標題: | Nanometer-sized diamond particle as a probe for biolabeling |
作者: | Chao, Jui-I. Perevedentseva, Elena Chung, Pei-Hua Liu, Kuang-Kai Cheng, Chih-Yuan Chang, Chia-Ching Cheng, Chia-Liang 生物科技學系 Department of Biological Science and Technology |
公開日期: | 1-九月-2007 |
摘要: | A novel method is proposed using nanometer- sized diamond particles as detection probes for biolabeling. The advantages of nanodiamond's unique properties were demonstrated in its biocompatibility, nontoxicity, easily detected Raman signal, and intrinsic fluorescence from its natural defects without complicated pretreatments. Carboxylated nanodiamond's ( cND's) penetration ability, noncytotoxicity, and visualization of cND- cell interactions are demonstrated on A549 human lung epithelial cells. Protein- targeted cell interaction visualization was demonstrated with cND- lysozyme complex interaction with bacteria Escherichia coli. It is shown that the developed biomolecule- cND complex preserves the original functions of the test protein. The easily detected natural fluorescent and Raman intrinsic signals, penetration ability, and low cytotoxicity of cNDs render them promising agents in multiple medical applications. |
URI: | http://dx.doi.org/10.1529/biophysj.107.108134 http://hdl.handle.net/11536/10346 |
ISSN: | 0006-3495 |
DOI: | 10.1529/biophysj.107.108134 |
期刊: | BIOPHYSICAL JOURNAL |
Volume: | 93 |
Issue: | 6 |
起始頁: | 2199 |
結束頁: | 2208 |
顯示於類別: | 期刊論文 |