標題: Selective enrichment of ochratoxin A using human serum albumin bound magnetic beads as the concentrating probes for capillary electrophoresis/electrospray ionization-mass spectrometric analysis
作者: Hong, Chen-Yu
Chen, Yu-Chie
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: CE-ESI-MS;albumin;magnetic beads;ochratoxin A
公開日期: 3-Aug-2007
摘要: Ochratoxin A (OTA) is a toxicant commonly present in many food products. Conventionally, immuno-affinity analysis is applied to rapidly screen the presence of OTA in food. However, antibodies are expensive. In this study, we present a new approach for selectively enriching OTA from aqueous samples using human serum albumin (HSA) bound magnetic beads as the affinity probes, followed by the analysis of CE/ESI-MS. In addition to demonstrating the feasibility of using the affinity probes to concentrate OTA, we also propose a rapid concentration and elution method for extraction, that is, OTA are extracted from aqueous samples by pipetting the samples in and out of a sample vial for 1 min followed by elution with pipetting for another minute. On the basis of the magnetic property, the affinity magnetic probe-target species could be rapidly isolated from the solution during the process of extraction and elution by magnetic separation. CE/ESI-MS, coupled by the electrodeless/sheathless interface, is used for the analysis of the samples. As this method features speed and cost-effectiveness, it is suitable for the purpose of rapid screening. In fact, the lowest detection limit for OTA is similar to 4 x 10(-3) mg/L. (c) 2007 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.chroma.2007.05.026
http://hdl.handle.net/11536/10452
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2007.05.026
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1159
Issue: 1-2
起始頁: 250
結束頁: 255
Appears in Collections:Articles


Files in This Item:

  1. 000248466800030.pdf

If it is a zip file, please download the file and unzip it, then open index.html in a browser to view the full text content.