標題: The DNA-binding domain of CaNdt80p is required to activate CDR1 involved in drug resistance in Candida albicans
作者: Wang, Jang-Shiun
Yang, Yun-Liang
Wu, Chin-Jung
Ouyang, Karen J.
Tseng, Kuo-Yun
Chen, Chia-Geun
Wang, Hsin
Lo, Hsiu-Jung
生物科技學系
Department of Biological Science and Technology
公開日期: 1-Oct-2006
摘要: CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an eff lux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug resistance, chimeras of CaNdt80p and ScNdt80p were constructed. Interestingly, the DNA-binding domain of ScNdt80p could functionally complement that of CaNdt80p to activate CDR1p-lacZ in S. cerevisiae. Consistently, CaNdt80p containing a mutation in the DNA-binding domain failed to activate CDR1p-lacZ in S. cerevisiae. Furthermore, a copy of CaNDT80 with the same mutation also failed to complement the drug-sensitive phenotype caused by a null mutation in C. albicans. Thus, the DNA-binding domain of CaNdt80p is critical for its function in drug resistance in C. albicans.
URI: http://dx.doi.org/10.1099/jmm.0.46650-0
http://hdl.handle.net/11536/11698
ISSN: 0022-2615
DOI: 10.1099/jmm.0.46650-0
期刊: JOURNAL OF MEDICAL MICROBIOLOGY
Volume: 55
Issue: 10
起始頁: 1403
結束頁: 1411
Appears in Collections:Articles