標題: Characterization of MRT, a new non-LTR retrotransposon in Monascus spp
作者: Chen, Yi-Pei
Tseng, Ching-Ping
Liaw, Li-Ling
Wang, Chun-Lin
Yuan, Gwo-Fang
生物科技學系
Department of Biological Science and Technology
關鍵字: bacterial artificial chromosome;Monascus pilosus;non-LTR retrotransposon;phylogenetic analysis
公開日期: 1-Oct-2007
摘要: A new non-LTR retrotransposon, named MRT, was discovered in the filamentous fungus Monascus pilosus BCRC38072. The entire nucleotide sequence of the MRT element was 5.5-kb long, including two open reading frames. These two ORFs showed homologies to gag-like and pol-like gene products, and an A-rich sequence at the 3' end of pol-like gene. ORF1 encoded a protein of 5 17 amino acids and contained a cysteine-rich zinc finger motif ORF2 encoded a protein of 1181 amino acids and contained apurinic/apyrimidinic endonuclease (APE), reverse transcriptase (RT), RNaseH domains, and a CCHC motif. The phylogenetic analyses demonstrated that the MRT element should be classified into the Tad1 clade. The results of Southern hybridizations showed that MRT elements were distributed within M pilosus, M ruber, M sanguineus, and M barkeri. In addition, the species of Monascus can be grouped by the presence or absence of MRT elements in the hybridization pattern according to phylogenctic subgroups established with the partial beta-tubulin gene.
URI: http://hdl.handle.net/11536/10258
ISSN: 1817-406X
期刊: BOTANICAL STUDIES
Volume: 48
Issue: 4
起始頁: 377
結束頁: 385
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