Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, Chen-Yu | en_US |
dc.contributor.author | Chen, Yu-Chie | en_US |
dc.date.accessioned | 2014-12-08T15:13:32Z | - |
dc.date.available | 2014-12-08T15:13:32Z | - |
dc.date.issued | 2007-08-03 | en_US |
dc.identifier.issn | 0021-9673 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1016/j.chroma.2007.05.026 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/10452 | - |
dc.description.abstract | Ochratoxin A (OTA) is a toxicant commonly present in many food products. Conventionally, immuno-affinity analysis is applied to rapidly screen the presence of OTA in food. However, antibodies are expensive. In this study, we present a new approach for selectively enriching OTA from aqueous samples using human serum albumin (HSA) bound magnetic beads as the affinity probes, followed by the analysis of CE/ESI-MS. In addition to demonstrating the feasibility of using the affinity probes to concentrate OTA, we also propose a rapid concentration and elution method for extraction, that is, OTA are extracted from aqueous samples by pipetting the samples in and out of a sample vial for 1 min followed by elution with pipetting for another minute. On the basis of the magnetic property, the affinity magnetic probe-target species could be rapidly isolated from the solution during the process of extraction and elution by magnetic separation. CE/ESI-MS, coupled by the electrodeless/sheathless interface, is used for the analysis of the samples. As this method features speed and cost-effectiveness, it is suitable for the purpose of rapid screening. In fact, the lowest detection limit for OTA is similar to 4 x 10(-3) mg/L. (c) 2007 Elsevier B.V. All rights reserved. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | CE-ESI-MS | en_US |
dc.subject | albumin | en_US |
dc.subject | magnetic beads | en_US |
dc.subject | ochratoxin A | en_US |
dc.title | Selective enrichment of ochratoxin A using human serum albumin bound magnetic beads as the concentrating probes for capillary electrophoresis/electrospray ionization-mass spectrometric analysis | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.chroma.2007.05.026 | en_US |
dc.identifier.journal | JOURNAL OF CHROMATOGRAPHY A | en_US |
dc.citation.volume | 1159 | en_US |
dc.citation.issue | 1-2 | en_US |
dc.citation.spage | 250 | en_US |
dc.citation.epage | 255 | en_US |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | 應用化學系分子科學碩博班 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.contributor.department | Institute of Molecular science | en_US |
dc.identifier.wosnumber | WOS:000248466800030 | - |
dc.citation.woscount | 16 | - |
Appears in Collections: | Articles |
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