標題: Nanometer-sized diamond particle as a probe for biolabeling
作者: Chao, Jui-I.
Perevedentseva, Elena
Chung, Pei-Hua
Liu, Kuang-Kai
Cheng, Chih-Yuan
Chang, Chia-Ching
Cheng, Chia-Liang
生物科技學系
Department of Biological Science and Technology
公開日期: 1-九月-2007
摘要: A novel method is proposed using nanometer- sized diamond particles as detection probes for biolabeling. The advantages of nanodiamond's unique properties were demonstrated in its biocompatibility, nontoxicity, easily detected Raman signal, and intrinsic fluorescence from its natural defects without complicated pretreatments. Carboxylated nanodiamond's ( cND's) penetration ability, noncytotoxicity, and visualization of cND- cell interactions are demonstrated on A549 human lung epithelial cells. Protein- targeted cell interaction visualization was demonstrated with cND- lysozyme complex interaction with bacteria Escherichia coli. It is shown that the developed biomolecule- cND complex preserves the original functions of the test protein. The easily detected natural fluorescent and Raman intrinsic signals, penetration ability, and low cytotoxicity of cNDs render them promising agents in multiple medical applications.
URI: http://dx.doi.org/10.1529/biophysj.107.108134
http://hdl.handle.net/11536/10346
ISSN: 0006-3495
DOI: 10.1529/biophysj.107.108134
期刊: BIOPHYSICAL JOURNAL
Volume: 93
Issue: 6
起始頁: 2199
結束頁: 2208
顯示於類別:期刊論文


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