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dc.contributor.authorHong, Chen-Yuen_US
dc.contributor.authorChen, Yu-Chieen_US
dc.date.accessioned2014-12-08T15:13:32Z-
dc.date.available2014-12-08T15:13:32Z-
dc.date.issued2007-08-03en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.chroma.2007.05.026en_US
dc.identifier.urihttp://hdl.handle.net/11536/10452-
dc.description.abstractOchratoxin A (OTA) is a toxicant commonly present in many food products. Conventionally, immuno-affinity analysis is applied to rapidly screen the presence of OTA in food. However, antibodies are expensive. In this study, we present a new approach for selectively enriching OTA from aqueous samples using human serum albumin (HSA) bound magnetic beads as the affinity probes, followed by the analysis of CE/ESI-MS. In addition to demonstrating the feasibility of using the affinity probes to concentrate OTA, we also propose a rapid concentration and elution method for extraction, that is, OTA are extracted from aqueous samples by pipetting the samples in and out of a sample vial for 1 min followed by elution with pipetting for another minute. On the basis of the magnetic property, the affinity magnetic probe-target species could be rapidly isolated from the solution during the process of extraction and elution by magnetic separation. CE/ESI-MS, coupled by the electrodeless/sheathless interface, is used for the analysis of the samples. As this method features speed and cost-effectiveness, it is suitable for the purpose of rapid screening. In fact, the lowest detection limit for OTA is similar to 4 x 10(-3) mg/L. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectCE-ESI-MSen_US
dc.subjectalbuminen_US
dc.subjectmagnetic beadsen_US
dc.subjectochratoxin Aen_US
dc.titleSelective enrichment of ochratoxin A using human serum albumin bound magnetic beads as the concentrating probes for capillary electrophoresis/electrospray ionization-mass spectrometric analysisen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.chroma.2007.05.026en_US
dc.identifier.journalJOURNAL OF CHROMATOGRAPHY Aen_US
dc.citation.volume1159en_US
dc.citation.issue1-2en_US
dc.citation.spage250en_US
dc.citation.epage255en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000248466800030-
dc.citation.woscount16-
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