顯微鏡在細胞學之應用

Abstract

課程首頁

本課程是由交通大學生物科技學系暨研究所提供。

Microscopy has become one of the most utilized tool in the biology field. It is essential for modern day biologists to learn the basics of microscopy and how it should be properly used in studying biological events. This course will cover most techniques used in a cell biology lab ranging from contrast-based illumination to specialized fluorescence microscopy. This class will also discuss the potential problem of scientific misconducts related to image manipulation.

顯微技術已成為研究生物學最重要的工具之一。擁有基礎顯微常識及了解如何正確的使用顯微技術是現今從事生物學研究人員所不可或缺的知識。本課程將教授現今細胞生物實驗室所使用之顯微技術，涵蓋範圍從基本對比顯影技術至特殊螢光顯微技術。本課程並會討論與影像處理有關的科學倫理問題。

課程目標/概述

Microscopy has become one of the most utilized tool in the biology field. It is essential for modern day biologists to learn the basics of microscopy and how it should be properly used in studying biological events. This course will cover most techniques used in a cell biology lab ranging from contrast-based illumination to specialized fluorescence microscopy. This class will also discuss the potential problem of scientific misconducts related to image manipulation.

顯微技術已成為研究生物學最重要的工具之一。擁有基礎顯微常識及了解如何正確的使用顯微技術是現今從事生物學研究人員所不可或缺的知識。本課程將教授現今細胞生物實驗室所使用之顯微技術，涵蓋範圍從基本對比顯影技術至特殊螢光顯微技術。本課程並會討論與影像處理有關的科學倫理問題。

課程章節

單元主題

Class 1：Introduction

Class 2：Light, color, and resolution

Class 3：Fundamental of Light Microscopy

Class 4：Optical Aberrations and Objective Lens

Class 5：Conjugate planes and Köhler Illumination

Class 6：Cell Culture and Live Cell Imaging

Class 7 and 8：Darkfield, Phase Contrast, Hoffman Modulation, and DIC

Class 9：Fluorescence Microscopy

Class 10：Confocal Fluorescence Microscopy

Class 11：Fluorescence Microscopy Techniques

Class 12：Digital CCD imaging

Class 13：Image Processing and Manipulation

課程書目

上課講義。

參考書目與網站

Optical Microscopy Primer (http://micro.magnet.fsu.edu/primer/)

Zeiss Microscopy Campus (http://zeiss-campus.magnet.fsu.edu/index.html)

iBiology Microscopy Course (http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course.html)

評分標準

項目

課堂回答

期中考

期末考

授課對象：碩士生

預備知識：普通生物學、物理學以及一學期的細胞生物學 (general biology, physics, and one semester of cell biology)