發展一套超高靈敏度且即時檢測流感病毒的奈米線場效電晶體生物感測器(I)

Abstract

本計畫之研究重點為結合分子檢測技術及奈米半導體元件，以開發一套具有超高靈敏度、無須標 的、可即時偵測、操作簡便的奈米線場效電晶體生物感測器。使用多晶矽奈米線場效電晶體為本計畫 之特色，由於其製備過程相容於現今商業化之半導體製程，研發完成之產品，將可結合台灣現有的半 導體技術來大量生產。我們將以禽流感病毒的RNA 序列及表面蛋白為偵測標的，將相對應之DNA 探針或單株抗體分別固定化於矽奈米線表面，在偵測標的物附著於奈米線表面時，奈米線場效電晶體 之電性將產生極為靈敏的變化，藉此得到高靈敏度與專一性之檢測。我們將先選擇幾種不同亞型(將 先以H5 與H7 為主)的禽流感病毒為測試目標，分別完成單獨的可行性測試，並進行臨床測試。同時， 將藉由改善元件特性及奈米線表面生物分子修飾效率，提高感測靈敏度與專一性。未來將開發成微陣 列及可攜式奈米線場效電晶體生物感測器，作為未來商業產品開發之基礎。

We propose the use of semiconductor device as transducer for molecular diagnosis to develop an ultrahigh sensitivity, label-free, real time detection and easy to use biosensing system using silicon nanowire field effect transistor (SNW FET). One of the unique features of this project is to use poly silicon nanowire field transistor (poly-SNW FET) for its fabrication is compatible with current commercial semiconductor process. It is important for the future products will rely on the availability of the SNW FET. We will immobilize DNA or antibody, which is complementary to influenza virus RNA or against influenza virus’ surface protein, respectively, on the nanowire surface of SNW FET. The high sensitive and high specificity diagnosis will be achieved when the complementary RNA or antigen protein interacts on the nanowire surface of SNW FET. The electronic response of SNW FET is very sensitive to the variation of charges on its surface. Avian influenza viruses will be our primary targets especially for H5 and H7 subtypes. In vitro test in the laboratory will be established by following the clinical test in collaboration with medical center. We will concurrently improve the sensing sensitivity through the fabrication of semiconductor chip and through the biomolecular modification of the nanowire surface. In the future, a portable diagnostic instrument with micro array SNW FET chip will be explored as the basis for commercial product development.