
improve our understanding of the relationship between the evolving structural organization
and the bulk mechanical properties in load-responsive engineered tissues. More importantly,
we will know better how to manipulate biaxial loading conditions to achieve desired
mechanical behavior of engineered tissues.
官方说明文件#: NSC97-2218-E006-289-MY2
URI: http://hdl.handle.net/11536/101104
https://www.grb.gov.tw/search/planDetail?id=1763373&docId=301199
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